Combination effect of cytochrome P450 1A1 gene polymorphisms on uterine leiomyoma: A case-control study

Saeedeh Salimi; Mojtaba Sajadian; Maryam Khodamian; Atefeh Yazdi; Soodabeh Rezaee; Abbas Mohammadpour-Gharehbagh; Mojgan Mokhtari; Minoo Yaghmaie

doi:10.17305/bjbms.2016.1245

Authors

Saeedeh Salimi Cellular and Molecular Research Center and Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, Zahedan, Iran
Mojtaba Sajadian Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
Maryam Khodamian Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan
Atefeh Yazdi School of Medicine, Zahedan University of Medical Sciences, Zahedan
Soodabeh Rezaee School of Medicine, Zahedan University of Medical Sciences, Zahedan
Abbas Mohammadpour-Gharehbagh Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan
Mojgan Mokhtari School of Medicine, Zahedan University of Medical Sciences, Zahedan; Zahedan University of Medical Sciences, Zahedan
Minoo Yaghmaie School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran

DOI:

https://doi.org/10.17305/bjbms.2016.1245

Keywords:

Cytochrome P450 1A1, haplotype, gene, polymorphism, uterine leiomyoma

Abstract

Uterine leiomyoma (UL) is an estrogen-dependent neoplasm of the uterus, and estrogen metabolizing enzymes affect its progression. This study aimed to evaluate the association between two single-nucleotide polymorphisms of cytochrome P450 1A1 (CYP1A1) gene and UL risk. The study consisted of 105 patients with UL and 112 healthy women as controls. Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene were analyzed by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods, respectively. The findings indicated no association between Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene and UL (p < 0.05). However, the combination effect of TT/AG genotypes of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms was associated with 4.3-fold higher risk of UL. In addition, haplotype analysis revealed that TG haplotype of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms could increase the UL risk nearly 4.9-fold. Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms of CYP1A1 gene were not associated with UL susceptibility; however, the combination of the TT/AG genotypes and TG haplotype could increase the UL risk.

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Author Biographies

Saeedeh Salimi, Cellular and Molecular Research Center and Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, Zahedan, Iran

Cellular and Molecular Research Center; Department of Clinical Biochemistry
Mojtaba Sajadian, Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Cellular and Molecular Research Center; Department of Clinical Biochemistry
Maryam Khodamian, Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan

Cellular and Molecular Research Center; Department of Clinical Biochemistry
Atefeh Yazdi, School of Medicine, Zahedan University of Medical Sciences, Zahedan

Department of Obstetrics and Gynecology
Soodabeh Rezaee, School of Medicine, Zahedan University of Medical Sciences, Zahedan

Department of Obstetrics and Gynecology
Abbas Mohammadpour-Gharehbagh, Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan

Cellular and Molecular Research Center; Department of Clinical Biochemistry
Mojgan Mokhtari, School of Medicine, Zahedan University of Medical Sciences, Zahedan; Zahedan University of Medical Sciences, Zahedan

Department of Obstetrics and Gynecology; Pregnancy Health Research Center
Minoo Yaghmaie, School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran

Department of Obstetrics and Gynecology

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