Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts

Authors

  • Anela Topcagic Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • Sanja Cavar Zeljkovic Central Laboratories and Research Support, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic; Department of Genetic Resources for Vegetables Medicinal and Special Plants, Centre of the Region Haná for Biotechnological and Agricultural Research, Crop Research Institute, Olomouc, Czech Republic
  • Erna Karalija Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • Semira Galijasevic Department of Medical Chemistry and Biochemistry, Sarajevo Medical School and Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina
  • Emin Sofic Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina; Department of Pharmacoinformatics and Pharmacoeconomics, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

DOI:

https://doi.org/10.17305/bjbms.2017.2049

Keywords:

Nigella sativa, UHPLC/MS-MS, phenolic compounds, butyrylcholinesterase inhibition, catalase inhibition, antimicrobial potential, black cumin

Abstract

Black cumin (Nigella sativa L. [N.sativa]) seed extracts demonstrated numerous beneficial biological effects including, among others, antidiabetic, anticancer, immunomodulatory, antimicrobial, anti-inflammatory, antihypertensive, and antioxidant activity. To better understand the phytochemical composition of N. sativa seeds, methanol seed extracts were analyzed for phenolic acid and flavonoid content. Furthermore, we tested N. sativa methanol, n-hexane, and aqueous seed extracts for their inhibitory activity against butyrylcholinesterase (BChE) and catalase (CAT) as well as for antimicrobial activity against several bacterial and a yeast strains. The phenolic content of N. sativa was analyzed using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The inhibition of BChE was assessed by modified Ellman’s method, and the inhibition of CAT was determined by monitoring hydrogen peroxide consumption. The extracts were tested against Bacillus subtilis, Staphylococcus aureus, Salmonella enterica, and Escherichia coli using the agar diffusion method. The UHPLC-MS/MS method allowed the identification and quantification of 23 phenolic compounds within 15 minutes. The major components found in N. sativa seed extract were sinapinic acid (7.22 ± 0.73 µg/mg) as a phenolic acid and kaempferol (11.74 ± 0.92 µg/mg) as a flavonoid. All extracts showed inhibitory activity against BChE, with methanol seed extract demonstrating the highest inhibitory activity (inhibitory concentration 50% [IC50] 79.11 ± 6.06 µg/ml). The methanol seed extract also showed strong inhibitory activity against CAT with an IC50 value of 6.61 ± 0.27 µg/ml. Finally, the methanol extract exhibited considerable inhibitory activity against the tested microbial strains. Overall, this is the first study to investigate the ability of black cumin seed extracts to inhibit CAT. Our results indicate that N. sativa seed can be considered as an effective inhibitor of CAT activity.

Author Biographies

  • Anela Topcagic, Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
    Department of Chemistry
  • Sanja Cavar Zeljkovic, Central Laboratories and Research Support, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic; Department of Genetic Resources for Vegetables Medicinal and Special Plants, Centre of the Region Haná for Biotechnological and Agricultural Research, Crop Research Institute, Olomouc, Czech Republic
    Department of Genetic Resources for Vegetables Medicinal and Special Plants
  • Erna Karalija, Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
    Department of Biology
  • Semira Galijasevic, Department of Medical Chemistry and Biochemistry, Sarajevo Medical School and Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina
    Department of Medical Chemistry and Biochemistry
  • Emin Sofic, Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina; Department of Pharmacoinformatics and Pharmacoeconomics, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

    Department of Chemistry;

    Department of Pharmacoinformatics and Pharmacoeconomics

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Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts

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20-11-2017

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1.
Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts. Biomol Biomed [Internet]. 2017 Nov. 20 [cited 2024 Mar. 28];17(4):286-94. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2049