Pentoxifylline Ameliorates Glomerular Basement Membrane Ultrastructural Changes Caused by Gentamicin Administration in Rats

  • Nenad Stojiljković Department of Physiology, Faculty of Medicine, University of Niš
  • Slavimir Veljković Department of Physiology, Faculty of Medicine, University of Niš
  • Dragan Mihailović Department of Pathology, Faculty of Medicine, University of Niš
  • Milan Stoiljković Department of Pharmacology and Toxicology, Faculty of Medicine, University of Niš
  • Goran Ranković Department of Physiology, Faculty of Medicine, University of Niš
  • Ivan Jovanović Department of Anatomy, Faculty of Medicine, University of Niš
  • Pavle Randjelović Department of Physiology, Faculty of Medicine, University of Niš
Keywords: gentamicin, pentoxifylline, glomerular basement membrane, morphometry, rats

Abstract

Gentamicin is commonly used for the treatment of severe gram negative bacterial infections but inevi-tably cause renal failure during prolonged use. The aim of our study was to emphasize protective effects of pentoxifylline on glomerular basement membrane (GBM) alterations induced by gentamicin in rats. Experiments were done on 40 male Wistar rats divided in three experimental groups. GM-group was treated daily with gentamicin in dose of 100 mg/kg during 8 days. PTX-group was treated daily with pentoxifylline in dose of 45 mg/kg and the same dose of gentamicin as in GM-group during 8 days. The control group received 1 ml/day saline intraperitoneally. Morphometric parameter measured during the analysis was glomerular basement membrane thickness. In GM-group of animals glomeruli were en-larged and GMB was diffusely and unequally thickened with neutrophil cells infiltration. In proximal tu-bules epithelial cells, vacuolization of cytoplasm with coagulation-type necrosis were observed. In PTX-group of animals glomeruli were somewhat enlarged and GBM was thickened only in some segments. Coagulation-type necrosis was not found. Blood urea and serum creatinine concentration in GM-group were significantly elevated in comparison with PTX-group while potassium level was decreased. Our results suggest that PTX has protective effects on GBM and proximal tubules in GM-treated rats.

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Published
2009-08-20
How to Cite
1.
Stojiljković N, Veljković S, Mihailović D, Stoiljković M, Ranković G, Jovanović I, Randjelović P. Pentoxifylline Ameliorates Glomerular Basement Membrane Ultrastructural Changes Caused by Gentamicin Administration in Rats. Bosn J of Basic Med Sci [Internet]. 2009Aug.20 [cited 2019Apr.19];9(3):239-44. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/2814
Section
Pharmacology

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