Chromogranin A Detection in Saliva of Type 2 Diabetes Patients

  • Martine Soell Department of Periodontology, Hautepierre Hospitals, University of Strasbourg INSERM Unit 977
  • Ahmed Feki Department of Oral Medicine and Oral Surgery, University Hospitals
  • Matthias Hannig Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospitals, Saarland University
  • Hidehiko Sano Department of Restorative Dentistry, Division of Oral Health Science, Hokkaido University Graduate School of Dentistry
  • Michel Pinget Pôle NUDE, Medicale B, Department of Endocrinology, Diabetes and Metabolic Diseases, University Hospitals
  • Denis Selimović INSERM Unit 977 Department of Oral Medicine and Oral Surgery, University Hospitals
Keywords: Chromogranin A, diabetes type 2, innate immunity saliva

Abstract

Chromogranin A is present in secretion granules of nerve, endocrine and immune cells and is a precursor of several peptides with antibacterial and antifungal properties at micromolar concentrations.

Our aim in this prospective, double blind study, was to determine the expression of chromogranin A and its peptides at protein level in saliva of type 2 diabetic patients and thereby to obtain a new non-invasive diagnostic means for the future.

Saliva was taken from 30 type 2 diabetic patients and 30 healthy individuals at the same time interval in the morning without any oral stimuli. Circadianic periodics in protein productions have been avoided. The presence of chromogranin A and its derived peptides was determined in whole saliva, after centrifugation at 4°C for 12 min at 14 000 rpm, by SDS-PAGE electrophoresis and Immunoblotting (Western Blot). To ensure same protein concentrations Bradford protein quantification assay has been performed before.

For the first time, we have determined an overexpression of chromogranin A in saliva of diabetic patients in 100% of the individuals.

Chromogranin A, a circulating biomarker for epithelial tumours, is also overexpressed in saliva of type 2 diabetic patients. To confirm our results, more studies with a large amount of patients is necessary.

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Published
2010-02-20
How to Cite
1.
Soell M, Feki A, Hannig M, Sano H, Pinget M, Selimović D. Chromogranin A Detection in Saliva of Type 2 Diabetes Patients. Bosn J of Basic Med Sci [Internet]. 2010Feb.20 [cited 2019Nov.18];10(1):2-. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/2725
Section
Biochemistry