mRNA expression of nuclear factor of activated T-cells, cytoplasmic 2 (NFATc2) and peroxisome proliferator-activated receptor gamma (PPARG) transcription factors in colorectal carcinoma

Authors

  • Venus Zafari Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran http://orcid.org/0000-0003-0027-7029
  • Shahryar Hashemzadeh Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran; General and Vascular Surgery Department of Tabriz University of Medical Sciences, Tabriz, Iran
  • Mohammadali Hosseinpour Feizi Department of Animal Biology, University of Tabriz, Tabriz, Iran
  • Nasser Pouladi Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran
  • Leila Rostami Zadeh Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Ebrahim Sakhinia Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran; Tabriz Genetic Analysis Center (TGAC), Tabriz University of Medical Sciences, Tabriz, Iran

DOI:

https://doi.org/10.17305/bjbms.2017.1886

Keywords:

NFATc2, PPARG, colorectal cancer, gene expression, biomarker, CRC, targeted therapy

Abstract

Transcription factors are involved in cell cycle and apoptosis regulation and thus have a key role in the carcinogenesis of different tumors. Nuclear factor of activated T-cells, cytoplasmic 2 (NFATc2) and peroxisome proliferator-activated receptor gamma (PPARG) transcription factors are important in the carcinogenesis of colorectal cancer (CRC). In this study, we examined whether the expression of NFATc2 and PPARG genes is significantly altered during the carcinogenesis of CRC. A total of 47 tumor samples and matched normal tissue margins were collected during surgery from patients with CRC. In addition, three CRC cell lines (HCT119, SW480, and HT29) and healthy cell line were used. After total RNA extraction and cDNA synthesis, mRNA expression levels of NFATc2 and PPARG were examined by real-time polymerase chain reaction. The results showed that NFATc2 is overexpressed in the tumor tissues compared with normal tissue margins (p ≤ 0.05). However, the mRNA expression levels of PPARG were not significantly different between the tumor tissues and tissue margins. Our results indicate that NFATc2 may be used as an early diagnostic or predictive biomarker for CRC as well as a therapeutic target, providing that upcoming studies confirm these results.

Author Biographies

  • Venus Zafari, Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran
    Department of Biochemistry and Clinical Laboratories
  • Shahryar Hashemzadeh, Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran; General and Vascular Surgery Department of Tabriz University of Medical Sciences, Tabriz, Iran
    General and Vascular Surgery Department
  • Mohammadali Hosseinpour Feizi, Department of Animal Biology, University of Tabriz, Tabriz, Iran
    Department of Animal Biology
  • Nasser Pouladi, Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran

    Department of Biology

  • Leila Rostami Zadeh, Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
    Department of Biochemistry and Clinical Laboratories
  • Ebrahim Sakhinia, Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran; Tabriz Genetic Analysis Center (TGAC), Tabriz University of Medical Sciences, Tabriz, Iran
    Department of Biochemistry and Clinical Laboratories

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mRNA expression of nuclear factor of activated T-cells, cytoplasmic 2 (NFATc2) and peroxisome proliferator-activated receptor gamma (PPARG) transcription factors in colorectal carcinoma

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20-08-2017

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1.
mRNA expression of nuclear factor of activated T-cells, cytoplasmic 2 (NFATc2) and peroxisome proliferator-activated receptor gamma (PPARG) transcription factors in colorectal carcinoma. Biomol Biomed [Internet]. 2017 Aug. 20 [cited 2024 Mar. 28];17(3):255-61. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/1886