Possible association of ABCB1:c.3435T>C polymorphism with high-density-lipoprotein-cholesterol response to statin treatment - a pilot study.

Anna Sałacka; Agnieszka Bińczak-Kuleta; Mariusz Kaczmarczyk; Iwona Hornowska; Krzysztof Safranow; Jeremy Simon Cabot Clark

doi:10.17305/bjbms.2014.3.43

Authors

Anna Sałacka Department of Family Medicine, Pomeranian Medical University, Szczecin, Poland
Agnieszka Bińczak-Kuleta Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Szczecin, Poland
Mariusz Kaczmarczyk Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Szczecin, Poland
Iwona Hornowska Department of Family Medicine, Pomeranian Medical University, Szczecin, Poland
Krzysztof Safranow Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Jeremy Simon Cabot Clark Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Szczecin, Poland

DOI:

https://doi.org/10.17305/bjbms.2014.3.43

Keywords:

ABCB1 transporter, HMG-CoA reductase inhibitors, P-glycoprotein

Abstract

The gene product ABCB1 (formerly MDR1 or P-glycoprotein) is hypothesized to be involved in cholesterol cellular trafficking, redistribution and intestinal re-absorption. Carriers of the ABCB1:3435T allele have previously been associated with decreases in ABCB1 mRNA and protein concentrations and have been correlated with changes in serum lipid concentrations. The aim of this study was to investigate possible association between the ABCB1:3435T>C polymorphism and changes in lipids in patients following statin treatment. Outpatients (n=130) were examined: 43 men (33%), 87 women (67%): treated with atorvastatin or simvastatin (all patients with equivalent dose of 20 or 40 mg/d simvastatin). Blood was taken for ABCB1:3435T>C genotyping, and before and after statin treatment for lipid concentration determination (total cholesterol, high-density-lipoprotein-cholesterol (HDL-C), triglycerides). Change (Δ) in lipid parameters, calculated as differences between measurements before and after treatment, were analyzed with multiple regression adjustments: gender, diabetes, age, body mass index, equivalent statin dose, length of treatment. Univariate and multivariate analyses showed significant differences in ΔHDL-C (univariate p=0.029; multivariate p=0.036) and %ΔHDL-C (univariate p=0.021; multivariate p=0.023) between patients with TT (-0.05 ± 0.13 g/l; -6.8% ± 20%; respectively) and CC+CT genotypes (0.004 ± 0.15 g/l; 4.1 ± 26%; respectively). Reduction of HDL-C in homozygous ABCB1:3435TT patients suggests this genotype could be associated with a reduction in the benefits of statin treatment.

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