Detection of Mycoplasma genitalium in female cervical samples by Multitarget Real-Time PCR

  • Sabina Mahmutović-Vranić Department of Microbiology, Faculty of Medicine, University of Sarajevo
  • Edina Bešlagić Department of Microbiology, Faculty of Medicine, University of Sarajevo
  • Justin Hardick Department of Infectious Diseases, School of Medicine, Johns Hopkins University
  • Maida Šlaković Institute for Health Protection of Women and Motherhood
  • Charlotte A. Gaydos Department of Infectious Diseases, School of Medicine, Johns Hopkins University
Keywords: Mycoplasmagenitalium, Multitarget real-time PCR

Abstract

Mycoplasma genitalum (MG) is associated with variety of urogenital infections such as non-gonococcal urethritis (NGU), endometritis and cervicitis. The objective of this study was to demonstrate and evaluate a research polymerase chain reaction (PCR) assay, for the detection of MG in cervical samples of a tested population of women attending gynecology clinics in Bosnia and Herzegovina. The Multitarget Real-Time (MTRT) PCR, utilizing the ABI 7900HT, the sequence detection system, was performed for the detection of MG. Cervical samples (N=97) from females were divided into three types of patient groups: Group 1: patients who had known abnormal clinical cytology reports (N=34); Group 2: patients who reported a history of genitourinary infections (N=22); and Group 3: patients not in either groups 1 or 2 (N=41). Overall, 14,43% (14/97) of those tested were positive for MG. A positive sample was defined as having a cycle threshold cross point (Ct) < 40,0 with a fluorescent detection comparable to the low positive control utilized during the run. This study validated the use of MTRT PCR as a reliable method for the detection of MG in clinical specimens and should facilitate large-scale screening for this organism.

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Published
2007-05-20
How to Cite
1.
Mahmutović-Vranić S, Bešlagić E, Hardick J, Šlaković M, A. Gaydos C. Detection of Mycoplasma genitalium in female cervical samples by Multitarget Real-Time PCR. Bosn J of Basic Med Sci [Internet]. 2007May20 [cited 2020Jul.13];7(2):109-12. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/3062
Section
Microbiology