Prevalence and genotype distribution of rotaviruses in children with gastroenteritis in Rize province

Selim Dereci; Ayşegül Çopur Çiçek; Sümeyra Savaş Acar; Zekiye Bakkaloğlu; Serdar Özkasap; Kadri Kanber; Şadan Hacisalihoğlu; Yücehan Albayrak; Rıza Durmaz

doi:10.17305/bjbms.2015.469

Authors

Selim Dereci Department of Pediatrics, School of Medicine Recep Tayyip Erdogan University Rize
Ayşegül Çopur Çiçek Department of Medical Microbiology, School of Medicine, Recep Tayyip Erdogan University Department of Pediatrics, Rize
Sümeyra Savaş Acar Turkey Public Health Agency, Research and Practice Laboratory of Molecular Microbiology, Ankara
Zekiye Bakkaloğlu Turkey Public Health Agency, Research and Practice Laboratory of Molecular Microbiology, Ankara
Serdar Özkasap Department of Pediatrics, School of Medicine, Recep Tayyip Erdogan University Department of Pediatrics, Rize
Kadri Kanber Department of Pediatrics, School of Medicine, Recep Tayyip Erdogan University Department of Pediatrics, Rize
Şadan Hacisalihoğlu Department of Pediatrics, School of Medicine, Recep Tayyip Erdogan University Department of Pediatrics, Rize
Yücehan Albayrak Department of Pediatrics, School of Medicine, Recep Tayyip Erdogan University Department of Pediatrics, Rize
Rıza Durmaz Turkey Public Health Agency, Research and Practice Laboratory of Molecular Microbiology, Ankara Yildirim Beyazıt University, Medicine Faculty, Department of Medical Microbiology, Ankara

DOI:

https://doi.org/10.17305/bjbms.2015.469

Keywords:

Rotaviruses, children, genotyping

Abstract

Determination of the distribution of rotavirus genotypes is essential for understanding the epidemiology of this virus responsible for nearly half a million of deaths in patients with gastroenteritis worldwide. In the present study, we aimed to genotype the rotavirus strains isolated from diarrheal stool samples in children under 5 years old. A total of 1297 fecal samples were collected, and rotavirus antigen was detected in 73 of these samples. Antigen-positive samples were transferred to the Public Health Agency of Turkey, Molecular Microbiology Research Laboratory, and were tested for determination of genotypes G and P using semi-nested multiplex polymerase chain reaction method performed with consensus- and genotype-specific primers. Twelve specimens were found to be negative for rotavirus in genotyping method. All the positive-strains were in G1-4, G8-9, P(4), P(8), and P(9) genotypes. The most frequent GP genotype combinations were found to be G9P(8) in 21 strains (34.4%), G2P(4) in 14 strains (23.0%), and G1P(8) in 12 strains (19.7%). We found 10 distinct genotypes amongst a total of 61 strains. Among the strains isolated and genotyped in our study, 90.2% (55/61) and 67.2% (41/61) have already been included in the two existing commercial vaccines. In conclusion, these findings implicate the necessity of development of region-specific vaccines after evaluation of the local genotype distribution. Further studies on the large number of rotavirus strains would contribute to this process.

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