2024-03-29T00:16:16Z
https://www.bjbms.org/ojs/index.php/bjbms/oai
oai:ojs2.www.bjbms.org:article/39
2021-03-17T15:36:42Z
bjbms:PAT
driver
Wound-healing potential of the fruit extract of Phaleria macrocarpa
Abood, Walaa Najm
Al-Henhena, Nawal Ahmed
Najim Abood, Ammar
Al-Obaidi, Mazen M. Jamil
Ismail, Salmah
Abdulla, Mahmood
Al Bartan, Rami
Antioxidant enzyme
inflammatory mediator
Phaleria macrocarpa
wound healing
The wound-healing potential of Phaleria macrocarpa was evaluated by monitoring the levels of inflammatory mediators, collagen, and antioxidant enzymes. Experimentally, two-centimeter-wide full-thickness-deep skin excision wounds were created on the posterior neck area of the rats. The wounds were topically treated with gum acacia as a vehicle in the control group, intrasite gel in the reference group, and 100 and 200 mg/mL P. macrocarpa fruit extract in the treatment group. Granulation tissues were excised on the 15th day and were further processed for histological and biochemical analyzes. Wound healing was evaluated by measuring the contractions and protein contents of the wounds. Cellular redistribution and collagen deposition were assessed morphologically using Masson’s trichrome stain. Superoxide dismutase (SOD) and catalase (CAT) activities, along with malondialdehyde (MDA) level were determined in skin tissue homogenates of the dermal wounds. Serum levels of transforming growth factor beta 1 (TGF-β1) and tumor necrosis factor alpha (TNF-α) were evaluated in all the animals. A significant decrease in wound area was caused by a significant increase in TGF-β1 level in the treated groups. Decrease in TNF-α level and increase in the collagen formation were also observed in the treated groups. Topical treatment with P. macrocarpa fruit extract increased the SOD and CAT activities in the healing wounds, thereby significantly increasing MDA level. The topical treatment with P. macrocarpa fruit extract showed significant healing effect on excision wounds and demonstrated an important role in the inflammation process by increasing antioxidant enzyme activities, thereby accelerating the wound healing process and reducing tissue injury.
Association of Basic Medical Sciences of FBIH
2015-05-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/39
10.17305/bjbms.2015.39
Biomolecules and Biomedicine; Vol. 15 No. 2 (2015); 25-30
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/39/124
https://www.bjbms.org/ojs/index.php/bjbms/article/view/39/2072
Copyright (c) 2014 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/44
2021-03-16T19:06:23Z
bjbms:PAT
driver
Stereological analysis of terminal villi of the placentas of pregnant woman with sideropenic anemia
Lelic, Melisa
Ramic, Suada
Zigic, Zlata
Bogdanovic, Gordana
Markovic, Sergije
placenta
terminal villi
pregnancy anemia
stereology
Iron deficiency, causing maternal sideropenic anemia, is one of the most frequent nutritive disorder that develops during the pregnancy. We collected 30 placentas from anemic mothers and 30 placentas from mothers belonging to the control group. Terminal villi (magnification 10x) and terminal villi capillaries (magnification 40x) were stereologically analyzed and numerically determined.
In the placentas from anemic mothers we noted the values a) terminal villi: volume density 0,43 mm0, surface density 24.13 mm-1, total volume 185.57 cm3 and total surface 10.27 m2; b) capillaries of terminal villi: volume density 0.53 mm0 and total volume 224.18 cm3. In the placentas from mothers belonging to the control group we observed the following values a) terminal villi: volume density 0.44 mm0, surface density 22.27 mm-1, total volume 200.17 cm3 and total surface 10.15 m2; b) capillaries of terminal villi: volume density 0.42 mm0 and total volume 197.00 cm3. Compared with the control group anemic mothers' placentas have a significant higher values of surface density of terminal villi (p<0.05), volume density (p <0.01) and absolute volume (p<0.0001) of terminal villi capillaries, and significant lower values of absolute volume of terminal villi (p<0.05).
In anemic mothers’ placentas, the total volume of terminal villi changes disproportionately to the total surface of terminal villi with statistically significant increase of terminal villi capillaries compared with control group.
Association of Basic Medical Sciences of FBIH
2014-08-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/139
10.17305/bjbms.2014.3.44
Biomolecules and Biomedicine; Vol. 14 No. 3 (2014); 139-143
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/139/43
https://www.bjbms.org/ojs/index.php/bjbms/article/view/139/2030
Copyright (c) 2015 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/175
2021-03-16T20:19:17Z
bjbms:PAT
driver
Secular trends in body height in Balkan populations from 1945 to 1995
Sarajlić, Nermin
Resić, Emina
Gradaščević, Anisa
Salihbegović, Adis
Balažic, Jože
Zupanc, Tomaž
Skeletonized remains
long bone lengths
mass grave
secular trend
Balkan populations
The aim of this study was to look for any secular trend in the stature of Balkan populations from the time of World War II (1939–1945) to the Balkans War (1991–1995). The research was based on the examination of exhumed skeletons of 202 men killed in World War II in the area of the Republic of Slovenia, and 243 men killed in the Bosnian War in Bosnia and Herzegovina. The length measurements of the right and left humerus, femur, tibia and fibula were taken. Since the results revealed no significant differences and the left-sided bones were more complete and recurrent in the sample, the bones of the left side were used in the analysis. Since the increase in height depends mostly on the increase in length of the long bones, with an average absolute change of about 0.28 cm for humerus, 0.55 cm for femur, 0.49 cm for tibia and 0.20 cm for fibula per decade in our case, these results suggest a significant increase of the height of the Balkans population. The difference of the sum of the average femur and tibia length for the study period was 4.13 cm. Recalculated average length increase of the sum length of femur and tibia per decade was 0.88 cm for the left side. Our study revealed that there was a trend towards increased long bone lengths, at least in the male population analyzed.
Association of Basic Medical Sciences of FBIH
2014-11-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/209
10.17305/bjbms.2014.4.175
Biomolecules and Biomedicine; Vol. 14 No. 4 (2014); 209-213
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/209/72
https://www.bjbms.org/ojs/index.php/bjbms/article/view/209/2044
Copyright (c) 2015 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/556
2021-03-01T17:57:09Z
bjbms:PAT
driver
Prognostic significance of survivin, β-catenin and p53 expression in urothelial carcinoma
Senol, Serkan
Yildirim, Asif
Ceyran, Bahar
Uruc, Fatih
Zemheri, Ebru
Ozkanli, Seyma
Akalin, Ibrahim
Ulus, Ismail
Caskurlu, Turhan
Aydin, Abdullah
Survivin
β-catenin
p53
bladder
urothelial carcinoma
clinicopathologic value
Survivin, β-catenin, and p53 are well-known cell-cycle and apoptosis regulators of tumorigenesis. Urothelial carcinomas (UCs) are the most common of the human cancers. Compared to superficial tumors (Ta, CIS, or T1), invasive UCs are important with regard to recurrence, progression, and mortality. Therefore, we examined whether survivin, β-catenin, and p53 could be used as the biomarkers for the early prediction of the invasiveness of UCs and the overall survival of the patients. We investigated the prognostic expressions of those biomarkers in UC (n=147) and in non-muscle invasive UC (NMI-UC) (n=113), using tissue microarray and immunohistochemistry. Spearman's correlation analysis and multivariate Cox regression analyses were used for statistical interpretation. High expressions of β-catenin, survivin, and p53 were associated with a high T stage, recurrence, progression, mortality, low recurrence-free survival, low progression-free survival and low overall survival (p < 0.01). Similar findings were achieved for recurrence and progression in the NMI-UC group, except for mortality. Moreover, a positive correlation was shown between p53 and β-catenin and between p53 and survivin (r=0.221, p < 0.01; r=0.236, p < 0.01, respectively). Survivin, p53, and β-catenin overexpression, as prognostic markers, might suggest that the UCs are biologically aggressive with the poor prognosis. Thus, dysregulation of those these cell-cycle and apoptosis regulators in bladder carcinoma could be used as a molecular marker to determine the best treatment strategy and could contribute to the development of targeted therapies.
Association of Basic Medical Sciences of FBIH
2015-08-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/556
10.17305/bjbms.2015.556
Biomolecules and Biomedicine; Vol. 15 No. 4 (2015); 7-14
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/556/162
https://www.bjbms.org/ojs/index.php/bjbms/article/view/556/1981
Copyright (c) 2015 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/619
2021-03-01T17:58:37Z
bjbms:PAT
driver
Correlation between advanced glycation end-products and the expression of fatty inflammatory factors in type II diabetic cardiomyopathy
Guo, Zhengdong
Huang, Donghui
Tang, Xiange
Han, Jingjing
Li, Jing
Cardiomyopathy
adiponectin
leptin
advanced glycation end-products
Diabetic cardiomyopathy (DCM) is one of the most severe complications of diabetes without a clear pathogenesis. Th is study investigated the adiponectin (APN) and leptin levels in type II DCM, as well as their correlation with advanced glycation end-products (AGEs). From 2011–2013, 78 type II diabetes mellitus (T2DM) cases (40–65 years old) in the Taian region were randomly selected. Based on the results of colour Doppler ultrasonography and coronary angiography, the cases were divided into a simple T2DM group (40 cases) and a DCM group (38 cases). Forty healthy subjects were used as normal control (NC). An enzyme-linked immunosorbent assay was performed to determine the levels of fa tty infl ammatory factors such as APN, leptin and AGEs, and a correlation analysis was conducted. In the T2DM group, the APN levels were decreased but the leptin and AGE levels were signifi cantly increased compared to the NC group. In the DCM group, the APN levels were decreased but the leptin and AGE levels were signifi cantly increased (P<0.01) compared to the T2DM group. Th e AGE levels were positively correlated with disease progression and with fasting plasma glucose levels, glycated haemoglobin, insulin resistance and leptin, but were negatively correlated with APN levels. Additionally, the APN and leptin levels were independently related to the AGE levels. Fatty infl ammatory factors play a signifi cant role in the progression of both simple T2DM and DCM. Th e results of this study revealed the pathogenesis of DCM and indicated the potential signifi cance of AGEs in DCM prevention and treatment.
Association of Basic Medical Sciences of FBIH
2015-10-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/619
10.17305/bjbms.2015.619
Biomolecules and Biomedicine; Vol. 15 No. 4 (2015); 15-19
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/619/181
https://www.bjbms.org/ojs/index.php/bjbms/article/view/619/1982
Copyright (c) 2015 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/638
2020-09-18T18:47:08Z
bjbms:PAT
driver
Influence of bone mineral density and hip geometry on the different types of hip fracture
Li, Yizhong
Lin, Jinkuang
Cai, Siqing
Yan, Lisheng
Pan, Yuancheng
Yao, Xuedong
Zhuang, Huafeng
Wang, Peiwen
Zeng, Yanjun
bone mineral density
hip
fragility fracture
femoral neck
The aim of this study was to assess the influence of bone mineral density and hip geometry on the fragility fracture of femoral neck and trochanteric region. There were 95 menopausal females of age ≥ 50 years with fragility fracture of hip, including 55 cases of femoral neck fracture and 40 cases of trochanteric fracture. Another 63 non-fractured females with normal bone mineral density (BMD) were chosen as control. BMD, hip axis length, neck-shaft angle and structural parameters including cross surface area, cortical thickness and buckling ratio were detected and compared. Compared with control group, the patients with femoral neck fracture or trochanteric fractures had significantly lower BMD of femoral neck, as well as lower cross surface area and cortical thickness and higher buckling ratio in femoral neck and trochanteric region. There were no significant differences of BMD and structural parameters in the femoral neck fracture group and intertrochanteric fracture group. Hip axis length and neck-shaft angle were not significantly different among three groups. The significant changes of BMD and proximal femur geometry were present in the fragility fracture of femoral neck and trochanteric region. The different types of hip fractures cannot be explained by these changes.
Association of Basic Medical Sciences of FBIH
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/638
10.17305/bjbms.2016.638
Biomolecules and Biomedicine; Vol. 16 No. 1 (2016); 35-38
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/638/198
https://www.bjbms.org/ojs/index.php/bjbms/article/view/638/1606
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/674
2020-09-18T18:45:18Z
bjbms:PAT
driver
The role of Six1 signaling in paclitaxel-dependent apoptosis in MCF-7 cell line
Armat, Marzieh
Oghabi Bakhshaiesh, Taiebeh
Sabzichi, Mehdi
Shanehbandi, Dariush
Sharifi, Simin
Molavi, Ommoleila
Mohammadian, Jamal
Saeid Hejazi, Mohammad
Samadi, Nasser
Six1
P53
apoptosis
MCF-7
paclitaxel-resistant cells
The resistance of cancer cells to chemotherapeutic agents represents the main problem in cancer treatment. Despite intensive research, mechanisms of resistance have not yet been fully elucidated. Six1 signaling has an important role in the expansion of progenitor cell populations during early embryogenesis. Six1 gene overexpression has been strongly associated with aggressiveness, invasiveness, and poor prognosis of different cancers. In this study, we investigated the role of Six1 signaling in resistance of MCF-7 breast cancer cells to taxanes. We first established in vitro paclitaxel-resistant MCF-7 breast cancer cells. Morphological modifications in paclitaxel-resistant cells were examined via light microscopic images and fluorescence-activated cell sorting analysis. Applying quantitative real-time polymerase chain reaction, we measured Six1, B-cell lymphoma/leukemia(BCL-2), BAX, and P53 mRNA expression levels in both non-resistant and resistant cells. Resistant cells were developed from the parent MCF-7 cells by applying increasing concentrations of paclitaxel up to 64 nM. The inhibitory concentration 50% value in resistant cells increased from 3.5 ± 0.03 to 511 ± 10.22 nM (p = 0.015). In paclitaxel-resistant cells, there was a significant increase in Six1 and BCL-2 mRNA levels (p = 0.0007) with a marked decrease in pro-apoptotic Bax mRNA expression level (p = 0.03); however, there was no significant change in P53 expression (p = 0.025). Our results suggest that identifying cancer patients with high Six1 expression and then inhibition of Six1 signaling can improve the efficiency of chemotherapeutic agents in the induction of apoptosis.
Association of Basic Medical Sciences of FBIH
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/674
10.17305/bjbms.2016.674
Biomolecules and Biomedicine; Vol. 16 No. 1 (2016); 28-34
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/674/190
https://www.bjbms.org/ojs/index.php/bjbms/article/view/674/1605
Copyright (c) 2015 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/721
2020-09-18T18:01:17Z
bjbms:PAT
driver
LGALS3 and AXIN1 gene variants playing role in the Wnt/ β-catenin signaling pathway are associated with mucinous component and tumor size in colorectal cancer
Korkmaz, Gurbet
Horozoglu, Cem
Arıkan, Soykan
Gural, Zeynep
Sağlam, Esra Kaytan
Turan, Saime
Özkan, Nazlı Ezgi
Kahraman, Ozlem Timirci
Yenilmez, Ezgi Nurdan
Düzköylü, Yigit
Doğan, Mehmet Baki
Zeybek, Umit
Ergen, Arzu
Yaylım, İlhan
Colorectal Cancer
LGALS3
AXIN1
ASO-PCR
PCR-RFLP
The Wnt pathway alterations have been identified in colorectal and many other cancer types. It has been reported that galectin-3 (which is encoded by the LGALS3 gene) alters the signaling mechanism in the Wnt/ β-catenin pathway by binding to β-catenin in colon and other cancers. AXIN1 is mainly responsible for the assembly of the β-catenin destruction complex in the Wnt pathway. This study investigated the relationship of rs4644 and rs4652 variants of the LGALS3 gene and rs214250 variants of the AXIN1 gene to histopathological and clinical properties. Our study included a total of 236 patients, of whom 119 had colorectal cancer (42 women, 77 men) and 117 were healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and allele-specific oligonucleotide (ASO) PCR methods were used. In addition, the serum galectin-3 level was studied with the enzyme-linked immunosorbent assay (ELISA) method. For the rs4644 variant of the LGALS3 gene, the CC genotype a mucinous component was significantly more common than those without a mucinous component (p=0.026). C allele frequency of the rs214250 variant of the AXIN1 gene was significantly correlated to tumor size in the advanced tumor stage (p=0.022). The CCAACT haplotype was more common in colorectal cancer patients (p=0.022). Serum galectin-3 level was higher in the patient group compared to the control group (5.9± 0.69 ng/ml vs. 0.79±0.01 ng/ml; p<0.001). In conclusion, variants of LGALS3 and AXIN1 genes affect tumor sizes and the mucinous component via Wnt/ β-catenin pathway in the pathogenesis of colorectal cancer.
Association of Basic Medical Sciences of FBIH
2016-02-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/721
10.17305/bjbms.2016.721
Biomolecules and Biomedicine; Vol. 16 No. 2 (2016); 108-113
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/721/223
https://www.bjbms.org/ojs/index.php/bjbms/article/view/721/1592
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1206
2021-02-17T22:12:31Z
bjbms:PAT
driver
Lithium chloride could aggravate brain injury caused by 3-nitropropionic acid
Milutinović, Aleksandra
Lithium chloride
3-nitropropionic acid
cytochrome c oxidase
synaptotagmin-4
synaptotagmin-7
Lithium, a well-known drug for the treatment of bipolar disorder, may also have the ability to reduce neurodegeneration and stimulate cell proliferation. Systemic injection of mitochondrial toxin 3-nitropropionic acid (3NPA) is known to induce a relatively selective, Huntington disease-like brain injury. The aim of this study was to determine the effect of lithium chloride (LiCl) on brain injury caused by 3NPA. Female adult Wistar rats were pre-treated with LiCl (127 mg/kg) 1 day before the first injection of 3NPA (28 mg/kg), and then for 8 days with the same treatment but receiving LiCl 1 hour before 3NPA. Control groups were pre-treated accordingly, with LiCl or with normal saline, but were not treated with 3NPA. Staining for cytochrome c oxidase activity and in situ hybridization autoradiography of synaptotagmin-4 and -7 mRNAs were used to evaluate brain injury caused by 3NPA. There was a significant reduction of body weight in the 3NPA+LiCl group (79%) compared to the 3NPA group (90%, p = 0.031) and both control groups (100%, p = 0.000). Densitometric evaluation of cytochrome c oxidase staining and in situ hybridization autoradiograms revealed that the pre-treatment with LiCl caused an increase in striatal lesion for about 40% (p = 0.049). Moreover, the lesion was observed also in the hippocampus of three animals from the 3NPA+LiCl group and in two animals from the 3NPA group. However, there were no differences between the LiCl and saline group in any of the measured parameters. We concluded that the pre-treatment with a relatively nontoxic dose of LiCl could aggravate brain injury caused by 3NPA.
Association of Basic Medical Sciences of FBIH
2016-11-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1206
10.17305/bjbms.2016.1206
Biomolecules and Biomedicine; Vol. 16 No. 4 (2016); 261-267
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1206/253
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1206/1942
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1209
2021-02-17T22:13:08Z
bjbms:PAT
driver
Differential expression of androgen, estrogen, and progesterone receptors in benign prostatic hyperplasia
Song, Lingmin
Shen, Wenhao
Zhang, Heng
Wang, Qiwu
Wang, Yongquan
Zhou, Zhansong
Benign prostatic hyperplasia
androgen receptor
estrogen receptor α
estrogen receptor β
progesterone receptor
rat model
This study aimed to identify the differential expression levels of androgen receptor (AR), estrogen receptors (ERα, ERβ), and progesterone receptor (PGR) between normal prostate and benign prostatic hyperplasia (BPH). The combination of immunohistochemistry, quantitative real-time reverse transcription polymerase chain reaction, and Western blotting assay was used to identify the distribution and differential expression of these receptors at the immunoactive biomarker, transcriptional, and protein levels between 5 normal human prostate tissues and 40 BPH tissues. The results were then validated in a rat model of BPH induced by testosterone propionate and estradiol benzoate. In both human and rat prostate tissues, AR was localized mainly to epithelial and stromal cell nuclei; ERα was distributed mainly to stromal cells, but not exclusively; ERβ was interspersed in the basal layer of epithelium, but sporadically in epithelial and stromal cells; PGR was expressed abundantly in cytoplasm of epithelial and stromal cells. There were decreased expression of ERα and increased expression of PGR, but no difference in the expression of ERβ in the BPH compared to the normal prostate of both human and rat. Increased expression of AR in the BPH compared to the normal prostate of human was observed, however, the expression of AR in the rat prostate tissue was decreased. This study identified the activation of AR and PGR and repression of ERα in BPH, which indicate a promoting role of AR and PGR and an inhibitory role of ERα in the pathogenesis of BPH.
Association of Basic Medical Sciences of FBIH
2016-07-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1209
10.17305/bjbms.2016.1209
Biomolecules and Biomedicine; Vol. 16 No. 3 (2016); 201-208
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1209/254
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1209/1958
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1233
2021-02-17T22:08:00Z
bjbms:PAT
driver
Significance of Foxp3+CD4+ regulatory T cells in the peripheral blood of Uygur patients in the acute and chronic phases of pigeon breeder’s lung
Yu, Biqing
Yang, Xiaohong
Li, Fengsen
Wu, Chao
Wang, Wenyi
Ding, Wei
Pigeon breeder’s lung
Foxp3 CD4 Treg
T lymphocyte
Uygur
Pigeon breeder’s lung (PBL) is a type of lung inflammatory disease associated with the immune response to repeated pigeon-derived antigen exposure. The pathogenesis of PBL remains unclear. In this study, peripheral blood samples were collected from Uygur acute - and chronic-phase PBL patients and healthy subjects with pigeon contact. Foxp3+CD4+ regulatory T cell (Treg) activity in different phases of PBL was characterized by changes in Foxp3+CD4+ Treg, CD4+CD25+ T cell, and T lymphocyte subsets. Based on hypersensitivity pneumonitis (HP) diagnosis criteria, 32 PBL cases from January 2012 to December 2013 in the People’s Hospital of Xinjiang Uygur Autonomous Region Respiratory Department were included. Lung high-resolution computed tomography was performed, and the cases were classified based on the HP phase into 15 acute-phase and 17 chronic-phase cases. The control group included 30 healthy subjects with Uygur pigeon contact. Blood samples were collected, and the T cell subsets were analyzed via flow cytometry. In both PBL groups, the Foxp3+CD4+ Treg and CD4+CD25+ and CD4+CD3+ T cell percentages and CD4+/CD8+ ratios were significantly lower than in the control group (p < 0.01). In the PBL groups, particularly the acute-phase group, the CD8+CD3+ T lymphocyte percentage was significantly higher than in the control group (p < 0.01). There were no significant differences in CD4+CD25+ cells between the PBL groups. In peripheral blood from the PBL groups, the CD4+/CD8+ ratio was positively correlated with the Foxp3+CD4+ Treg (r = 0.864, p < 0.05) and CD4+/CD25+ cell (r = 0.34, p < 0.05) percentages. Low Foxp3+CD4+ Treg expression or overconsumption may be a pathogenic factor in PBL.
Association of Basic Medical Sciences of FBIH
2017-02-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1233
10.17305/bjbms.2016.1233
Biomolecules and Biomedicine; Vol. 17 No. 1 (2017); 17-22
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1233/275
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1233/1930
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1238
2021-02-17T22:12:27Z
bjbms:PAT
driver
Microsatellite instability and B-type Raf proto-oncogene mutation in colorectal cancer: Clinicopathological characteristics and effects on survival
Batur, Sebnem
Vuralli Bakkaloglu, Dogu
Kepil, Nuray
Erdamar, Sibel
Microsatellite instability
DNA mismatch repair genes
B-type Raf proto-oncogene mutation
survival
polymerase chain reaction
immunohistochemistry
Prognostic significance of microsatellite instability (MSI) status and B-type Raf proto-oncogene (BRAF) mutation in colorectal cancer is controversial. The aim of this study was to examine the clinical and pathological characteristics associated with microsatellite stability and the effect of MSI and BRAF mutation on the survival of patients with colorectal cancer. The study included 145 colorectal cancer cases. All the patients were examined for DNA mismatch repair (MMR) proteins with an immunohistochemical method. Molecular assessment of MSI was available in a subset of 41 patients. In addition, BRAF mutation analysis was performed in 30 cases. Immunohistochemically, MMR deficiency was present in 28 (19.3%) patients. Female gender (p = 0.001), lesion size ≥5 cm (p = 0.013), Crohn-like response (p = 0.035), and right-sided localization (p < 0.001) were significantly more frequent among MMR-deficient patients. The overall survival was 44.1 ± 5.1 months (95% confidence interval [CI], 33.7-54.4). Multivariate analyses identified only high tumor grade as an independent predictor of poor overall survival: odd ratio, 6.7 (95% CI 2.1-21.7), p = 0.002. In the subset of patients with available BRAF assessment (n = 30), a negative BRAF status was associated with better survival when compared to a positive BRAF status (36.7 ± 2.1 vs. 34.1 ± 7.2 months, p = 0.048). The sensitivity and specificity of the immunohistochemical method in predicting positive MSI status, with the molecular method as a reference, were 85.7% (95% CI: 56.2%-97.5%) and 88.9% (95% CI: 69.7%-97.1%), respectively. BRAF appears to be a significant predictor of a worse outcome in patients with colorectal cancer. Further studies with a large spectrum of clinical and biological variables are warranted.
Association of Basic Medical Sciences of FBIH
2016-11-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1238
10.17305/bjbms.2016.1238
Biomolecules and Biomedicine; Vol. 16 No. 4 (2016); 254-260
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1238/247
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1238/1941
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1497
2021-02-04T21:22:30Z
bjbms:PAT
driver
Human epidermal growth factor receptor 2 (HER-2) status evaluation in advanced gastric cancer using immunohistochemistry versus silver in situ hybridization
Kepil, Nuray
Batur, Sebnem
Sonmez Wetherilt, Ceyda
Erdamar Cetin, Sibel
Human epidermal growth factor receptor 2
gastric cancer
immunohistochemistry
silver in situ hybridization
Accurate identification of human epidermal growth factor receptor 2 (HER-2) status in advanced gastric cancer patients is of utmost importance in terms of treatment planning. This study aimed to examine the HER-2 status in advanced gastric cancer patients using both immunohistochemistry (IHC) and silver in situ hybridization (SISH) techniques and to investigate concordance and diagnostic accuracy. In addition, associations between clinical parameters and HER 2 status were examined. A total of 313 patients diagnosed with locally advanced (Stage III: T3-4, N+) recurrent or metastatic adenocarcinoma of the stomach or esophagogastric junction, between 2009 and 2015, were included. HER-2 status was examined using both IHC and SISH techniques and the findings were compared. Overall SISH-confirmed HER-2 positivity rate was 22%. Multivariate analysis identified only well-differentiated tumor as a significant predictor of HER-2 positivity (OR: 2.9, 95% CI: 1.4-5.9, p = 0.003). When IHC 2+ and 3+ were considered positive for HER-2 status, sensitivity, specificity, and concordance rate (κ) was 95.7%, 93.8%, and 0.84, respectively. Corresponding figures when only IHC 3+ cases were considered positive were lower: 50%, 100%, and 0.61, respectively. The present method used for the identification of HER-2 positive gastric cancer patients provides satisfactory results. However, better categorization of IHC 2+ cases has the potential to improve the diagnostic accuracy, which is particularly important when more sophisticated methods are not readily available.
Association of Basic Medical Sciences of FBIH
2017-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1497
10.17305/bjbms.2016.1497
Biomolecules and Biomedicine; Vol. 17 No. 2 (2017); 109-113
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1497/278
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1497/1911
Copyright (c) 2016 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1696
2021-02-04T21:17:53Z
bjbms:PAT
driver
Defective autophagy is associated with neuronal injury in a mouse model of multiple sclerosis
Feng, Xuedan
Hou, Huiqing
Zou, Yueli
Guo, Li
Autophagy
experimental autoimmune encephalomyelitis
multiple sclerosis
neurodegeneration
apoptosis
EAE mice
LC3-II
Beclin1
rapamycin
3-methyladenine
p62
autophagy deficiency
Neurodegeneration, along with inflammatory demyelination, is an important component of multiple sclerosis (MS) pathogenesis. Autophagy is known to play a pivotal role in neuronal homeostasis and is implicated in several neurodegenerative disorders. However, whether autophagy is involved in the mechanisms of neuronal damage during MS remains to be investigated. Experimental autoimmune encephalomyelitis (EAE), an in vivo model of MS, was induced in female C57BL/6 mice by immunization with myelin oligodendrocyte glycoprotein p35-55. After that, autophagic flux in the spinal cord of mice was evaluated by detection of LC3-II and Beclin1 protein expressions. EAE mice were then administered with rapamycin and 3-methyladenine (3-MA) for 10 days. Afterward, the changes in LC3-II, Beclin1, and p62 expression, number of infiltrated inflammatory cells, demyelinated lesion area, and neuronal damage, as well as clinical scores, were assessed. Further, apoptotic cell rate and apoptosis-related protein expressions were monitored. We observed an impaired autophagic flux and increased neuronal damage in the spinal cords of EAE mice. We also found that rapamycin, an autophagy inducer, mitigated EAE-induced autophagy decrease, inflammation, demyelination and neuronal injury, as well as the abnormal clinical score. In addition, rapamycin suppressed cell apoptosis, and decreased Bax/Bcl-2 ratio and cleaved caspase-3 expression. Conversely, the effect of autophagy inhibitor 3-MA on EAE mice resulted in completely opposite results. These results indicated that autophagy deficiency, at least in part, contributed to EAE-induced neuronal injury and that pharmacological modulation of autophagy might be a therapeutic strategy for MS.
Association of Basic Medical Sciences of FBIH
2017-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1696
10.17305/bjbms.2017.1696
Biomolecules and Biomedicine; Vol. 17 No. 2 (2017); 95-103
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1696/293
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1696/1909
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1715
2021-02-11T20:06:21Z
bjbms:PAT
driver
Dose- and time-dependent effects of clodronate on orthodontic tooth movement
Nakaš, Enita
Lauc, Tomislav
Tiro, Alisa
Džemidžić, Vildana
Zukanović, Amila
Franić, Miljenko
Ivković, Vladimir
Histology
tooth movement
pharmacology
bone biology
clodronate
bisphosphonate
Orthodontic tooth movement is the result of bone remodeling that occurs in periodontal ligament and alveolar bone tissue as a response to mechanical loading of the tooth. The aim of this study is to investigate the time- and dose-response effects of locally administered clodronate on tooth movement. Sixty Wistar rats were randomly assigned to 4 groups of 15 specimens: E1 - application of 10 mMol of clodronate in 3-day intervals; E2 - application of 2.5 mMol of clodronate in 3-day intervals; E3 - application of 10 mMol of clodronate in 7-day intervals; E4 - application of 2.5 mMol of clodronate in 7-day intervals. A 50 μL clodronate solution was injected into a subperiosteal area to the right maxillary incisor. The left maxillary incisor served as a control, with an injection of saline solution. In 3-day interval application regime, there was no effect of clodronate dosing on tooth movement. In 7-day interval application regime, decreased tooth movement was observed with 10 mMol compared with 2.5 mMol clodronate concentration. However, decreased tooth movement was also observed when 2.5 mMol of clodronate was applied in 7-versus 3-day intervals. Conversely, no difference was observed when 10 mMol concentration was applied in 3- versus 7-day intervals. When clodronate is applied subperiosteally in the root area, it decreases the tooth movement. Tooth movement is impeded by the higher clodronate dosing, as well as by shorter application interval even with lower dosing. The purpose of future trials should, therefore, be to determine a safe therapeutic dose/interval application of clodronate in humans and their potential side effects.
Association of Basic Medical Sciences of FBIH
2017-02-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1715
10.17305/bjbms.2017.1715
Biomolecules and Biomedicine; Vol. 17 No. 1 (2017); 23-28
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1715/294
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1715/1931
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1750
2021-02-04T21:20:17Z
bjbms:PAT
driver
High Ki-67 expression is associated with prolactin secreting pituitary adenomas
Bălinişteanu, Bogdan
Cîmpean, Anca Maria
Ceauşu, Amalia Raluca
Corlan, Ana Silvia
Melnic, Eugen
Raica, Marius
Pituitary adenomas
Ki-67 labeling index
prognostic factor
prolactin
PRL
PRL-secreting adenoma
Pituitary adenomas represent the third most common primary intracranial tumor in neurosurgical practice. To understand the biological behaviour of the pituitary adenomas previous studies have determined the tumor proliferation rate using monoclonal antibodies targeted against the Ki-67 antigen. The aim of this study was to correlate the Ki-67 index with hormonal profiles of pituitary adenomas. The study included 50 pituitary adenomas. For histopathologic evaluation, the sections were stained with routine hematoxylin and eosin method. Additional paraffin sections from each tumor were immunostained using primary antibodies against the following pituitary hormones: somatotropin (STH), prolactin (PRL), adrenocorticotrophic hormone (ACTH), thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), and luteinizing hormone (LH). To detect the expression of Ki-67 we used a mouse anti-human monoclonal antibody (clone K2). The percentage of Ki-67 positive nuclei (Ki-67 labeling index) was assessed by counting approximately 1000 nuclei of the tumor cells at ×400 magnification. Out of the 50 tumor samples, 31 (62%) pituitary adenomas showed proliferative activity, and the proliferation rate was variable in this group. The overall mean Ki-67 labeling index was 1.59 ± 1.47, ranging from 0.3% to 6.6%. In 5 cases, the Ki-67 index was >3%, all of them being prolactinomas. The Ki-67 index was higher in PRL-secreting adenomas (mean ± SD was 3.37 ± 1.80, range 0.9 - 6.6%). Our study provides the evidence that a higher Ki-67 value is associated with pituitary adenomas that secrete PRL (prolactinomas and mixed STH/PRL-secreting adenomas).
Association of Basic Medical Sciences of FBIH
2017-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1750
10.17305/bjbms.2017.1750
Biomolecules and Biomedicine; Vol. 17 No. 2 (2017); 104-108
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1750/312
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1750/1910
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/1938
2021-02-17T22:09:33Z
bjbms:PAT
driver
Enhancer of zeste homologue 2 (EZH2) expression in synovial sarcomas as a promising indicator of prognosis
Yalçınkaya, Ulviye
Uğraş, Nesrin
Özgün, Gonca
Ocakoğlu, Gökhan
Deligönül, Adem
Çetintaş, Sibel Kahraman
Bilgen, Muhammed Sadık
Enhancer of zeste homologue 2 (EZH2)
immunohistochemistry
prognostic factors
synovial sarcoma
survival analysis
Synovial sarcoma (SS) is a type of soft-tissue sarcoma, often linked to poor survival. Although overexpression of enhancer of zeste homologue 2 (EZH2) has been associated with poor prognosis in different tumors, a few studies investigated this link in SS. Here, we analyzed the relationship between EZH2 expression and prognostic factors in SS. We included 29 patients with SS. Immunostaining of EZH2 was performed with (D2C9) XPTM Rabbit mAb antibody, and the results were classified as low EZH2 expression (negative or weak expression) and high EZH2 expression category (moderate or strong expression). Analysis of survival in relation to prognostic factors was performed with Kaplan-Meier survival curves and Cox proportional hazard regression analysis. Our sample included 19/29 female and 10/29 male patients, with age range 16-63 years. The tumor diameter ranged from 2 to 15 cm. Necrosis was observed in 15/29 cases. Sixteen cases had >10 mitoses per 50 high-power fields (HPFs). Out of 29 cases, 14 showed low and 15 had high EZH2 expression. Statistically significant results were obtained for the association between the presence of metastasis and necrosis (p = 0.042), high EZH2 expression and distant metastasis (p = 0.018), high EZH2 expression and necrosis (p = 0.016), and high EZH2 expression and the tumor size >5 cm versus tumor size ≤5 cm (p = 0.014). Patients with all of the following: the tumor size ≤5 cm, low EZH2 expression, and without necrosis and distant metastasis had significantly longer survival time. Our results are consistent with previous studies, suggesting that EZH2 overexpression is an indicator of poor prognosis in SS.
Association of Basic Medical Sciences of FBIH
2017-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1938
10.17305/bjbms.2017.1938
Biomolecules and Biomedicine; Vol. 17 No. 4 (2017); 302-308
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1938/358
https://www.bjbms.org/ojs/index.php/bjbms/article/view/1938/1873
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2039
2021-01-17T20:50:53Z
bjbms:PAT
driver
Follicular morphological characteristics may be associated with invasion in follicular thyroid neoplasms with papillary-like nuclear features
Can, Nuray
Celik, Mehmet
Sezer, Yavuz Atakan
Ozyilmaz, Filiz
Ayturk, Semra
Tastekin, Ebru
Sut, Necdet
Gurkan, Hakan
Ustun, Funda
Bulbul, Buket Yilmaz
Guldiken, Sibel
Puyan, Fulya Oz
Noninvasive follicular thyroid neoplasm with papillary-like nuclear features
NIFTP
EFVPTC
histomorphological features
follicular variant of papillary thyroid carcinoma
FVPTC
NRAS
BRAF
mutation
The newly proposed nomenclature and diagnostic criteria for encapsulated follicular variant of papillary thyroid carcinoma (EFVPTC), the noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP), could improve the consistency and accuracy of diagnosing this entity. Diagnosis of NIFTP requires evaluation of the complete tumor border or capsule. The presence of tumor invasion in follicular thyroid neoplasms with papillary-like nuclear features has been recently discussed by many authors. In this study, we examined the predictive value and association of follicular morphological characteristics with the tumor invasion. In addition, we analyzed the association between tumor encapsulation and molecular profile in EFVPTC/NIFTP cases. A total of 106 cases of FVPTC were included in the study. The tumors were grouped based on the presence of tumor capsule and characteristics of tumor border, as 1) completely encapsulated tumors without invasion, 2) encapsulated tumors with invasion, 3) infiltrative tumors without a capsule. Clinicopathological features, histomorphological features [nuclear criteria, minor diagnostic features, follicles oriented perpendicular to tumor border/capsule (FOPBC)] and molecular alterations in BRAF, NRAS, and KRAS genes were evaluated. FOPBC were significantly more frequently seen in encapsulated tumors with invasion (p = 0.008). The nuclear features were not associated with the presence of encapsulation and characteristics of tumor border. BRAF mutation was more frequent in infiltrative tumors, while NRAS mutation was more frequent in encapsulated tumors, but the results were not statistically significant (p = 0.917). In conclusion, FOPBC histomorphological feature may be associated with tumor invasion in EFVPTC/NIFTP. Additionally, BRAF/KRAS/NRAS mutation analysis may prevent inadequate treatment in these patients.
Association of Basic Medical Sciences of FBIH
2017-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2039
10.17305/bjbms.2017.2039
Biomolecules and Biomedicine; Vol. 17 No. 3 (2017); 211-220
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2039/314
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2039/1860
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2293
2021-03-16T17:33:16Z
bjbms:PAT
driver
A histological study of the effect of exogenous melatonin on gentamicin induced structural alterations of proximal tubules in rats
Kapić, Dina
Mornjaković, Zakira
Ćosović, Esad
Šahinović, Maida
gentamicin
melatonin
proximal tubules
rats
histological analysis
The aim of this research was to assess the reactive changes of rat proximal tubules caused by gentamicin and the effect of relatively low doses of melatonin. 48 adult male Wistar rats were distributed into six groups of equal size which all received one of the following daily intraperitoneal injections: vehicle (5% ethanol in Ringer solution) during 11 days (C); gentamicin (80 mg/kg) during 8 days (G), two groups which concomitantly received gentamicin (80 mg/kg) during 8 days and melatonin in two different test doses (5 or 20 mg/kg) during 11 days (GM1, GM2) and two groups treated only with melatonin in two different doses (5 or 20 mg/kg) during 11 days (M1, M2). Histological analysis included qualitative and semi-quantitative light microscopy analysis of proximal tubules. Exogenous melatonin had no significant effect on the microstructure, independently of dosis. The changes of proximal tubules microstructure induced by gentamicin were expressed in the form of granulovacuolar degeneration, necrosis and desquamation. The grade of proximal tubular changes was smaller in animals who besides gentamicin received melatonin. Melatonin has a dose dependent protective effect on the structural alterations of proximal tubules of the kidney induced by gentamicin.
Association of Basic Medical Sciences of FBIH
2014-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2293
10.17305/bjbms.2014.2293
Biomolecules and Biomedicine; Vol. 14 No. 1 (2014); 30-34
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2293/349
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2293/2006
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2348
2021-05-04T22:29:50Z
bjbms:PAT
driver
Pathologic morphology of acute experimental osteomyelitis
S. Strelkov, Nikolay
A. Kiryanov, Nikolay
O. Shklyaev, Pavel
M. Iryanov, Yury
osteomyelitis
experiment
early stages
osteoperforation
The goal of our work was to study the changes in the bone tissue, bone marrow and surrounding tissues in animals during early stages of experimental osteomyelitis. Osteomyelitis was simulated in 30 infants rabbits aged 3-4 months whose body weight accounted 1200-1600 grams through the insertion of 5-6 million of aurococcus into the marrow channel of a shinbone. The study of bone marrow, bone tissue and surrounding tissue was conducted 30 minutes, 6, 12, 24, 48 and 60 hours after the contamination with the help of light and electronic (transmission and scanning) microscopy. It was proved that the first changes are characterized by the evident changes in the vessel’s walls which cause the swelling of bone marrow and suppurative inflammation in the bone tissue occurs only in the end of the 3d day of the experiment. These data confirm the necessity of osteoperfortation during the first 24 hours of contamination in order to remove the swelling and to correct vessel disorders.
Association of Basic Medical Sciences of FBIH
2013-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2348
10.17305/bjbms.2013.2348
Biomolecules and Biomedicine; Vol. 13 No. 3 (2013); 153-157
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2348/375
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2348/2136
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2357
2021-05-04T22:31:23Z
bjbms:PAT
driver
Kidney injury molecule-1 expression is closely associated with renal allograft damage
Song, Lianlian
Xue, Lijuan
Yu, Jinyu
Zhao, Jun
Zhang, Wenlan
Fu, Yaowen
kidney injury molecule-1
renal allograft
biomarker
The aim of our study was to investigate the expression of kidney injury molecule-1 (KIM-1) in renal allograft biopsy samples and assess the clinical significance of its use as a biomarker for tissue damage. A total of 69 renal allograft biopsy samples from 17 patients with normal serum creatinine and 52 cases of increased serum creatinine were collected. They were divided into different groups according to the Banff 2007 diagnostic criteria. KIM-1 expression was detected by immunohistochemical methods and the association of KIM-1 and blood biochemical indexes was analyzed. KIM-1 expression increased as Banff 2007 classification grade increased and was positively correlated with tubular inflammation severity in the acute T-cell rejection group. Moreover, KIM-1 expression was strongly positive in the chronic active antibody-mediated rejection group. Interestingly, KIM-1 was weakly positive in the normal group without obvious acute rejection and injury of immunosuppressant toxicity. In this group, 27.3% (3/11) of the cases with normal serum creatinine level showed weakly positive KIM-1 expression in their renal tissues. KIM-1 expression level is positively correlated with renal allograft damage and tubular cell injury. KIM-1 is expressed in tubular epithelial cells before blood biochemical indexes become elevated and morphological changes occur. KIM-1 expression is an early, sensitive, and specific biomarker to determine renal tubular epithelial cell injury in renal allograft tissue.
Association of Basic Medical Sciences of FBIH
2013-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2357
10.17305/bjbms.2013.2357
Biomolecules and Biomedicine; Vol. 13 No. 3 (2013); 170-174
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2357/378
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2357/2137
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2368
2021-05-04T23:28:13Z
bjbms:PAT
driver
Immunohistochemical expression and significance of NM23 suppressor protein in primary gastric adenocarcinoma
Radović, Svjetlana
Dorić, Mirsad
Hukić, Ajna
Babić, Mirsad
Kuskunović, Suada
Spahović, Naida
gastric cancer
NM23
immunohistochemistry
NM 23 protein was originally identified as a metastasis suppressor protein. The expression of NM23 has been correlated with tumour metastatic potential in various human carcinoma, mostly in ductal breast and colorectal carcinomas. Evidence for their expression in gastric cancer is rather contradictory, both for protein expression status and prognostic vale. This study was done to analyze the immunohistochemical expression of NM23 in gastric carcinoma, and correlation of the degree of staining with clinicopathological parameters was investigated.
In a retrospective immunohistochemical study specimens obtained from 56 gastric cancer patients who had undergone gastrectomy with perigastric lymphadenectomy were analysed, in correlation with classical clinical-pathological parameters of tumours, WHO-, Lauren-, Goseki-, and Ming- classification. NM 23 gene expression was compared in gastric adenocarcinoma and tumour-adjacent non-neoplastic gastric mucosa. A semiquantitative immunostaining evaluation (score 0-3) was used, counting the percentage of stained cells. Statistical analysis was performed using Kolmogorov-Smirnov test, and Spearman rank correlation test.
The investigated group consisted of 40 males and 16 females (2.5:1) with a mean age of 63 years (range: 48-81 years). The percentage of positive expression of NM23 (score 3) were in 30 (53.5%) specimens in non-neoplastic mucosa in adjacent gastric carcinoma, and negative (score 0-2) in all 56 (100%) specimens of gastric adenocarcinoma. NM23 expression was higher in non-neoplastic mucosa than in adjacent gastric adenocarcinoma tissue (p<0.0001). NM23 protein expression did not correlate with gender (p=0.115), tumour size (p=0.844), tumour grade (p>=0.172), lymphovascular invasion (p=0.606), lymph node metastases (p=0.311), Lauren classification (p=0.426), Goseki classification (p=0.458) and Ming classification (p=0.212).
Our series did not show a significant correlation between NM23 expression and analysed clinico-pathological variables, but these results suggest that protein NM23 may have a role in gastric carcinoma pathogenesis.
Association of Basic Medical Sciences of FBIH
2013-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2368
10.17305/bjbms.2013.2368
Biomolecules and Biomedicine; Vol. 13 No. 2 (2013); 72-77
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2368/387
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2368/2164
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2378
2021-01-09T14:01:57Z
bjbms:PAT
driver
Immunohistochemical expression of NEDD9, E-cadherin and γ-catenin and their prognostic significance in pancreatic ductal adenocarcinoma (PDAC)
Radulović, Petra
Krušlin, Božo
Pancreatic ductal adenocarcinoma
PDAC
NEDD9
E-cadherin
γ-catenin
immunohistochemistry
Extensive research is being conducted to identify novel diagnostic, predictive and prognostic biomarkers for pancreatic ductal adenocarcinoma (PDAC), as only a few markers have been routinely used so far with limited success. Our aim was to assess the expression of neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), E-cadherin, and γ-catenin in PDAC in relation to clinicopathological parameters and patient survival. We also investigated if there is a correlation of NEDD9 expression with E-cadherin or γ-catenin. The protein expression was determined by immunohistochemistry in 61 PDAC and 61 samples of normal pancreatic tissue. The log rank test and Kaplan-Meier survival curve were used for survival analysis. E-cadherin and γ-catenin expressions were reduced in PDAC, and completely retained in normal pancreatic tissue. Expression of NEDD9 was significantly increased in PDAC (strong expression in 78.7% of cases and moderate in 21.3%) and reduced in normal pancreatic tissue (strong positivity in 45.9% of cases, moderate in 31.1%, and weak in 23%). There was a positive correlation between reduced E-cadherin and γ-catenin expression in PDAC (p = 0.015). The loss or reduced expression of E-cadherin had a negative impact on patient survival (p = 0.020). A negative correlation between E-cadherin expression and tumor grade was also observed (p = 0.011). Decreased E-cadherin expression was more common in male patients with PDAC (81.3% vs. 60% for females, p = 0.005). γ-catenin and NEDD9 expressions were not statistically correlated with tumor stage and grade, gender, nor with patient survival. Our results support the role of NEDD9, E-cadherin and γ-catenin proteins in PDAC, but further research should clarify in detail their mechanism of action in pancreatic cancer.
Association of Basic Medical Sciences of FBIH
2018-08-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2378
10.17305/bjbms.2018.2378
Biomolecules and Biomedicine; Vol. 18 No. 3 (2018); 246-251
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2378/1105
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2378/1843
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2378/1844
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2499
2021-06-06T20:44:22Z
bjbms:PAT
driver
TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro
Ge, Yanli
Zhang, Junjie
Cao, Jianchun
Wu, Qiong
Sun, Longe
Guo, Likun
Wang, Zhirong
Trefoil Factor Family 1
gastric cancer
apoptosis
in vitro
Trefoil Factor Family (TFF) plays an essential role in the intestinal epithelial restitution, but the relationship between TFF1 and gastric cancer (GC) is still unclear. The present study aimed to determine the role of TFF1 in repairing gastric mucosa and in the pathogenesis of GC.
The TFF1 expression in different gastric mucosas was measured with immunohistochemistry. Then, siRNA targeting TFF1 or plasmids expressing TFF1 gene were transfected into BGC823 cells, SGC7901 cells and GES-1 cells. The cell proliferation was detected with MTT assay and apoptosis and cell cycle measured by flow cytometry.
From normal gastric mucosa to mucosa with dysplasia and to gastric cancer, the TFF1 expression had a decreasing trend. Down-regulation of TFF1 expression significantly reduced the apoptosis of three cell lines and markedly facilitated their proliferation but had no significant effect on cell cycle. Over-expression of TFF1 could promote apoptosis of three cell lines and inhibit proliferation but had no pronounced effect on cell cycle. TFF1 can inhibit proliferation and induce apoptosis of GC cells in vitro.
Association of Basic Medical Sciences of FBIH
2012-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2499
10.17305/bjbms.2012.2499
Biomolecules and Biomedicine; Vol. 12 No. 2 (2012); 74-81
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2499/444
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2499/2286
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2519
2021-01-05T19:32:56Z
bjbms:PAT
driver
Standardization and optimization of fluorescence in situ hybridization (FISH) for HER-2 assessment in breast cancer: A single center experience
Bogdanovska-Todorovska, Magdalena
Petrushevska, Gordana
Janevska, Vesna
Spasevska, Liljana
Kostadinova-Kunovska, Slavica
Breast cancer
HER-2
fluorescent in situ hybridization
FISH
standardization
IHC
immunohistochemistry
concordance
Accurate assessment of human epidermal growth factor receptor 2 (HER-2) is crucial in selecting patients for targeted therapy. Commonly used methods for HER-2 testing are immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Here we presented the implementation, optimization and standardization of two FISH protocols using breast cancer samples and assessed the impact of pre-analytical and analytical factors on HER-2 testing. Formalin fixed paraffin embedded (FFPE) tissue samples from 70 breast cancer patients were tested for HER-2 using PathVysion™ HER-2 DNA Probe Kit and two different paraffin pretreatment kits, Vysis/Abbott Paraffin Pretreatment Reagent Kit (40 samples) and DAKO Histology FISH Accessory Kit (30 samples). The concordance between FISH and IHC results was determined. Pre-analytical and analytical factors (i.e., fixation, baking, digestion, and post-hybridization washing) affected the efficiency and quality of hybridization. The overall hybridization success in our study was 98.6% (69/70); the failure rate was 1.4%. The DAKO pretreatment kit was more time-efficient and resulted in more uniform signals that were easier to interpret, compared to the Vysis/Abbott kit. The overall concordance between IHC and FISH was 84.06%, kappa coefficient 0.5976 (p < 0.0001). The greatest discordance (82%) between IHC and FISH was observed in IHC 2+ group. A standardized FISH protocol for HER-2 assessment, with high hybridization efficiency, is necessary due to variability in tissue processing and individual tissue characteristics. Differences in the pre-analytical and analytical steps can affect the hybridization quality and efficiency. The use of DAKO pretreatment kit is time-saving and cost-effective.
Association of Basic Medical Sciences of FBIH
2018-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2519
10.17305/bjbms.2018.2519
Biomolecules and Biomedicine; Vol. 18 No. 2 (2018); 132-140
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2519/827
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2519/1827
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2533
2021-06-06T20:26:46Z
bjbms:PAT
driver
C-Erb-b2 Oncogene Expression in Intraductal Proliferative Lesions of the Breast
Eren, Filiz
Calay, Zerrin
Durak, Haydar
Eren, Bülent
Çomunoğlu, Nil
Aydin, Övgü
breast
proliferative lesions
C-Erb-b2
The ductal intraepithelial neoplasia (DIN) classification which proposes new approaches to the diagnosis, terminology and differential diagnosis of intraductal proliferative lesions of the breast was applied to a series of female patients comprising C-erbB2 oncogene expression which may serve as an adjunct to the morphology by immunohistochemistry. The study was performed using the data of 94 patients. There was no difficulty encountered in the diagnosis of intraductal hyperplasia (IDH). In patients with Atypical Ductal Hyperplasia (AIDH), the diagnosis could be made by using the 2-mm rule of the DIN classification in patients who exhibited cytologic and structural characteristics of Ductal Carcinoma in Situ (DCIS) alone or in conjunction with classical IDH patterns. However, in lesions that mimicked classical IDH patterns despite displaying cytological features of in situ carcinomas, the experience and view point of the pathologist played a more prominent role. When the DIN classification criteria were applied to grade DCIS lesions, although the system was found to be practical, it did not provide adequate differentiation in intermediate grade (grade II-DIN 2) patients and further improvement was considered desirable. Fourty-five cases (47.8%) IDH, 19 (20.2%) AIDH, and 30 (31.9%) were DCIS. There were statistically significant differences in the levels of c-erbB2 oncogene expression between IDH, AIDH and DCIS lesions (p<0.001). In DCISs, grade, cell size, pleomorphic nuclear atypia showed statistically significant associations with c-erbB2 oncogene expression. These results suggest that c-erbB2 oncogene expression is a valuable marker in the differential diagnosis and prognostic evaluation of patients with intraductal proliferative lesions.
Association of Basic Medical Sciences of FBIH
2012-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2533
10.17305/bjbms.2012.2533
Biomolecules and Biomedicine; Vol. 12 No. 1 (2012); 41-50
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2533/471
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2533/2280
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2560
2021-06-06T20:02:02Z
bjbms:PAT
driver
Bronchioloalveolar differentiation in lung adenocarcinomas
Đukić, Nevena
Babić, Božana
Eri, Živka
Zec, Dragana
Lovrenski, Aleksandra
Kolarov, Violeta
lung
adenocarcinoma
bronchioloalveolar carcinoma
alcian blue
immunohistochemistry
The goals of the study were to determine what percentage of neoplasms with a bronchioloalveolar (BAC) component were considered pure BAC by current World Health Organization (WHO) criteria. Next, we wanted to determine the number of mucinous BACs using histochemical staining with Alcian Blue PAS. Finally, we aimed to elucidate by immunohistochemistry the thyroid transcription factor-1 (TTF-1) frequency and cytokeratin 7 (CK7) expression, particularly in regard to the mucinous and non-mucinous subtypes of BAC tumors. We made a retrospective review of Hematoxylin and Eosin stained slides and classification of histologic grade, tumor subtype, and percentage of pure BAC pattern, with further characterization by histochemical staining for Alcian Blue PAS and Immunohistochemical staining for thyroid TTF-1 and CK7. Only 10 of 30 tumors examined could be classified as BAC by current strict WHO criteria. Nine cases were classified into non-mucinous and only one case was recognized as mucinous BAC, which showed positive staining for Alcian Blue PAS. TTF-1 positivity was in 100% of the non-mucinous BACs and complete absence of staining was in one case of mucinous BACs. CK7 expression in bronchioloalveolar carcinoma has demonstrated CK7 marked staining in 90% of non-mucinous BACs, also one case of mucinous BACs showed marked staining for TTF-1. BACs of mucinous morphology were notable for their conspicuous absence of TTF-1 immunoreactivity.
Association of Basic Medical Sciences of FBIH
2011-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2560
10.17305/bjbms.2011.2560
Biomolecules and Biomedicine; Vol. 11 No. 4 (2011); 248-252
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2560/484
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2560/2270
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2594
2021-06-03T21:28:33Z
bjbms:PAT
driver
Skin and kidney histological changes in graft-versus-host disease (GVHD) after kidney transplantation
Pintar, Tadeja
Alessiani, Mario
Pleskovič, Alojz
Pleskovič, Aleš
Zorc-Pleskovič, Ruda
Milutinović, Aleksandra
experimental model
graft versus host disease
kidney transplantation skin biopsies
Kidney transplantation (Ktx) is generally performed during end stage renal disease due to a loss of the kidneys’ ability to filter wastes from the circulatory system. Acute graft-versus-host disease (GVHD) after Ktx is a life-threatening complication that progresses to organ failure, systemic complications, and death. The current study evaluated the significance of histologic findings of GVHD as obtained from skin biopsies following Ktx in swine. A swine model of Ktx with tacrolimus-based immunosuppression was used to assess possible correlations between acute-graft-cellular rejection and skin histological findings for prediction of GVHD. Animals were divided into a Ktx treatment group or a control group with no Ktx and skin and kidney biopsies were histologically assessed at postoperative days 0, 15, 30, 45 and 60. Skin samples were analyzed and classified from grade 1 to 4 of skin GVHD and the major histopathological changes of kidney acute cellular rejection were described using Banff’s score system. We observed a significant linear correlation between the histological grading values of skin biopsy changes and the histological grading values of kidney biopsies (Kendall’s tau_b=0.993) in the Ktx experimental group. No histological changes were observed in controls. Our findings demonstrate the diagnostic value of staging skin GVHD after Ktx and suggest it’s future utility for monitoring long term Ktx-induced changes.
Association of Basic Medical Sciences of FBIH
2011-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2594
10.17305/bjbms.2011.2594
Biomolecules and Biomedicine; Vol. 11 No. 2 (2011); 119-123
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2594/509
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2594/2242
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2660
2020-03-03T12:03:13Z
bjbms:PAT
driver
Correlation of cell cycle regulatory proteins (p53 and p16ink4a) and bcl-2 oncoprotein with mitotic index and thickness of primary cutaneous malignant melanoma
Kostov, Miloš
Mijović, Žaklina
Mihailović, Dragan
Cerović, Snežana
Stojanović, Miroslav
Jelić, Marija
melanoma
skin
cell cycle regulators
bcl-2
melanoma progression
The purpose of the study was to determine the frequency of expression p53 and p16INK4a proteins and bcl2- oncoprotein in malignant skin melanoma and to determine their correlation with the proliferative index and tumor thickness. The study involved 53 patients: 27 (51%) male and 26 (49%) female. Mitotic index showed a correlation with p53 protein expression, a negative correlation with p16INK4a protein expression. Statistically significant correlations were determined between the Breslow tumor thickness, Clark invasion level and p53 protein expression, as well as Breslow tumor thickness and bcl-2 oncoprotein expression (p<0.05), whereas there was no correlation between the p16INK4a protein expression and melanoma thicknes and Clark invasion level. Overexpression p53 protein and bcl-2 oncoprotein, with the loss p16INK4a protein of expression in the nodular melanoma, confirms a frequent loss of function of these tumor suppressor gene and oncogene, and indicates a vertical tumor growth phase. The loss of tumor suppression function the p53 protein and bcl-2 oncoprotein overexpression in cutaneous melanoma correlates with larger tumor thickness, whereas the overexpression of mutated p53 protein and loss p16INK4a protein of expression indicate a higher proliferative tumour potential. Therefore, these evaluated proteins may be the aggressive biological tumour activity markers.
Association of Basic Medical Sciences of FBIH
2010-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2660
10.17305/bjbms.2010.2660
Biomolecules and Biomedicine; Vol. 10 No. 4 (2010); 276-281
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2660/552
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2661
2020-03-03T12:05:50Z
bjbms:PAT
driver
The relationship between human leukocyte antigens (HLA) and renal cell carcinoma
Yılmaz, Erkan
Çekmen, Arman
Akkuş, Emre
Önal, Bülent
Uğur Özalp, Ali
Solok, Vural
Erdoğan, Ergun
Eren, Bülent
renal cell carcinoma (RCC)
human leukocyte antigens (HLA)
immunity
Etiologies of Renal Cell Carcinoma (RCC) are not clear despite of the fact that many risk factors have been suggested. Especially in high stages RCC can affect the immune system in various ways. Human Leukocyte Antigens (HLA) may play a complementary role in the activation between the tumor and immunity. Our aim was to determine the existence of the relationship between HLA system and RCC. By using the standard microlymphocytotoxic method of Terasaki in our study, the HLA A, B, DR and DQ antigen types of 20 patients with RCC Stage Ti and T2 were compared with the control group consisting of healthy 30 people. In our RCC patient group, HLA-A23(9) and DQ7(3) antigens were significantly higher than the control group statistically (p=0.005, p=0.0028; respectively). HLA-A10, DQi, DR10 and B44 antigens were significantly higher in the control group than the patient group (p=0.011; for all). The findings made us suggest that the people, carrying the antigens which were detected in the patient group, were at high risk for RCC and the people, carrying the protective antigens that were detected in the control group were at less risk for RCC. There may be a dramatic regression for the patients who underwent immunotherapy and HLA expression, which is known to play role in tumor biology, may direct the effects of immunotherapeutic agents. Immunologic description and destruction is avoided in case of change or disappearance of HLA expression by cancer cells. Further investigations which will be performed in our population in the future will be more illuminating to confirm those results. We have concluded that, HLA profiles may be evaluated for detection the people at risk of RCC, the prognosis of the patients and their treatments.
Association of Basic Medical Sciences of FBIH
2010-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2661
10.17305/bjbms.2010.2661
Biomolecules and Biomedicine; Vol. 10 No. 4 (2010); 282-286
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2661/553
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2677
2020-03-03T12:04:33Z
bjbms:PAT
driver
Analysis of pathohistological characteristics of pterygium
Džunić, Boban
Jovanović, Predrag
Veselinović, Dragan
Petrović, Aleksandar
Stefanović, Ivan
Kovačević, Igor
pterygium
recurrence
pathohistological characteristics
Pterygium internum (external eye layer) shows great recurrence tendency after surgical removal. Its etiology is still unclear and represents a significant problem. The main goal of our study was to explore the interrelationships of pathohistological characteristics of pterygium, namely presence of inflammation, vascularisation degree and fibrinoid changes and on the basis of their analysis to test the possibility of predicting its evolution and recurrence. The analysis was performed on the material taken from 55 patients surgically treated by the technique of Arlt. The specimens were stained using the classical histochemical methods: hematoxylin-eosin (HE), Masson’s trichrome, Gomori’s reticulin stain and PAS technique. Pterygium is mostly covered by conjunctival epithelium, while in the cap region shows morphology of modified stratified squamous epithelium of the cornea. Structural basis of the epithelium is composed of continuous basal lamina and continuous connective fibers underneath. This connective basis shows fibrinoid changes in the form of oval islets of different size, parallel to convexity of pterygium, or is in the form of unified focus. The number, caliber and the type of blood vessels showed excessive variability.
Pathohistological analysis of morphological characteristics of pterygium is adequate basis for prediction of recurrences; as they present the biggest concern in treatment of this widely spread disease.
Association of Basic Medical Sciences of FBIH
2010-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2677
10.17305/bjbms.2010.2677
Biomolecules and Biomedicine; Vol. 10 No. 4 (2010); 307-313
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2677/560
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2714
2020-03-03T12:41:00Z
bjbms:PAT
driver
Quantitative Research of Capillaries in Terminal Villi of Mature Placentae
Žigić, Zlata
Marković, Sergije
Grbeša, Đurđica
Ramić, Suada
Halilović, Almir
older pregnant women
placenta
terminal villi
capillaries
stereology
Advanced maternal age is known to be a risk factor for placental dysfunctions. The most common obstetric complications among older women would be considered as follows: gestational diabetes; preeclampsia; placenta praevia; preterm premature rupture of membranes and the risk of preterm delivery. The aims of research were to determine the impact of maternal age on the structure of terminal villi. The study was conducted on 60 human placentae of term pregnancy divided into two groups: the control group (30 placentae in pregnant women of age between 20 and 34) and the experimental group (30 placentae in pregnant women of 35 years of age and older). Stereological methods were applied to determine the volume density, surface density, total volume and total capillary surface area in terminal villi of placenta. The mean value of volume density of capillaries in terminal villi of placentae in older pregnant women is: Vvkks = (0,376 ± 0,033) mm°, and the mean value of total volume is: Vkks = (157,047 ± 25,022) cm3. The mean value of surface density is: Svkks = (64,783 ± 2,543) mm-1, and the mean value of total surface area is: Skks = (29,959 ± 7,873) m2. Volume density of capillaries in terminal villi of placentae is significantly lower in older pregnant women (p<0,001) in comparison to the younger pregnant women. The total volume, surface density and total capillary surface area in terminal villi of placentae are also significantly lower in older pregnant women (p<0,005) in comparison to the younger pregnant women. Statistically significant lower values of volume density, total volume, surface density and total capillary surface area indicate that there is a decreased metabolic transfer between mother and foetus.
Association of Basic Medical Sciences of FBIH
2010-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2714
10.17305/bjbms.2010.2714
Biomolecules and Biomedicine; Vol. 10 No. 2 (2010); 147-152
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2714/591
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2730
2020-03-04T11:40:50Z
bjbms:PAT
driver
Ultrastructural Changes in the Liver of Intravenous Heroin Addicts
Ilić, Goran
Karadžić, Radovan
Kostić-Banović, Lidija
Stojanović, Jovan
Antović, Aleksandra
intravenous heroin intake
ultrastructural liver lesions
The ultrastructural research has a decisive role in gathering the knowledge on the liver’s response to the influence of some drugs. The aim of the study was to perform an ultrastructurai analysis of the liver in chronic intravenous heroin addicts.
The study involved the autopsy conducted on 40 bodies of intravenous heroin addicts and 10 control autopsies. The liver tissue was fixed in glutaraldehyde and moulded with epon for investigation purposes of ultrastructural changes. The analysis was performed using the method of transmission electron microscopy.
In the group of intravenous heroin addicts, the liver autopsy samples showed degenerative vesicular and fat changes, chronic active and persistent hepatitis, cirrhosis, reduction in the amount of glycogen in hepatocytes, as well as the Kupffer cell’s dominant hypertrophy. Various changes occur in organelles, plasma membrane of hepatocytes and biliary channels as well as in the nucleus.
The most important ultrastructural findings include: hyperplasia and hypertrophy of the smooth endoplasmic reticulum, which is histologically proven vesicular degeneration of hepatocyte occurring as a result of the increased synthesis of enzymes of smooth endoplasmic reticulum due to chronic intravenous heroin intake, and the presence of continuous basal membrane followed by transformation of the sinusoids into capillaries (in the cases of chronic active hepatitis and cirrhosis) which leads to a disorder of microcirculation and further progress of cirrhosis.
Association of Basic Medical Sciences of FBIH
2010-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2730
10.17305/bjbms.2010.2730
Biomolecules and Biomedicine; Vol. 10 No. 1 (2010); 38-43
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2730/601
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2790
2020-03-05T08:17:38Z
bjbms:PAT
driver
The Study of Volume Density of Tracheal Ganglions In Vitro in New Born Babies with Respiratory Distress Syndrome
Shabani, Ragip
Islami, Hilmi
Bexheti, Sadi
Zeqiri, Fehmi
Dacaj, Ramadan
Kurtishi, Ilir
Haliti, Naim
Beqiri, Ruke
Shahini, Labinot
trachea
ganglion
stereometry
Volume density of respiratory organs was studied in vitro in newborn babies at different age of gestation (abort, immature, premature and mature) using stereometric method. The total of 23 cases was subject to this study. The respiratory organs (trachea, lungs) were taken from autopsies of newborn babies exited from different causes. For this purpose the tissues were fixed in formalin (10%) solution, cut serially in 7μ and 10μ slabs. Volume density of the respiratory system was assessed stereometricaly using Universal testing system Weibel M 42. We observed that volume density of epithelia, musculature and glands were proportionally present in the tracheal tissue. Cellular interstitial tissue is consistently increasing and corresponds to the developmental stages of the newborn babies.
The density of tracheal ganglions is greater in premature ages of immature and premature newborns (p<0,05). Decreased number of ganglion cells is observed in mature ages (p<0,05). This is caused by intensive ramification of ganglions from serosa to deeper layers of trachea right to epithelium. Medium diameter of tracheal ganglions is greater in mature newborn babies and corresponds to developmental ages of babies.
Association of Basic Medical Sciences of FBIH
2009-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2790
10.17305/bjbms.2009.2790
Biomolecules and Biomedicine; Vol. 9 No. 4 (2009); 335-341
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2790/639
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2831
2020-03-05T08:54:39Z
bjbms:PAT
driver
Immunohistochemical Expression of Tissue Inhibitor of Metalloproteinase-1 (Timp-1) in Invasive Breast Carcinoma
Kuskunović, Suada
Radović, Svjetlana
Dorić, Mirsad
Hukić, Ajna
Babić, Mirsad
Tomić, Ivana
Selak, Ivan
breast carcinoma
tissue inhibitor of metalloproteinase-1
immunohistochemistry
Tissue inhibitor of metalloproteinase-1 (TIMP-1) is a natural inhibitor of matrix metalloproteinas-es (MMPs). Aim of this study was to assess the immunohistochemical expression of TIMP-1 in invasive breast carcinomas, and to examine its association with classical clinico-pathological parameters, oestrogen receptor, progesterone receptor and Her-2/neu protein expression. Immuno-histochemistry was used to determine the expression of TIMP-1 on 38 paraffin-embedded breast tissue specimens - 18 with invasive ductal carcinoma, 10 with invasive lobular carcinoma, and 10 specimens from patients with fibrocystic breast disease. TIMP-1 protein was immunodetected in the carcinoma cells, fibroblasts and inflammatory cells of the stroma in 92,9%, 65,8%, and 65,8% of cases, respectively. TIMP-1 protein expression in carcinoma cells showed positive correlation with TIMP-1 protein expression in peritumoural fibroblasts (p=0,010). Positive peritumoural fibroblast TIMP-1 expression was associated with histological tumour type with higher frequency in ductal carcinomas (p=0,023). Negative association was found between TIMP-1 protein expression in carcinoma cells and HER-2/neu nuclear staining (p=0,005). TIMP-1 may be particularly useful as a predictive marker in breast carcinoma when evaluated along with HER-2/neu protein being a promising indicator of favourable prognosis in breast carcinoma.
Association of Basic Medical Sciences of FBIH
2009-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2831
10.17305/bjbms.2009.2831
Biomolecules and Biomedicine; Vol. 9 No. 2 (2009); 125-130
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2831/660
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2851
2020-03-09T12:49:26Z
bjbms:PAT
driver
Colorectal Cancer: Prognostic Values
Manxhuka-Kerliu, Suzana
Telaku, Skender
Ahmetaj, Halil
Baruti, Arijeta
Loxha, Sadushe
Kerliu, Agron
circumferential margin
colon cancer
grade
pathology
prognostic factors
Dukes’ system
After lung cancer colorectal cancer (Cc) is ranked the second, as a cause of cancer-related death. The purpose of this study was to analyze the Cc cases in our material with respect to all prognostic values including histological type and grade, vascular invasion, perineural invasion, and tumor border features. There were investigated 149 cases of resection specimen with colorectal cancer, which were fixed in buffered neutral formalin and embedded in paraffin. Tissue sections (4(µm thick) were cut and stained with H&E. Adenocarcinoma was the most frequent histological type found in 85,90% of cases, in 60,94% of males and 39,06% of females; squamous cell carcinoma in 7,38%, in 63,63% of males and 36,36% of females; mucinous carcinoma in 4,68%, in 57,15% of males and 42,85% of females; while adenosquamous carcinoma, undifferentiated carcinoma and carcinoma in situ in 0,71% of cases each. Dukes' classification was used in order to define the depth of invasion. Dukes B was found in 68,45% of cases, whereas in 31,54% of cases Dukes C was found. As far as histological grading is concerned, Cc was mostly with moderate differentiation (75,16%) with neither vascular nor perineural invasion. Resection margins were in all cases free of tumor. Our data indicate that the pathologic features of the resection specimen constitute the most powerful predictors of postoperative outcome in Cc. Dukes' stage and degree of differentiation provide independent prognostic information in Cc. However, differentiation should be assessed by the worst pattern.
Association of Basic Medical Sciences of FBIH
2009-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2851
10.17305/bjbms.2009.2851
Biomolecules and Biomedicine; Vol. 9 No. 1 (2009); 19-24
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2851/670
Copyright (c) 2017 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2921
2020-03-10T10:54:35Z
bjbms:PAT
driver
In Vitro Examination of Degenerative Evolution of Adrenergic Nerve Endings in Pulmonary Inflamatory Processes in Newborns
Islami, Hilmi
Shabani, Ragip
Haliti, Naim
Bexheti, Sadi
Koliqi, Rozafa
Raka, Denis
Sukalo, Aziz
Izairi, Rusmi
Dauti, Hilmi
Qehaja, Nazim
adrenergic receptors
human trachea
bronchus and pulmonary tissue
Morphological aspect of tracheal preparations and pulmonary tissue was studied in vitro. The material was obtained from autopsy of newborns that died from different causes. Examinations were made in different gestational periods (immature 23-29 weeks; premature 30-37 weeks; mature >38 weeks). Material for examination was obtained up to 6 hours after death. Pulmonary and tracheal tissue was incubated for fixation in buffered formalin (10%). Special histochemical and histoenzymatic methods were used for coloring of pulmonary and tracheal tissue and the activity of ATP-ase and dopaoxidase was monitored. Cut out models were made in series of 7μ, 10 μ and 20 μ. In peripheral axons of tracheobronchial pathways, degenerative alterations of adrenergic nerve endings in lung inflammatory processes were documented. These morphologic neuronal changes were described: Walerians degeneration, neuro-axonal degeneration and segment demyelinisation. These changes are well seen with argentafine coloring (Sevier-Munger modification for nerve endings) and with dopaoxidase reaction. In mature newborns that died from respiratory distress syndrome, we found different forms of metabolic and toxic degenerative damage in peripheral axons, such as: segment demyelinisation, neurotubular fragmentation, Schwan cell proliferation, fragmentation and bulging out of axonal neurotubules and neurofilaments. In tracheo-bronchial tissue, chromafine granules are homogenously distributed on Lamina propria layer and through glandular structures. This gives as a contradiction, according to some authors, that adrenergic nerve fibers for muscle tissue are absent and that adrenaline and noradrenalin diffuse in muscle tissue from interstice.
Association of Basic Medical Sciences of FBIH
2008-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2921
10.17305/bjbms.2008.2921
Biomolecules and Biomedicine; Vol. 8 No. 3 (2008); 220-225
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2921/705
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2951
2021-01-09T14:00:01Z
bjbms:PAT
driver
Immune cells and vasa vasorum in the tunica media of atherosclerotic coronary arteries
Zorc-Pleskovič, Ruda
Pleskovič, Aleš
Vraspir-Porenta, Olga
Zorc, Metka
Milutinović, Aleksandra
Atherosclerosis
coronary arteries
tunica media
T cells
B cells
macrophages
immune privilege
vasa vasorum
In coronary artery disease (CAD), the disruption of the tunica media immune privilege manifests as increased leukocyte infiltration and the formation of vasa vasorum. We aimed to characterize the immune privilege status of the tunica media in human coronary arteries (CAs) with atherosclerotic plaques, by comparing the abundance and composition of immune-cell infiltrates within the individual arterial-wall layers, and by evaluating vasa vasorum neovascularization of the tunica media. The tissue samples were obtained from 36 symptomatic patients with diffuse CAD (aged 60–72 years) who underwent coronary endarterectomy. T and B cells, macrophages and endothelial cells in the CAs were detected by immunohistochemistry. Morphological analysis of CAs showed significant atherosclerotic changes in all specimens. In the media, we observed damage and loss of smooth muscle cells, destruction of the extracellular matrix architecture, and fibrosis. There were 43.3% of immune cells in the intima, 50% in the adventitia, and 6.7% in the media. In the media, 51.1% of the immune cells were T cells (p ˂ 0.001 compared to B cells and macrophages; ANOVA, Scheffe post hoc analysis), 23.5% were B cells, and 25.4% were macrophages. The number of vasa vasorum in the media was 1 in 38.9% of CAs, 2–3 in 36.1%, and ≥4 in 25% of CAs. Our results indicate that, in atherosclerotic CAs, the immune privilege of the media is disrupted by the infiltration of T and B cells, macrophages, and the presence of vasa vasorum.
Association of Basic Medical Sciences of FBIH
2018-08-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2951
10.17305/bjbms.2018.2951
Biomolecules and Biomedicine; Vol. 18 No. 3 (2018); 240-245
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2951/1048
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2951/1842
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2965
2020-03-10T11:27:23Z
bjbms:PAT
driver
Urinary System Birth Defects in Surgically Treated Infants in Sarajevo Region of Bosnia and Herzegovina
Aličelebić, Selma
Kapić, Dina
Mornjaković, Zakira
congenital anomalies
urinary system
frequency
sex distribution
Congenital anomalies of the urinary system are relatively common anomalies. In Bosnia and Herzegovina there is no existent unique evidence of congenital anomalies and registries. The aim of this study was to obtain the frequency of different urinary tract anomalies types and their sex distribution among cases hospitalized in the Department of Pediatric Surgery of the University of Sarajevo Clinics Centre, Bosnia and Herzegovina, during the period from January 2002 to December 2006. Retrospective study was carried out on the basis of clinical records. Standard methods of descriptive statistics were performed for the data analysis. Among 289 patients that were surgically treated 62,37% of the patients were male patients, while 37,63% were female patients. Twenty nine different urinary system anomalies types were found in this study. These were: vesicoureteral reflux (99 cases or 30,75%), hypospadias (62 cases or 19,26%), pelviureteric junction obstruction (42 cases or 13,04%), megaureter (35 cases or 10,87%), duplex pelvis and ureter (16 cases or 4,97%), bladder diverticulum (8 cases or 2,48%), ureterocoele (7 cases or 2,17%), stenosis of the external urethral opening (6 cases or 1,86%), ectopic kidney, duplex kidney and pelvis (each 5 cases or 1,55%), polycystic kidneys and urethral stricture (each 4 cases or 1,24%), multicystic kidney (3 cases or 0,93%), kidney agenesis, ureter agenesis, urethral diverticulum, ectopic ureter, horseshoe kidney and fetal kidney (each 2 cases or 0,62%), renal aplasia, urethral atresia, renal cyst, urachal cyst, epispadias, bladder exstrophy, renal hypoplasia, renal malrotation and Prune-Belly syndrome (each 1 case or 0,31%). According to this study, urinary tract anomalies were more common in male than in female patients (62,37%), Generally, the most frequent anomaly type was vesicoureteral reflux in total number of 99 cases, and in females (66 cases), but hypospadias was the most common anomaly in males (62 cases). The anomalies of other systems associated with urinary system anomalies were found in ten cases. These were: cryptorchidism, congenital inguinal hernia, open inner inguinal ring, uterus bicornis unicollis and one case of multiple anomalies.
Association of Basic Medical Sciences of FBIH
2008-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2965
10.17305/bjbms.2008.2965
Biomolecules and Biomedicine; Vol. 8 No. 2 (2008); 126-130
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2965/725
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/2972
2020-03-10T11:28:27Z
bjbms:PAT
driver
Dermal Squamomelanocytic Tumor: Neoplasm of Uncertain Biological Potential
Dorić, Mirsad
Radović, Svjetlana
Kuskunović, Suada
Hukić, Ajna
Babić, Mirsad
Tomić, Ivana
Selak, Ivan
squamomelanocytic
squamous cell carcinoma
malignant melanoma
We report a case of exceedingly rare cutaneous neoplasm with histological features of malignancy and uncertain biological potential. The nodular, darkly pigmented facial tumor with central exulceration, size 12x10x7 mm, of the skin 61-year-old man preauricular left was completely exised.
Histologically tumor consists of atypical squamous cells, which express signs of moderate to significant pleomorphism, mitotically active, with foci forming of parakeratotic horn cysts (“pearls”). Characteristically tumor also consists of large number of atypical melanocytes with multifocal pattern, inserted between atypical squamous cells, and which contain large amount of dark brown pigment melanin. Immunohistochemically, squamous cells stain positively with keratin (CK116), melanocytes were stained with S -100 protein, HMB 45, and vimentin, but failed to stain with CK 116.
To our knowledge this is the sixth reported case in world literature. The follow-up time of four years no evidence of recurrence or metastasis, similar all reported cases, but it is too short period in estimation to guarantee a benign course. However, it appears that this group of neoplasm may have different prognosis from pure squamous carcinoma or malignant melanoma.
Association of Basic Medical Sciences of FBIH
2008-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2972
10.17305/bjbms.2008.2972
Biomolecules and Biomedicine; Vol. 8 No. 2 (2008); 152-155
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/2972/730
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3045
2020-07-23T12:52:16Z
bjbms:PAT
driver
Non-Small Cell Lung Carcinoma: Cyclin D1, bcl-2, p53, Ki-67 and Her-2 Proteins Expression in Resected Tumors
Radović, Svjetlana
Babić, Mirsad
Dorić, Mirsad
Hukić, Ajna
Kuskunović, Suada
Hadžismajlović, Ademir
Serdarević, Fadila
cyclin D1
bcl-2
p53
Ki-67
HER-2
immunohistochemistry
non-small cell lung cancer
The aim of this study was to investigate expression of cyclin D1, bcl-2, p53, Ki-67 and HER-2 proteins in 14 cases of non-small cell lung cancer and to establish their correlation to classical clinico-pathological findings, and alleged prognostic value to estimate biological potential of tumor. Retrospective pilot study of the surgically treated non-small cell lung cancer biopsy specimen, paraffin embedded, used immu-nohistochemical method to demonstrate expression of cyclin D1, bcl-2, p53, Ki-67 and HER-2. Protein quantification was performed by the semi-quantitative method. Achieved results were correlated with classical clinico-pathological parameters, like tumor size, histological type, differentiation level, presence ofvascular invasion and metastasis in regional lymph nodes. Out of 14 cases of non-small cell lung cancer, squamous cell carcinoma was found in 7 patients, giant cell carcinoma in 3, adenocarcinoma in 2, and 1 case of pleomorphic and mucoepidermoid carcinoma. Expression of cyclin D1 was not found, while expression of HER-2 and bcl-2 protein was established in one cases each. p53 expression was noted in 8 cases (57,1%). Statistically positive significant correlation (p<0,05) was found among: presence of lympho-vascular invasion to tumor tissue and appearance of nodal metastasis; proliferation Ki-67 index and level of tumor differentiation, i.e. size of tumor. Other investigated parameters showed no significant statistically dependence. p53 expression was not correlated to any of the investigated parameters what might imply the possibility that there is an independent pathway of this protein expression. Negative expression of bcl-2 protein points out to possibility that it is not included into process of tumor apoptosis, as well as that proteins cyclin D1 and HER-2 are not included into processes of the tumor genesis. Since the prolif-erative activity of the tumor, measured by the expression of Ki-67, is correlated to the gradus and size of the tumor mass, Ki-67 protein can be of a prognostic value to determine biological potential of non-small cell lung cancer.
Association of Basic Medical Sciences of FBIH
2007-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3045
10.17305/bjbms.2007.3045
Biomolecules and Biomedicine; Vol. 7 No. 3 (2007); 205-211
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3045/775
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3080
2020-07-24T09:59:27Z
bjbms:PAT
driver
Tumor Suppresser Gene p53 Expression in Premalignant Lesions and Gastric Carcinoma - Prognostic Value
Vukobrat-Bijedić, Zora
Radović, Svjetlana
Husić-Selimović, Azra
Gornjaković, Srđan
intestinal metaplasia (IM)
epithelial dysplasia
Helicobacter pylori (H. pylori)
p53
The aim of the study was to verify the presence of mutated tumor suppresser gene p53 in intestinal mucosa with histologically confirmed premalignant lesions and gastric carcinoma, and assess its prognostic value. The paper presents prospective study that included 50 patients with gastric adeno-carcinoma of intestinal type that were treated at Gastroenterohepatology Clinic, and 50 patients with histologically confirmed chronic atrophic H. pylori positive gastritis. In the mucosa biopsy samples, we analyzed presence, frequency and severity of inflammatory-regenerative, metaplastic and dysplastic changes. We typed intestinal metaplasia immunohistochemically and confirmed the presence of p53 onco-protein in antigen positive gastric carcinoma cells, and evaluated its prognostic value. Our results suggest that H. pylori acts as an initiator of inflammatory processes in gastric mucosa, which are followed by emergence of precancerous lesions. p53 is expressed late in carcinogenesis (14%) and as such, may be considered as an indicator of transformation of premalignant into malignant lesion.
Association of Basic Medical Sciences of FBIH
2007-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3080
10.17305/bjbms.2007.3080
Biomolecules and Biomedicine; Vol. 7 No. 1 (2007); 7-10
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3080/809
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3092
2020-07-24T09:02:32Z
bjbms:PAT
driver
Association of Hodgkin's lymphoma with Epstein Barr virus infection
Čičkušić, Elmir
Mustedanagić-Mujanović, Jasminka
Iljazović, Ermina
Karasalihović, Zinaida
Škaljić, Ina
Hodgkin’s lymphoma
immunohistochemistry
Epstein-Barr virus
LMP-1
EBER RNK
in situ hybridization
The role of Epstein Barr virus (EBV) in the onset of Hodgkin's lymphoma has been a subject of ongoing research. However, confirmation of EBV oncogenic involvement was not possible due to the small number of neoplastic cells characteristic for this type of tumor. Presence of EBV infection in neoplastic and non-neoplastic cells was analyzed in 81 cases of Hodgkin's lymphoma. In neoplastic cells, using an immunohistochemical method, latent membrane protein 1 (LMP1) was found in 33,3% of cases, while in situ hybridization results demonstrated the presence of EBER RNA in 48,1% of the cases. EBER RNA was found in non-neoplastic lymphocytes in 38,3% of cases. EBV is most frequently associated with Hodgkin's lymphoma in the first and seventh decade of life, specifically the nodular sclerosis subtype. No apparent difference was observed in the association of Hodgkin's lymphoma with EBV between genders, or in relation to clinical stage of the disease and average age of the patient. However, association with childhood age is significantly greater in comparison to adults. EBV associated disease shows a significantly greater prevalence in T lymphocytes. Slightly more abundant are cytotoxic T lymphocytes, which are also more frequently in contact with Reed-Sternberg cells, although there is no difference in number and positioning of histiocytes. Variations between the data on the association of EBV with Hodgkin's lymphoma among studies from different parts of the world suggest that factors of age, gender, ethnic background and social status might present biological modifiers of EBV influence on the pathogenesis of this neoplasm. The differences in non-neoplastic infiltrate EBV+ and EBV- lymphoma indicate the effect of the virus on the immune interaction of tumor and host in this disease.
Association of Basic Medical Sciences of FBIH
2007-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3092
10.17305/bjbms.2007.3092
Biomolecules and Biomedicine; Vol. 7 No. 1 (2007); 58-65
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3092/820
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3093
2020-07-24T09:03:38Z
bjbms:PAT
driver
Zearalenone-induced Lymphophagocytosis (T Cell Apoptosis) on the Rat’s Thymus
Dorić, Mirsad
Radović, Svjetlana
Babić, Mirsad
Kuskunović, Suada
Tomić, Ivana
Selak, Ivan
apoptosis
lymphophagocytosis
thymus
zearalenone
The effects of nonsteroidal mycotoxin zearalenone on the lymphoid tissue of thymus in a sense of investigating the subacute toxicity Wistar-albino rats have been examined in the course of the study. We analyzed 42 rats' specimens of both gender, treated with three dosage levels: 0,5; 2 and 4 mg/kg of body weight, after oral submission of the compound, and observed during three different time intervals: 10, 20 and 30 days. Microscopically was semiquantitatively determined lymphophagocytosis (apoptosis) and cortical thymic cellularity. It was percepted statistically significant growth of lymphophagocytosis compared to a dosage (p<0,01), as well as combination of dosage and interval (p<0,001), while gender had no statistically significant influence on tested parameter (p>0,05). Changes in cortical thyme cellularity were not percepted. Effects of applied doses of zearalenone on the lymphoid tissue of thymus were very mild and in correlation with estrogenicity. They are probably the result of interaction with estrogenic receptors.
Association of Basic Medical Sciences of FBIH
2007-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3093
10.17305/bjbms.2007.3093
Biomolecules and Biomedicine; Vol. 7 No. 1 (2007); 66-70
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3093/821
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3094
2020-12-28T13:46:07Z
bjbms:PAT
driver
Prognostic value of aldehyde dehydrogenase 1 (ALDH1) in invasive breast carcinomas
Demir, Hale
Dulgar, Ozgecan
Gulle, Bugra Taygun
Turna, Hande
Ilvan, Sennur
ALDH1
breast carcinoma
invasive breast carcinoma
cancer stem cells
molecular subtype
Aldehyde dehydrogenase 1 (ALDH1) has been identified as a marker of cancer stem cells in breast cancer (BC). Recent studies showed that ALDH1 expression is correlated with poor prognostic parameters and worse clinical outcome in BC. We evaluated ALDH1 expression by immunohistochemistry in a series of 217 invasive BCs and investigated the correlation between ALDH1 expression and clinicopathological parameters, molecular subtypes (luminal A, luminal B, human epidermal growth factor receptor 2 [HER2] type, and triple-negative BC [TNBC]), and patient survival. There was a significant association between ALDH1 expression and tumor grade (p < 0.001), i.e., the expression of ALDH1 was higher in high-grade tumors. ALDH1 expression was significantly associated with estrogen and progesterone receptor (ER and PR) negativity (p < 0.001) and HER2 positivity (p = 0.001). ALDH1 expression ratios were higher in HER2 type and TNBC. There was a statistically significant correlation between ALDH1 negativity and luminal A subtype (p < 0.001). The overall and disease free survival were shorter in ALDH1+ tumors, although without statistical significance. We confirm that ALDH1 is a potentially important, poor prognostic factor in BC, associated with high histological grade, ER/PR negativity and HER2 positivity. For more accurate results, ALDH1 expression should be evaluated in larger case series including various types/subtypes of BC.
Association of Basic Medical Sciences of FBIH
2018-11-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3094
10.17305/bjbms.2018.3094
Biomolecules and Biomedicine; Vol. 18 No. 4 (2018); 313-319
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3094/1102
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3094/1789
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3112
2020-07-24T09:18:10Z
bjbms:PAT
driver
The Role of Pineal Gland and Exogenous Melatonin on the Irradiation Stress Response of Suprarenal Gland
Aličelebić, Selma
Mornjaković, Zakira
Šuško, Irfan
Ćosović, Esad
Beganović-Petrović, Amira
melatonin
suprarenal gland
pinealectomy
total irradiation
Pineal gland has and antistressogenic role. Its main hormone, melatonin, has radio protective effect on endocrine and other dynamic tissues. In our previous study, we have shown that pinealectomy changes the behavior of suprarenal gland in totally irradiated rats. The aim of this study is to evaluate the effect of exogenous melatonin on suprarenal gland of rats with or without pineal gland. Four months after pinealectomy (experimental group) or shampinealectomy (control group), adult Wistar male rats were daily treated with 0,2 mg of melatoninintraperitoneally, during two weeks. Thereafter, all animals were totally irradiated with 8 Gy of Gamma rays produced from Cobalt 60. Animals who survived were sacrificed on the 17(th) post irradiation day. Qualitative and quantitative characteristics of the suprarenal gland were studied using histological methods. The results show that exogenous melatonin had protective role on suprarenal gland in totally irradiated rats and that those effects were more pronounced in the presence of pineal gland.
Association of Basic Medical Sciences of FBIH
2006-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3112
10.17305/bjbms.2006.3112
Biomolecules and Biomedicine; Vol. 6 No. 4 (2006); 18-21
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3112/830
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3120
2020-07-24T09:19:26Z
bjbms:PAT
driver
Incomplete Intestinal Metaplasia as an Indicator for Early Detection of Gastric Carcinoma in The Events of Helicobacter Pylori Positive Chronic Atrophic Gastritis
Vukobrat-Bijedić, Zora
Radović, Svjetlana
Husić-Selimović, Azra
Gornjaković, Srđan
intestinal metaplasia (IM)
epithelial dysplasia
Helicobacter pylori (H. pylori)
chronic atrophic gastritis (CAG)
The aim of the study was to ascertain the existence of intestinal metaplasia in gastric mucosa of patients with gastric carcinoma coupled with H. pylori positive chronic atrophic gastritis and possible connection of IM with the development of gastric carcinoma. The paper presents prospective study that included 50 patients with gastric carcinoma and 50 patients with chronic atrophic H. pylori positive gastritis. All the patients were subjected to gastroscopy as well as biopsy targeted at antrum, lesser curvature and corpus and at the area 1-2 cm removed from tumor lesion. Biopsy samples were sliced by microtome and stained. We analyzed presence, frequency and severity of inflammatory-regenerative, metaplastic and dysplastic changes in the mucosa and evaluated their prognostic value. We typed IM immunohistochemically. This study confirmed responsibility of H. pylori for inflammatory events in gastric mucosa in patients with gastriccarcinoma. According to our findings incomplete IM of types IIa and IIb as precancerous lesion is responsible for the development of gastriccarcinoma and is associated with chronic atrophic gastritis grade I and II (92% of subjects, p=0.0097, h=1, p=0.01). Thus, the finding of incomplete intestinal metaplasia may be used as an indicator for early gastric carcinoma detection. Patients with patho-histologically verified incomplete intestinal metaplasia associated with active chronic atrophic gastritis of levels I and II represent risk group for the development of gastric carcinoma of intestinal type.
Association of Basic Medical Sciences of FBIH
2006-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3120
10.17305/bjbms.2006.3120
Biomolecules and Biomedicine; Vol. 6 No. 4 (2006); 48-53
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3120/838
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3122
2020-07-24T09:20:03Z
bjbms:PAT
driver
Cyclooxygenase-2 Expression Determines Neoangiogenesis in Gallbladder Carcinomas
Legan, Mateja
Luzar, Boštjan
Ferlan-Marolt, Vera
Cör, Andrej
gallbladder carcinoma
cyclooxygenase
tumor angiogenesis
endoglin
intratumoral microvessel density
Neo-angiogenesis may have an important role in the poor prognosis of gallbladder carcinoma. An enhanced expression of COX-2 was found in precancerous lesions and in gallbladder carcinoma, likely to be involved in carcinogenesis as well as in angiogenesis. To study the relationships between the COX-2 expression and degree of vascularization, as well as to evaluate their role in the prognosis of patients with gallbladder carcinoma. 27 cases of gallbladder adenocarcinoma were included, classified grading I-III according the WHO classification. The COX-2 and endothelial antigen CD105 expressions were assessed immunohistochemically. COX-2 expression was evaluated according to the percentage and staining intensity of positive cells into "COX-2 positive" and "COX-2 negative" groups. In order to assess tumor microvessel density (MVD), CD105 positively stained microvessels were counted for each specimen in predominantly vascular areas (hot spots) at 200 x magnification. The MVD ranged from 9 to 46 microvessels/field. 15 tumors belonged to the hypervascular group (MVD > or = 25) and 12 to the hypovascular group. There were 16 (59.2%) COX-2 positive cases. There was difference in the degree of angiogenesis between COX-2 positive vs. COX-2 negative group: 11 (68.8%) out of 16 "COX-2 positive" tumors were hypervascular, in comparison with just 4 (36.4%) of "COX-2 negative" tumors. Our data show that the MVD corresponds to the COX-2 overexpression in gallbladder carcinomas. Augmented tumor neovascularization induced by COX-2 might be responsible for the poor prognosis in gallbladder carcinoma patients.
Association of Basic Medical Sciences of FBIH
2006-10-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3122
10.17305/bjbms.2006.3122
Biomolecules and Biomedicine; Vol. 6 No. 4 (2006); 58-63
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3122/840
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3128
2020-07-24T09:21:17Z
bjbms:PAT
driver
Efficacy in Treatment of Cervical HrHPV Infection by Combination of Beta Interferon, and Herbal Therapy in Woman with Different Cervical Lesions
Iljazović, Ermina
Ljuca, Dženita
Šahimpašić, Ademir
Avdić, Silvija
human papilloma virus
cervical lesion
herbal therapy
Cervical dysplasia, a premalignant lesion that can progress to cervical cancer, is caused primarily by a sexually transmitted infection with an oncogenic strain of the human papillomavirus (HPV). The HPV infections are treated through destroying the clinical lesions: laser, cryotherapy, podophyllin. The hope is that by causing local tissue inflammation that the body will be stimulated to mount an antibody response and thereby prevent recurrence. In contrast to other prevention approaches, vaccines can reduce susceptibility in uninfected partners by stimulating the immune system. Aloe vera has also been reported to retard tumour growth and stimulate the immune response to viruses. A list of possible actions of propolis includes: antibacterial, antifungal, antiviral, antioxidant, anticarcinogenic, antithrombotic and immunomodulatory. Research on the possible role of some B vitamins in preventing cancer began in the last few decades, but however this complex have an influence on immune status. The aim of our study is to try to treat the HPV infection as confirmed cause of neoplastic transformation with some herbal therapy and interferon and to try define the guidelines in the management of the HPV positive patients. Goal of this paper is to search for evidence of efficacy of any treatment for HPV infection of the cervix mostly in woman with no concomitant CIN. Fifty five woman affected by HPV genital infection were enrolled in the study from September 2005 to April 2006. Patients were classified according to the results of the HPV testing prior and after the therapy. Patients were randomized into two groups: the first group was HPV positive woman treated with other than recommended therapy (n=20), (control group); the second group was pharmacologically treated with intravaginal administration of an interferon and aloe vera-propolis in recommended scheme (n=35) with treatment of the possible fungal or bacterial genital infection prior to the specific therapy. The almost same therapy was recommended to the male partner. Patients from the second group used B complex during the therapy. Patients were retested for the HPV presence after three or six month from therapy depend of the presence bacterial or fungal genital coinfection. Three months after applied therapy HPV infection was still present in more than 90% of the patients in the first group. In the second group treated according to the recommended therapy scheme HPV infection disappeared in 71.42% of the patients after three months and in 100% of patients after six months. Samples of the cervical smear for the HPV analysis were being taken during routine gynecological examinations, by using sticks with cotton, taken from the Digene Specimen Collection Kit, from the whole surface of a portion, and by mild rotating moves from the outer cervical entrance. Our results suggest that the combination of interferon and herbal therapy with B complex is effective, atraumatic and simple non-surgical treatment of HPV infection. Since prospective efficacy trials will take several years to complete, considering alternative approaches is also worthwhile.
Association of Basic Medical Sciences of FBIH
2008-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3128
10.17305/bjbms.2006.3128
Biomolecules and Biomedicine; Vol. 6 No. 4 (2006); 79-84
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3128/845
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3146
2020-07-24T09:34:12Z
bjbms:PAT
driver
Helicobacter Pylori as a Promoter of Accelerated Regeneration, Pathological Differentiation and Transformation of Normal Gastric Mucosa into Cancerous Type
Vukobrat-Bijedić, Zora
Radović, Svjetlana
Husić-Selimović, Azra
Gornjaković, Srđan
Helicobacter pylori (H. pylori)
intestinal metaplasia (IM)
epithelial dysplasia
chronic atrophic gastritis
The aim of the study was to ascertain presence of Helicobacter pylori in gastric carcinoma as a responsible promoter of inflammatory-regenerative changes, which lead to pathological differentiation and transformation of normal epithelial cells into intestinal type and, in progression, cause epithelial dysplasia that develops into early gastric carcinoma. The paper presents prospective study that includes clinical, pathohistological and microbiological aspects of carcinogenesis initiation in gastric mucosa. The subjects are patients treated at Gastroenterohepatology Clinic divided into two groups. One group included 50 patients with gastric carcinoma while the control group included 50 patients with chronic atrophic H. pylori positive gastritis. All the patients were subjected to endoscopy as well as biopsy targeted at antrum, lesser curvature and corpus and at the region 1-2 cm removed from tumor lesion. We used HUT test to verify H. pylori presence in biopsy samples. We analyzed the samples for presence, frequency and severity of inflammatory-regenerative, metaplastic and dysplastic changes in gastric mucosa and evaluated their meaning for the prognosis. Our study confirmed Helicobaster pylori responsibility for inflammatory events in gastric mucosa in patients with gastric carcinoma. Slight and mild epithelial dysplasia with chronic atrophic gastritis grade I and II coupled with intestinal metaplasia may be considered an indicator for early detection of carcinoma. Such patients represent risk group for gastric carcinoma development.
Association of Basic Medical Sciences of FBIH
2006-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3146
10.17305/bjbms.2006.3146
Biomolecules and Biomedicine; Vol. 6 No. 3 (2006); 57-60
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3146/856
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3161
2020-07-27T08:37:42Z
bjbms:PAT
driver
Stereological Analysis of Mature Human Placenta of Pregnant Women of Different Age
Ramić, Suada
Žigić, Zlata
Alečković, Mirna
placenta
pregnant women of different age
stereology
The normal placentas, regular pregnancies and deliveries were structurally examined. The aim of this research was to compare the results and to confirm if there were some difference in the structure of placenta related to the age of pregnant women. We examined 30 human placentas. The examined group of women were divided into two groups: 1) pregnant women 20-35 years old; 2) pregnant women over 35 years old. The stereological method was used. The volume density, absolute volume, the surface density and absolute surface of terminal villi of placentas in younger and older pregnant women were not significantly different. The volume density, absolute volume, the surface density and absolute surface of the other placentas villi in younger pregnant women compared to older ones, were significantly increased (p<0.001). The volume density of fibrinoid of placentas in older pregnant women compared to younger ones was significantly increased (p<0.02). The surface density, absolute volume and absolute surface of fibrinoid in these two examined groups of pregnant women were not significantly different. The volume density of intervillous space of placentas in older pregnant women compared to younger ones was significantly increased (p<0.05). Absolute volumes of intervillous space of placentas in these two examined groups of pregnant women are not significantly different.
Association of Basic Medical Sciences of FBIH
2006-05-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3161
10.17305/bjbms.2006.3161
Biomolecules and Biomedicine; Vol. 6 No. 2 (2006); 7-10
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3161/864
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3208
2020-07-27T08:57:27Z
bjbms:PAT
driver
Expression of p53, bcl-2, and ki-67 Proteins in the Inflammatory Regenerative and Dysplastic Epithelial Lesions of Flat Colonic Mucosa
Radović, Svjetlana
Vukobrat-Bijedić, Zora
Selak, Ivan
Babić, Mirsad
p63
bcl-2
Ki-67
epithelia dysplasia
flat mucosa of the colon
The aim of the study was to define the distribution of p53, bcl-2 and Ki-67 proteins in the inflammatory-regenerative and dysplastic lesionsof the colon mucosa. The relationship between the presentation of p53, bcl-2 and Ki-67 proteins and the intensity of the inflammatory-regenerative and dysplastic lesions in the colon flat mucosa was investigated as well. Biopsy specimens from 270 patients were examined: 74 were classified as inflammatory-regenerative and 196 as dysplastic lesions (108 mild, 58 moderate, and 30 severe dysplasia). The expression of all three proteins was assessed on the basis of location, quantity, and intensity of immunostaining, by counting antigen positive cells, in comparison with normal mucosa and adenocarcinoma. p53 protein appears only in sporadic cases (6.6%) of severe dysplasia. Bcl-2 expression appears significantly (p<0.005) more often in cases of mild dysplasia (61.1%) compared to inflammatory-regenerative mucosa (14.8%). In cases of mild dysplasia, bcl-2 positive cells were spreading from the lower third to the middle third of the crypts. Bcl-2 expression was maintained through the stadiums of moderate and severe dysplasia (75.8%), where antigen positive cells were found all along the crypts. A significant increase (p<0.005) in the expression of nuclear protein Ki-67 was noticed in the stadiums of moderate (labelling index =26.3) compared to mild dysplasia (labelling index=16.7), and severe (labelling index=36.7) compared to moderate dysplasia, where the zone of cellular proliferation was widen along the whole crypt length. In the process of the development of epithelial dysplasia in the flat mucosa of colon a degree of the gene p53 alteration is low and appears only in sporadic cases of severe dysplasia. Mutation of the bcl-2 gene is involved in the genesis of the lesion but not in its progression to carcinoma. Increased expressionof Ki-67 protein speaks in favour of an increased cellular proliferation which, together with the above mentioned mechanisms, is involved in the process of occurrence and progression of epithelial dysplasia in the flat mucosa of colon.
Association of Basic Medical Sciences of FBIH
2008-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3208
10.17305/bjbms.2006.3208
Biomolecules and Biomedicine; Vol. 6 No. 1 (2006); 39-45
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3208/887
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3327
2020-07-28T11:27:48Z
bjbms:PAT
driver
Correlation of the HER-2 protein expression and other clinicopathological features of ductal infiltrative breast cancer
Radović, Svjetlana
Babić, Mirsad
Dorić, Mirsad
Balta, Edina
Kapetanović, Erol
Šečić, Senad
HER-2
immunohistochemistry breast cancer
The aim of the study was to investigate the relationship between the expression of the HER-2 membrane protein and other clinical-pathological parameters such as: histological size of the tumor, degree of the tumor's differentiation, presence of vascular invasion and presence of metastases in regional lymph nodes, in cases of ductal infiltrative breast cancer. We have investigated 56 cases of ductalinfiltrative breast cancer. In all patients a mastectomy with a dissection of axillary lymph nodes has been performed. All tissue samples, taken by biopsy, were embedded in the paraffin, stained by hematoxylin-eosin technique and screened, and evaluation was performed by using a semiquantitative method of the immunohistochemical expression of the HER-2 protein. A decrease of the protein HER-2 expression was noticed in cases of an increase of the tumor's diameter above 50 mm. Increased expression of the HER-2 protein was noticed in cases of moderate (grade II) and poor (grade III) differentiation of carcinoma, as well as in cases where there was no metastases in the regional lymph nodes. No relationship has been observed between the expression of HER-2 and occurrence of vascular invasion. In cases of ductalinfiltrative breast cancer the expression of HER-2 protein is in correlation with the size and degree of tumor's differentiation, as well as with the presence of metastases in regional lymph nodes.
Association of Basic Medical Sciences of FBIH
2005-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3327
10.17305/bjbms.2005.3327
Biomolecules and Biomedicine; Vol. 5 No. 1 (2005); 3-7
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3327/945
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3328
2020-07-28T11:28:31Z
bjbms:PAT
driver
Histological changes of the sciatic nerve in dogs after intraneural application of lidocaine--relation to the established application pressure
Mornjaković, Zakira
Dilberović, Faruk
Ćosović, Esad
Divanović, Kučuk-Alija
Začiragić, Asja
Kapur, Eldan
Vučković, Ilvana
intraneural application of local anesthetic
application pressure
histology
dog
Histological changes of sciatic nerve in adult dogs 7 days after single application of 2% lido-caine (4 ml dose, speed of injection 3 ml/min) and measurement of the application pressure was studied, with a goal to investigate structural changes of the nerve in relation to the established pressure values. The application pressure was determined by using Bio Bench software. In intrafascicular puncture an average application pressure of 198.23 ± 52 kPa was found, and in interfascicular puncture its average value was 53.3 ± 17.9 kPa, with a note that individual differences are regularly present. Seven days after the injection, a nerve dissection was performed and serial sections covering the region of injection’s puncture and bordering proximal and distal zones, in the total length of 3 cm, were prepared. The found changed show the presence of nerves’ fibers lesions with a strong reactivity of Schwann’s cell, as well as the change of interstitial structure concerning hypercellularity and occurrence of cellular extravasation. The covering system of the nerve in the zone of epineurium manifests changes of inflammatory process and in perineurium a decomposition of lamella layers and the alteration of their tinctorial properties were noticed. A comparison of the found nerve reactivities in intra- and interfascicular application showed their one-way alteration, although the lesions were more noticeable in the conditions of intrafascicular application. The damages were mostly expressed in the zone of local application of anesthetic, than distally from it, while the damage to the structure in the proximal part is of the smallest degree.
Association of Basic Medical Sciences of FBIH
2005-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3328
10.17305/bjbms.2005.3328
Biomolecules and Biomedicine; Vol. 5 No. 1 (2005); 8-13
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3328/946
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3343
2021-01-09T13:55:41Z
bjbms:PAT
driver
Histological observations on adipocere in human remains buried for 21 years at the Tomašica grave-site in Bosnia and Herzegovina
Salihbegović, Adis
Clark, John
Sarajlić, Nermin
Radović, Svjetlana
Finlay, Finlay
Jogunčić, Anes
Spahić, Emina
Tuco, Vedo
Tomašica mass grave
adipocere
histopathology
The Tomašica grave-site near Prijedor in the north of Bosnia is reported to be the largest primary mass grave discovered thus far relating to the 1992–95 war. A total of 275 complete bodies and 125 body parts were exhumed from it in 2013. Post mortem examinations of the victims showed that nearly all had died from gunshot injuries but an additional striking feature was the degree of preservation of many of the bodies, even 21 years on, with skin, soft tissues and internal organs still present in abundance and gross structures clearly identifiable. Histology was performed on 68 samples of soft tissue from a total 13 bodies, on both skin and internal organs, and the degree of preservation was assessed in terms of the ability to recognize microscopic structure. Further comparison was made with samples taken a month or so later (56 tissue samples from 9 bodies, all but one different from the first group), after the bodies had been covered in salt as a means of general preservation. Generally, at a microscopic level, skin and subcutaneous tissues were better preserved than internal organs, while tissues sampled at the time of autopsy were better preserved than those sampled weeks later.
Association of Basic Medical Sciences of FBIH
2018-08-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3343
10.17305/bjbms.2018.3343
Biomolecules and Biomedicine; Vol. 18 No. 3 (2018); 234-239
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3343/1046
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3343/1841
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3352
2020-07-28T11:45:49Z
bjbms:PAT
driver
The Bcl-2 protein: a prognostic indicator strongly related to ER and PR in breast cancer
Bilalović, Nurija
Vranić, Semir
Hasanagić, Senad
Basić, Hiba
Tatarević, Aida
Bešlija, Semir
Selak, Ivan
bcl-2
ER
PR
breast cancer
estrogen
progesterone
Bcl-2, the protein product of the Bcl-2 gene, is a member of the Bcl-2 family of proteins that play a crucial role in a complex mechanism of apoptosis. It was recently proposed that bcl-2 could inhibit cancer progression. In this study, we evaluated the expression patterns of Bcl-2, estrogen receptors (ER), progesterone receptors (PR) in 71 primary invasive breast carcinomas and their association with other clinicopathological parameters. Samples from 71 patients with invasive breast cancer with follow-up ranging from 4-103 months (median 57 months) were included in the study. Forty-six patients (66%) obtained a complete response, while 5 (9%) were considered non-responders during the follow up period of 103 months. Eighteen (25%) patients died, 15 (21%) from primary disease and 3 (4%) from other disease. In unvaried analysis, tumor size (<2 cm), lymph node (<4 lymph nodes), hormonal status and Bcl-2 expression are correlated with longer overall (OS) and relapse-free survival (RFS). Patients with 4 or more positive axillary lymph nodes had significantly shorter OS (p=0.01) and RFS (p=0.009). Higher expression of Bcl-2 was associated with longer OS (p=0.02) and RFS (p=0.03), and this result were independent of axillary lymph nodes and tumor size in Cox multivariate analysis.
Association of Basic Medical Sciences of FBIH
2004-11-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3352
10.17305/bjbms.2004.3352
Biomolecules and Biomedicine; Vol. 4 No. 4 (2004); 5-12
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3352/959
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3375
2020-07-28T12:32:35Z
bjbms:PAT
driver
Development of the rat telencephalon-volumetric analysis
Aličelebić, Selma
Grbeša, Đurđica
rat telencephalon
development
stereology
With regard to intensive morphometric changes, morphometry as a method is mainly used for histogenetic studies of brain development in normal and experimental conditions. The aim of our study was to quantitatively analyse morphological parameters of the rat telencephalon during embryonic development. The investigation was carried out on semithin serial sections of rat brain from embryonic days 12 to 15. The volume densities (VV) of the lateral ventricles, the telencephalic neuroepithelium and the surrounding mesenchyme have been analysed stereologically and compared in examined embryonic stages. The neuroepithelial volume density was the smallest (28%) at E13 and the biggest (44%) at E15 (p<0.0005). The mesenchymal volume density was the smallest (32%) at E13 and the biggest (48%) at E14 (p<0.0005). The volume density of lateral ventricles was the biggest (40%) at E13 and the smallest (14%) at E15 (p<0.0005). Neurostereological methods have been making a very valuable contribution to neuroscience over recent years. We have used unbiased stereological counting methods to obtain objective quantitative parameters which show relations between some parts of rat embryonic telencephalon examined during its normal development.
Association of Basic Medical Sciences of FBIH
2004-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3375
10.17305/bjbms.2004.3375
Biomolecules and Biomedicine; Vol. 4 No. 3 (2004); 11-14
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3375/977
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3380
2020-07-28T12:33:19Z
bjbms:PAT
driver
Anti-cytokeratin 7: a positive marker for epithelial dysplasia in flat bowel mucosa
Radović, Svjetlana
Selak, Ivan
Babić, Mirsad
Vukobrat-Bijedić, Zora
Knežević, Željka
cytokeratin
dysplasia
colon mucosa
The aim of this paper is to establish by immunohistochemistry the expression of keratin 7 in inflammatory-regenerative flat bowel mucosa and in different grades of epithelial dysplasia regarding the sub-units expressed in normal and carcinomatous colonic mucosa. Biopsy specimens from 270 patients were examined: 74 were classified as inflammatory-regenerative changes and 196 as dysplastic lesions. There were 108 cases of mild dysplasia, 58 cases of moderate and 30 cases of severe dysplasia, respectively). Demonstration of location and intensity of cytokeratin 7 staining was performed by immunohistochemistry using monoclonal antibody (anti-cytokeratin 7). Findings of cytokeratin 7 in dysplastic lesions were compared with those in normal mucosa, inflammatory -regenerative mucosa and adenocarcinoma. Cytokeratin 7 is not found in normal colonic mucosa. In inflammatory-regenerative mucosa it was found in solitary cells in small number of cases. It is found in all cases of epithelial dysplasia and its expression showed no difference regarding moderate and severe dysplasia. In few cases of adenocarcinoma, cytokeratin 7 is found in traces and showed minimal staining intensity. Having in mind that cytokeratine 7 is primarily found in dysplastic lesions of the flat colonic mucosa it can be a valuable diagnostic tool in the histological interpretation of epithelial dysplasia.
Association of Basic Medical Sciences of FBIH
2004-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3380
10.17305/bjbms.2004.3380
Biomolecules and Biomedicine; Vol. 4 No. 3 (2004); 24-30
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3380/979
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3455
2020-07-29T09:05:19Z
bjbms:PAT
driver
Pathohistological changes in diffuse coronary atherosclerosis and chronic infection caused by Chlamydia pneumonia.
Legan, Mateja
Vraspir-Porenta, Olga
Kese, Darja
Zorc-Plesković, Ruda
Zorc, Marjeta
pathohistological changes
diffuse coronary atherosclerosis
chronic infection
Chlamydia pneumonia
BACKGROUND AND PURPOSE: To investigate the histopathologic characteristics of atherosclerotic lessions in diffuse coronary artery disease and to evaluate the possible inflammatory role of chronic infection with Chlamydia pneumoniae (CP).
MATERIALS AND METHODS: For 10 patients (males, mean age 61 years) who were surgically treated for grave diffuse coronary artery disease, histomorphological analyses of endarterectomized segments of the coronary arteries were performed. Serological analyses for the detection of CP antibodies in peripheral blood were done, preoperatively.
RESULTS AND CONCLUSIONS: Diffuse and concentric atherosclerotic changes from VI to VIII stage according to the Stary classification were found. Immunohistochemical methods revealed infiltrates of T-lymphocytes (80% of cases), B-lymphocytes (40% of cases) and macrophages (80%). Using the nuclear marker for proliferation activity MIB-1, single MIB-1 positive cells were found in 40% of cases. Features of arteriologenesis and vasculitis of newly formed arterioles (as well as thickening of the wall of newly formed arterioles) were found in the vessel wall of 8 patients, 7 of them had chronic infection with CP (preoperative micro-immunofluorescent test results: 1:32<IgG ≥1:512 and IgA≥32), one had passed CP infection (1:32 ≤IgG<1:512, IgA negative). These features were absent in 2 patients, both recovered from CP infection and had not the chronic CP infection at the time of surgery. DNA of Chlamydia pneumoniae was detected using the polymerase chain reaction (PCR) method in the vessel wall of 3 patients who were chosen randomly for this method. This study suggests an inflammatory and proatherogenic role of CP in a high grade atherosclerotic coronary artery wall in diffuse coronary artery disease.
Association of Basic Medical Sciences of FBIH
2004-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3455
10.17305/bjbms.2004.3455
Biomolecules and Biomedicine; Vol. 4 No. 1 (2004); 19-22
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3455/1012
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3560
2020-12-20T15:17:26Z
bjbms:PAT
driver
p16/Ki-67 dual staining has a better accuracy than human papillomavirus (HPV) testing in women with abnormal cytology under 30 years old
Pirtea, Laurențiu
Secosan, Cristina
Margan, Madalin
Moleriu, Lavinia
Balint, Oana
Grigoras, Dorin
Sas, Ioan
Horhat, Florin
Jianu, Adelina
Ilina, Răzvan
Human papillomavirus
immunocytochemistry
p16/Ki-67 dual staining
HPV genotyping
Due to a high rate of transient human papillomavirus (HPV) infection, HPV genotyping has a low specificity for high-grade cervical lesions, especially in young women. p16/Ki-67 dual immunocytochemical staining can also be used for the detection of oncogenic changes in cervical cells. Our aim was to compare the performance of p16/Ki-67 dual staining and HPV genotyping in the detection of high-grade cervical lesions in patients with atypical squamous cells of undetermined significance (ASCUS)/low-grade squamous intraepithelial lesion (LSIL) on Pap smear. We retrospectively analyzed 310 patients with ASCUS/LSIL on Pap smear, who underwent colposcopy. Among these, 161 patients with suspected lesions detected by colposcopy were referred for biopsy. HPV genotyping by LINEAR ARRAY HPV Genotyping Test (CE-IVD) and p16/Ki-67 dual staining by CINtec PLUS Cytology kit was performed prior to cervical biopsy. The overall sensitivity and specificity of HPV genotyping for the detection of cervical intraepithelial neoplasia (CIN) 2-3 was 79% and 72%, respectively in patients with ASCUS, and 85% and 64%, respectively in patients with LSIL. For p16/Ki-67 test, sensitivity and specificity rate was 66% and 93%, respectively in ASCUS and 59% and 79%, respectively in LSIL group. The specificity of p16/Ki-67 staining was significantly higher in both groups in patients aged <30 years compared to patients >30 years old (p < 0.001). Our results showed that p16/Ki-67 dual staining has a higher specificity compared to HPV genotyping, especially in patients under 30 years old. This indicates the usefulness of p16/Ki-67 testing in the triage of patients with ASCUS/LSIL and <30 years old, prior to referral for colposcopy and biopsy.
Association of Basic Medical Sciences of FBIH
2019-11-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3560
10.17305/bjbms.2018.3560
Biomolecules and Biomedicine; Vol. 19 No. 4 (2019); 336-341
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3560/1104
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3560/1747
Copyright (c) 2019 Association of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3567
2020-07-30T10:47:35Z
bjbms:PAT
driver
Type IV collagen immunoreactivity of basement membrane in inflammatory-regenerative and dysplastic lesions of the flat colonic mucosa
Radović, Svjetlana
Selak, Ivan
Babić, Mirsad
Knežević, Željka
Vukobrat-Bijedić, Zora
collagen IV
epithelial dysplasia
colon mucosa
The aim of this research is to establish by immunohistochemistry if there is a change in the expression of collagen type IV, as a substitute of basement membrane, in development of epithelial dysplasia in chronically inflamed colon mucosa.
Methods. Biopsy specimens from 270 patients were examined: 74 were classified as inflammatory-regenerative and 196 as dysplastic lesions. There were 108 cases of mild dysplasia, 58 cases of moderate and 30 cases severe dysplasia, respectively. Visualisation of collagen IV and its way of expression within basement membrane of glandular crypts was performed by immunohistochemistry and then compared with findings in normal colon mucosa and colon adenocarcinoma tissue.
Results. Changes in the expression of collagen IV comprised of its focal irregularities, diffuse thinning and/or thickening, focal interruptions or its complete absence. Significant changes in the expression of collagen IV in relation to normal mucosa already occur in inflammatory-regenerative mucosa. In mild dysplasia, these changes are more intensive in relation to those in inflammatory altered mucosa as well as at severe dysplasia in relation to moderate dysplasia. Changes in the expression of collagen IV in severe dysplasia are significantly more serious than in moderate dysplasia but are identical to those in colon adenocarcinoma tissue.
Conclusion. These findings suggest that change in the expression of collagen IV is in correlation to a degree of epithelial dysplasia that developed in flat chronically inflamed colon mucosa.
Association of Basic Medical Sciences of FBIH
2003-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3567
10.17305/bjbms.2003.3567
Biomolecules and Biomedicine; Vol. 3 No. 1 (2003); 30-35
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3567/1075
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3568
2020-07-30T10:48:58Z
bjbms:PAT
driver
Proximal convoluted tubules of the rats kidney--a stereological analysis
Aličelebić, Selma
kidney
proximal convoluted tubule
stereology
rat
Background and Purpose: The aim of this work was to order quantitative parameters of the proximal convoluted tubules (PCT) in the normal rat kidney cortex. Volume density (VV), both surface and specific surface density toward interstitium (SVi and SVi/VV) and toward lumen (SVl and SVl/VV) and thickness (T) of tubules epithelium have been stereologically ordered.
Material and Methods: Stereologically were analysed 170 test fields by lattice L36 on the paraffin sections of the three adult male Wistar rats kidney dyeing by PAS-method.
Results: The mean values of the variables analysed were Vv=76.4% ±0.012; Svi=0.056pm-1 ±0.004; Svl=0.028pm-1 ±0.003; Svi/Vv=0.073pm-1 ±0.003; Svl/Vv=0.037pm-1 ±0.005; T=18,26pm ±0.897.
Conclusions: Stereological methods are making a very valuable contribution to science over recent years. We have used unbiased stereological counting methods to obtain objective quantitative parameters of the PCT epithelium in the normal rats’ kidney cortex.
Association of Basic Medical Sciences of FBIH
2003-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3568
10.17305/bjbms.2003.3568
Biomolecules and Biomedicine; Vol. 3 No. 1 (2003); 36-39
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3568/1076
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3605
2020-07-30T11:01:45Z
bjbms:PAT
driver
Stereological Analysis of the Mammary Gland in Primiparous Lactating Rats during the Lead Acetate Intoxication
Mornjaković, Zakira
Kadić, Muhamed
Stereological Analysis
Mammary Gland
Acetate Intoxication
The present study was undertaken to investigate the changes in composition of the mammary gland volume unit through all phases of lactation in rats subjected to lead acetate administration via drinking water during the first pregnancy and lactation. Stereological analysis was performed on serial slices of the organs. The point of support for our study was the fact that lead, apart from being a poison of wide range, is mostly poisonous for gonads and to their supervisory neuroendocrine structures and the mammary gland, in morpho-functional sense, should be admitted as their integral part.
On the 7th day of lactation there was significant difference of the alveolar and ductal epithelium phase, which was significantly reduced in animals treated by lead. In the same animals there were larger lumens of the alveoli and ducti, more abundant connective tissue and greater number of the adipocytes but these differences in comparison to control group were not significant.
On the 14th day of lactation there was significantly greater presence of the adipocytes phase and stromal tissue while the volume of the epithelium of alveoli and ducti was significantly reduced in study group. There was also an increase of the alveoloductal lumen phase but this was not significant.
On the 21st day of lactation there were significant difference in epithelial and stromal tissue phases in two groups, having a significant decrease in the epithelium, and significant increase of stromal tissue in glands of lactating rats treated by lead acetate. There were non-significant differences as far as the presence of the adipocytes and alveoloductal lumen volume were concerned although the values for both phases in study group were above the control values.
On the basis of given results we concluded that lead changes the quantitative characteristics of the mammary gland, i.e. the composition of the volume unit of the organ through all phases of lactation.
Association of Basic Medical Sciences of FBIH
1998-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3605
10.17305/bjbms.1998.3605
Biomolecules and Biomedicine; Vol. 1 No. 1 (1998); 4-7
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3605/1096
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3605/1254
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3607
2020-07-30T11:02:20Z
bjbms:PAT
driver
Local Cellular Immune Response in Dysplastic Epithelial Lesions of the Flat Colonic Mucosa
Radović, Svjetlana
Selak, Ivan
Bratović, I.
immunocompetent cells
epithelial dysplasia
immunological techniques
intestinal neoplasia
This is an immunohistochemical study of the local cellular immune response characteristic in inflammatory-regenerative and dysplastic flat colonic mucosa. The aim of this study is to determine a possible existence of the mononuclear cellular infiltration specificity which could be important for the prognosis in further development of dysplastic lesion. Biopsy specimens from 170 patients (specimens stained by hematoxylin eosin) were examined. 74 specimens showed inflammatory-regenerative changes and 96 had dysplastic changes (38 with mild dys-plasia, 28 with moderate dysplasia and 30 with severe dysplasia). Three monoclonal antibodies were used for the identification of mononuclear cells in the inflammatory cellular infiltration in the lamina propria of colonic mucosa. The inflammatory cells type and their location in respect of the epithelial cells and lesion itself were analysed and their number was determined by the semi-quantitative method. T lymphocytes were the dominant cells of local immune response in dysplastic lesions while macrophages were less present and B lymphocytes, as rare cells, were present in sporadic cases. It is notified that increase in the extent of dysplasia was followed by increase in the number of macrophages and T lymphocytes in particular. Immediate contact between macrophages and T lymphocytes in epithelial dysplasia was found in a small number of cases and was mostly independent from the intensity of dysplasia. Signs of the direct lytical effect of the mononuclear ceils on dysplastic epithelial cells were not observed in this contact. It seems that epithelial dysplasia does not provoke more significant local immune response which is the most probably a part of the chronic non-specific inflammation that has a negative influence on further development of the lesion. The conclusion is that local immune response in the dysplastic alteration of flat colonic mucosa has no importance in further prognosis of the lesion.
Association of Basic Medical Sciences of FBIH
1998-02-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3607
10.17305/bjbms.1998.3607
Biomolecules and Biomedicine; Vol. 1 No. 1 (1998); 12-19
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3607/1098
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3607/1256
Copyright (c) 2018 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/3970
2020-11-29T16:29:38Z
bjbms:PAT
driver
Internodal HER2 heterogeneity of axillary lymph node metastases in breast cancer patients
Baroš, Ilija Vladimir
Tanasković, Nataša
Pellas, Ulrika
Eri, Živka
Tadić Latinović, Ljiljana
Tot, Tibor
Breast Cancer
HER2
gene protein assay
GPA
tumoral heterogeneity
lymph nodes
metastasis
Determination of human epidermal growth factor receptor 2 (HER2) status is important for adequate treatment of breast cancer (BC) patients. The novel HER2 gene protein assay (GPA) is particularly convenient, as it allows the simultaneous assessment of HER2 protein expression and gene amplification at individual cell level. Here we investigated the frequency of internodal HER2 heterogeneity in axillary lymph node macrometastases of BC patients and compared HER2 status between primary breast tumor and its metastases. We included a total of 41 female patients operated between 2014 and 2015 for primary BC with axillary lymph node macrometastases. Representative paraffin blocks of metastatic lymph nodes were sectioned and the slides were stained using the GPA in 38 BC cases. GPA results were assessed according to the ASCO/CAP 2013 criteria. We analyzed 12586 individual tumor cells, 120 cells per section of each metastatic lymph node. HER2 status differed between the primary tumor and its metastases in 5/38 cases (13.2%). In patients with at least two metastatic nodes, the HER2 status of lymph node metastases was only slightly different in 4/23 cases (17.4%). Our results indicate rare but substantial differences in HER2 status between primary breast tumor and its axillary lymph node metastases that may direct the choice and outcomes of targeted therapy in BC patients. The impact of the rare and subtle internodal HER2 heterogeneity evidenced in this study remains uncertain. Determining the HER2 status of lymph node metastases in BC seems to be rational, but assessing a limited number of metastatic nodes may be sufficient.
Association of Basic Medical Sciences of FBIH
2019-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3970
10.17305/bjbms.2019.3970
Biomolecules and Biomedicine; Vol. 19 No. 3 (2019); 242-248
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3970/1217
https://www.bjbms.org/ojs/index.php/bjbms/article/view/3970/1675
Copyright (c) 2019 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/4131
2020-11-29T16:31:56Z
bjbms:PAT
driver
Expression patterns and prognostic value of miR-210, miR-494, and miR-205 in middle-aged and old patients with sepsis-induced acute kidney injury
Lin, Yongjun
Ding, Ying
Song, Shuping
Li, Man
Wang, Tao
Guo, Feng
microRNA
Sepsis
Acute kidney injury
Prognosis
Predictive value
Septic patients suffer a ‘cytokine storm’ from proinflammatory cytokines, chemokines and other inflammatory mediators, resulting in acute kidney injury (AKI) and death. The purpose of the present study was to determine the expression patterns of microRNA-210 (miR-210), miR-494, and miR-205 in middle-aged and old patients with sepsis-induced AKI and to evaluate their association with patient prognosis. Serum blood urea nitrogen (BUN), creatinine (Cr) and cystatin C levels were determined in peripheral venous blood collected from 110 patients with sepsis-induced AKI and 110 healthy controls. The expression profile of 30 miRNAs was analyzed by TaqMan low-density array (TLDA) in plasma samples from patients and controls. Association of miRNAs with prognosis and survival of patients was analyzed by Spearman’s rank correlation coefficient, Cox multivariate analysis, and ROC curve analysis. TILDA analysis showed 11 upregulated and 11 downregulated miRNAs in patients with sepsis-induced AKI. MiR-210 and miR-494 were the most upregulated and miR-205 was the most downregulated miRNA. High expression of miR-210 and miR-494 was positively correlated with BUN, Cr and cystatin C levels of patients, while low expression of miR-205 was negatively correlated. MiR-210 and miR-494 expression was significantly decreased and miR-205 expression was increased in survivors with sepsis-induced AKI (28-day survival, n = 68) vs. non-survivors (n = 42). BUN, Cr, and miR-205 were independent risk factors for prognosis in sepsis-induced AKI. Our study showed the predictive value of miR-210, miR-494, and miR-205 in prognosis and survival of patients with sepsis-induced AKI. MiR-205 is an independent risk factor for sepsis-induced AKI and its decreased expression is associated with shorter patient survival.
Association of Basic Medical Sciences of FBIH
2019-08-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4131
10.17305/bjbms.2019.4131
Biomolecules and Biomedicine; Vol. 19 No. 3 (2019); 249-256
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4131/1223
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4131/1676
Copyright (c) 2019 Bosnian Journal of Basic Medical Sciences
oai:ojs2.www.bjbms.org:article/4281
2020-12-20T14:40:42Z
bjbms:PAT
driver
Expression of the anti-Mullerian hormone, kisspeptin 1, and kisspeptin 1 receptor in polycystic ovary syndrome and controlled ovarian stimulation rat models
Risvanli, Ali
Ocal, Halis
Timurkaan, Necati
Ipek, Pinar
Seker, Ibrahim
Karabulut, Burak
Anti-Mullerian hormone
kisspeptin
ovarian stimulation
polycystic ovary syndrome
COS
PCOS
KISS-1
KISS1r
GPR54 receptor
Polycystic ovary syndrome represents a significant cause of female infertility. The aim of this study was to investigate the expression of anti-Mullerian hormone (AMH), kisspeptin 1 (KISS-1), and kisspeptin 1 receptor (KISS1r) in rat models of polycystic ovary syndrome (PCOS) and controlled ovarian stimulation (COS). For this purpose, 28 rats were assigned into four groups. Estrus and Diestrus groups consisted of rats in estrus and diestrus phases, respectively, while COS and PCOS groups consisted of rats with induced COS and PCOS, respectively. The serum AMH, KISS-1, and estradiol levels, and ovarian KISS1r levels were analyzed by enzyme-linked immunosorbent assay. Furthermore, histopathological analysis of the ovary tissue was done and ovarian KISS-1 expression was determined by immunohistochemical assay. The results revealed that ovarian KISS1r levels were higher in the Estrus (1271.43±51.98 pg/mL) and COS (1191.43±85.67 pg/mL) groups, compared to Diestrus and PCOS groups. The highest level of AMH was found in the Estrus group (16.91±2.12 ng/mL). The results indicate that AMH had no effect on the development of COS and PCOS, while KISS-1 was found to affect the development of COS in rats.
Association of Basic Medical Sciences of FBIH
2020-02-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4281
10.17305/bjbms.2019.4281
Biomolecules and Biomedicine; Vol. 20 No. 1 (2020); 37-43
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4281/1288
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4281/1730
Copyright (c) 2019 The Authors
oai:ojs2.www.bjbms.org:article/4409
2020-12-19T21:06:44Z
bjbms:PAT
driver
Inflammatory cells in perivascular adipose tissue and the integrity of the tunica media in atherosclerotic coronary arteries
Zorc-Pleskovič, Ruda
Zorc, Marjeta
Šuput, Dušan
Milutinović, Aleksandra
Perivascular adipose tissue
PVAT
atherosclerosis
coronary arteries
endarterectomy
CAD
coronary artery disease
inflammatory infiltration
Obstructive coronary artery disease (CAD) is characterized by inflammation within the atherosclerotic coronary arteries. Infiltration of inflammatory cells into muscular media can lead to remodeling and weakening of the arterial wall. We examined the relationship between inflammatory infiltration in perivascular adipose tissue (PVAT), state of the external elastic membrane, and the intensity of inflammatory infiltration in the tunica media of coronary arteries obtained by endarterectomy from symptomatic patients with diffuse CAD. We analyzed endarterectomy sequesters from 22 coronary arteries that contained the intima, media, a part of the adventitia, and PVAT in at least one part of the sequester. The coronary arteries were divided into two groups according to the presence or absence of inflammatory infiltration in PVAT. Staining with hematoxylin-eosin and by the Movat's method showed atherosclerotic changes in the intima and media. Immunohistochemistry (anti-leukocyte common antigen [LCA] antibody) was used for the detection of leukocytes. We found a significant positive correlation between inflammatory infiltration in PVAT and preservation of the external elastic membrane of coronary arteries. Furthermore, we found a significant negative correlation between inflammatory infiltration in PVAT and the intensity of inflammatory infiltration in the media. It seems that the integrity of the external elastic membrane and the proinflammatory properties of PVAT restrain inflammatory cells within PVAT. Both effects may prevent the migration of inflammatory cells into the media and delay the development of CAD.
Association of Basic Medical Sciences of FBIH
2020-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4409
10.17305/bjbms.2019.4409
Biomolecules and Biomedicine; Vol. 20 No. 2 (2020); 183-187
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4409/1277
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4409/1718
oai:ojs2.www.bjbms.org:article/4471
2021-01-15T18:08:31Z
bjbms:PAT
driver
Inflammation-related cytokines and their roles in gastroenteropancreatic neuroendocrine neoplasms
Herman Mahečić, Davorka
Cigrovski Berković, Maja
Zjačić-Rotkvić, Vanja
Čačev, Tamara
Kapitanović, Sanja
Ulamec, Monika
Inflammatory microenvironment
TNF-α
IL-6
IL-1β
IL-2
GEP-NEN
Proinflammatory counterworks are important at different stages of tumor development, particularly during invasion and metastasis. Immune cells and their signal molecules can influence all stages of tumor progression, as well as therapeutic intervention. Proinflammatory cytokines are known triggers of growth in gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs). In this study, we explored the immunohistochemical expression of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), IL-2, and IL-6 in tissues from 43 GEP-NEN patients with tumors of gastric, duodenal, ileal, appendiceal, and colonic origin. The immunohistochemical expression of TNF-α was increased in tumor groups with high proliferation rates (Ki-67; p = 0.034), as well as in those with higher tumor grades (p = 0.05). Moreover, the immunohistochemical expression of TNF-α positively correlated with death outcomes (p = 0.016). Expression of IL-6, IL-1β, and IL-2 displayed similar immunohistochemical expression patterns regardless of Ki-67, although the expression between the ILs differed. Most GEP-NENs had high levels of IL-6 and lower levels of IL-1β and IL-2. Although further comprehensive studies are required for a complete understanding of activated mechanisms in proinflammatory protumoral microenvironment of GEP-NENs, TNF-α is a potential marker in the prognosis of those tumors.
Association of Basic Medical Sciences of FBIH
2020-11-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4471
10.17305/bjbms.2020.4471
Biomolecules and Biomedicine; Vol. 20 No. 4 (2020); 445-450
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4471/1314
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4471/1635
Copyright (c) 2020 Davorka Herman Mahečić, Maja Cigrovski Berković, Vanja Zjačić-Rotkvić, Tamara Čačev, Sanja Kapitanović, Monika Ulamec
oai:ojs2.www.bjbms.org:article/4474
2020-11-23T23:25:34Z
bjbms:PAT
driver
Concordance of PD-L1 expression and CD8+ TIL intensity between NSCLC and synchronous brain metastases
Batur, Sebnem
Dulger, Onur
Durak, Sermin
Yumuk, Perran Fulden
Caglar, Hale Basak
Bozkurtlar, Emine
Bozkurt, Suheyla
Tastekin, Ebru
Cicin, Irfan
Ahiskali, Rengin
Rzazade, Rashad
Cakir, Asli
Oz, Buge
Programmed death-ligand 1
non-small-cell lung cancer
NSCLC
tumor immunology
PD-L1
brain metastasis
CD8 lymphocytes
tumor infiltrating lymphocytes
TILs
Programmed death-ligand 1 (PD-L1) is suggested to be a predictive biomarker in non-small-cell lung carcinoma (NSCLC). However, the differential expression of PD-L1 in primary lung tumor vs. synchronous metastases, especially brain metastasis (BM), remains unclear. This study assessed the concordance of PD-L1 expression on tumor cells and tumor-infiltrating lymphocytes (TILs) and CD8+ TIL intensity between primary lung tumors and synchronous BMs from 24 NSCLC patients. PD-L1, CD3, and CD8 positivity was determined by immunohistochemistry (IHC). PD-L1 scoring was based on the proportion of tumor cells with membranous expression of PD-L1 and the cutoff values <1%, 1–49%, and ≥50%. CD3 and CD8 positivity in TILs was evaluated semi-quantitatively and the proportion of CD3+/CD8+ TILs was determined. PD-L1 expression on tumor cells and TILs was evaluated in relation to CD3+/CD8+ TIL proportions and the intensity of CD8+ TILs between the paired primary lung and BM tissues. In the primary lung tumors, PD-L1 positivity was observed in 25%, 37.5%, and 37.5% cases for the cutoff values <1%, 1–49%, and ≥50%, respectively. PD-L1 expression on tumor cells was strongly correlated between the paired primary lung and BM tissues, in all cutoff groups. However, PD-L1 expression on TILs and the proportion of CD3+/CD8+ TILs were not strongly correlated in all three groups between the paired primary lung tumors and BMs. The intensity of CD8+ TILs was concordant in only 54.16% of the paired primary lung tumors and BMs. This study showed a high concordance of PD-L1 expression in neoplastic cells between primary NSCLC and synchronous BMs.
Association of Basic Medical Sciences of FBIH
2020-08-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4474
10.17305/bjbms.2019.4474
Biomolecules and Biomedicine; Vol. 20 No. 3 (2020); 329-335
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4474/1292
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4474/1656
Copyright (c) 2020 Sebnem Batur, Onur Dulger, Buge Oz
oai:ojs2.www.bjbms.org:article/4485
2021-11-23T09:21:23Z
bjbms:PAT
driver
Cisplatin inhibits the proliferation of Saos-2 osteosarcoma cells via the miR-376c/TGFA pathway
Wang, Yuan
Wu, Yichao
Cai, Awei
Ma, Chengxiao
Cai, Shang
Wang, Hao
Que, Yukang
Xu, Shenglin
Xu, Tangbing
Hu, Yong
Cisplatin
miR-376c
transforming growth factor alpha
TGFA
Saos-2
cell proliferation
cell growth
osteosarcoma
The transforming growth factor alpha (TGFA) gene is involved in the proliferation and metastasis of various tumors, but its role in cell sensitivity to cisplatin chemotherapy is unclear. In this study, we investigated the mechanism underlying inhibitory effects of cisplatin on growth and proliferation of osteosarcoma cells. Osteosarcoma and normal skeletal muscle tissues were collected from 26 patients by biopsy. TGFA was silenced or overexpressed in Saos-2 osteosarcoma cells by transfection with TGFA-shRNA or TGFA ORF clone, respectively. MiR-376c was inhibited or overexpressed by transfection of Saos-2 cells with miR-376c sponge or miR-376c mimics, respectively. Cell growth was analyzed by MTT assay and cell proliferation by BrdU assay. MiR-376c and TGFA mRNA expression was detected by quantitative reverse transcription PCR and TGFA protein expression by Western blot. The target relationship between miR-376c and TGFA was assessed by luciferase reporter assay. Both in osteosarcoma tissues and Saos-2 cells, miR-376c expression was significantly decreased and TGFA mRNA expression was significantly increased compared with control. Transfection of Saos-2 cells with TGFA-shRNA silenced TGFA expression and significantly inhibited cell growth and proliferation. TGFA mRNA and protein expression in Saos-2 cells significantly decreased with increasing cisplatin concentrations (2.5–10 mg/L). Transfection with TGFA ORF clone reversed the inhibitory effects of cisplatin on Saos-2 cell proliferation. Compared with cisplatin (10 mg/L) treatment alone, the combined treatment with cisplatin and miR-376c mimics inhibited the proliferation of Saos-2 cells more significantly. MiR-376c suppressed TGFA expression by directly interacting with its 3' UTR region. Overall, cisplatin inhibited the proliferation of Saos-2 cells by upregulating miR-376c and downregulating TGFA expression.
Association of Basic Medical Sciences of FBIH
2021-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4485
10.17305/bjbms.2020.4485
Biomolecules and Biomedicine; Vol. 21 No. 2 (2021); 163-173
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4485/1303
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4485/2085
Copyright (c) 2020 Yuan Wang, Yichao Wu, Awei Cai, Chengxiao Ma, Shang Cai, Hao Wang, Yukang Que, Shenglin Xu, Tangbing Xu, Yong Hu
oai:ojs2.www.bjbms.org:article/4620
2020-12-19T21:08:54Z
bjbms:PAT
driver
P2X7 receptor as an independent prognostic indicator in gastric cancer
Calik, Ilknur
Calik, Muhammet
Sarikaya, Burcu
Ozercan, Ibrahim Hanifi
Arslan, Ramazan
Artas, Gokhan
Dagli, Adile Ferda
Gastric cancer
P2X7 receptor
survival
tumor-infiltrating lymphocytes
prognosis
Gastric cancer (GC) is one of the foremost causes of cancer-related death around the world. The P2X7 receptor (P2X7R), a member of the P2X7R subfamily of P2 receptors, is a unique molecule that has been shown to affect tumor growth and progression as well as various inflammatory processes, including proliferation of T lymphocytes, release of cytokines, and production of free oxygen radicals. P2X7R has been established as a prognostic parameter in some cancers, and recently, it has been investigated in the development of new targeted therapies. In the present study, we aimed to investigate the prognostic value of P2X7R expression in GC. The expression profile of P2X7R was evaluated immunohistochemically in 156 paraffin-embedded human GC specimens. P2X7R expression was higher in patients with lymph node metastasis than in those without (p < 0.001). P2X7R overexpression was closely related with tumor-infiltrating lymphocytes (TILs) (p = 0.001), vascular invasion (p = 0.006), depth of invasion (p < 0.001), distant metastasis (p < 0.001), and advanced tumor, node, metastasis stage (p < 0.001). Moreover, univariate (hazard ratio [HR] 3.98; 95% confidence interval (CI) 1.89-11.82; p < 0.001) and multivariate (HR 2.24; 95% CI 3.53-12.50; p < 0.001) Cox regression analysis showed that upregulated P2X7R expression clearly correlated with worsened overall survival. In summary, our data revealed that P2X7R may serve as a reliable prognostic parameter and promising therapeutic target for GC.
Association of Basic Medical Sciences of FBIH
2020-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4620
10.17305/bjbms.2020.4620
Biomolecules and Biomedicine; Vol. 20 No. 2 (2020); 188-196
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4620/1307
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4620/1719
Copyright (c) 2020 Ilknur Calik, Muhammet Calik, Burcu Sarikaya, Ibrahim Hanifi Ozercan, Ramazan Arslan, Gokhan Artas, Adile Ferda Dagli
oai:ojs2.www.bjbms.org:article/4632
2020-11-25T10:46:25Z
bjbms:PAT
driver
Significance of chromogranin A and synaptophysin in pancreatic neuroendocrine tumors
Tomita, Tatsuo
Chromogranin A
immunohistochemistry
multiple endocrine neoplasia 1
pancreatic neuroendocrine tumors
synaptophysin
The two most commonly used immunohistochemical markers for neuroendocrine cells and their tumors are chromogranin A (CgA) and synaptophysin (SPY). CgA is a marker for neuroendocrine secretory granules of four pancreatic hormones and gastrin while SPY is a marker for synaptic vesicles in neuroendocrine cells, which release classic neurotransmitters such as acetylcholine and others. CgA is involved in synthesis and secretion of peptide hormones through exocytosis while the function of SPY is elusive. Thirty-five pancreatic neuroendocrine tumors (Pan-NETs) were studied, consisting of 14 insulinomas, 8 gastrinomas, 2 glucagonomas, 6 pancreatic polypeptidomas and 5 non-functioning tumors, and were immunostained for four pancreatic hormones, gastrin, CgA, and SPY. Majority of Pan-NETs were less immunostained for the endocrine hormones and CgA than the normal pancreatic endocrine cells. CgA immunostaining mostly correlates with each hormone staining in non-β-cell tumors, while SPY immunostaining recognizes endocrine cells diffusely in the cytoplasm. CgA immunostaining is less in insulinomas than in non-β-cell tumors, and CgA immunostaining may distinguish CgA-weaker insulinomas from CgA-stronger non-β-cell tumors. CgA immunostaining may be used as an independent marker for biological aggressiveness in non-β-cell Pan-NETs. The serum CgA levels are higher in subjects harboring non-β-cell tumors than those harboring insulinomas, and the serum CgA elevates in parallel to the increasing metastatic tumor mass. Thus, CgA positive immunostaining in Pan-NETs correlates with the elevated serum levels of CgA for diagnosing CgA-positive non-β-cell Pan-NETs and the increasing serum CgA levels indicate increasing metastatic tumor mass.
Association of Basic Medical Sciences of FBIH
2020-08-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4632
10.17305/bjbms.2020.4632
Biomolecules and Biomedicine; Vol. 20 No. 3 (2020); 336-346
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4632/1304
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4632/1659
Copyright (c) 2020 Tatsuo Tomita
oai:ojs2.www.bjbms.org:article/4656
2021-01-15T18:08:31Z
bjbms:PAT
driver
Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery
Milutinović, Aleksandra
Zorc-Pleskovič, Ruda
Endothelial cells
saphenous vein
endoscopic harvesting
open harvesting
coronary artery bypass grafting
The patency of the vein graft in coronary artery bypass grafting could be dependent on the conventional open (vsO) or endoscopic (vsE) harvesting and on the hypoxic damage of endothelial cells. We aimed to evaluate both surgical techniques according to endothelial loss that occurs in the time between harvesting and implantation. Twenty-six saphenous veins were divided into vsO (n = 16) and vsE (n = 10) group. Three samples were taken from each vein. The first sample was taken after removal, the second before implantation of the distal part, and the third before the implantation of the proximal part, and they were stained with HE, Movat, and immunohistochemically with CD31. A significant loss of endothelial cells within both groups was found at the time of implantation of the distal and the proximal part of the vein graft compared to the endothelial cells at the time of harvesting. There were no significant differences in the endothelial loss between vsE and vsO groups at the time of harvesting and at the time before the implantation of the distal part. A higher number of endothelial cells was found in vsE group compared to vsO group at the time just before the implantation of the proximal part. The comparison of the implanted portions of vsE and vsO grafts to mammary arteries revealed a significant loss of endothelial cells only in vsO graft. We conclude that, at the time of implantation, the endothelial layer of the vein graft harvested endoscopically is more preserved than of the vein graft harvested openly.
Association of Basic Medical Sciences of FBIH
2020-11-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4656
10.17305/bjbms.2020.4656
Biomolecules and Biomedicine; Vol. 20 No. 4 (2020); 451-458
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4656/1316
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4656/1636
Copyright (c) 2020 Aleksandra Milutinović, Ruda Zorc-Pleskovič
oai:ojs2.www.bjbms.org:article/4823
2021-03-19T21:00:57Z
bjbms:PAT
driver
Dose-dependent effects of adalimumab in neonatal rats with hypoxia/reoxygenation-induced intestinal damage
Kocamaz, Halil
Özdemir, Özmert MA
Türk, Nilay Şen
Enli, Yaşar
Şahin, Barbaros
Ergin, Hacer
Adalimumab
hypoxia
intestine
reperfusion
tumor necrosis factor-alpha
Tumor necrosis factor-alpha (TNF-α) has an important role in hypoxia/reoxygenation (H/R)-induced intestinal damage. It was shown that blocking TNF-α with infliximab has beneficial effects on experimental necrotizing enterocolitis and hypoxic intestinal injury. However, there is no data about the effect of adalimumab on H/R-induced intestinal damage. Therefore, we aimed to determine potential dose-dependent benefits of adalimumab in such damage in neonatal rats. Wistar albino rat pups were assigned to one of the four groups: control group, hypoxia group, low-dose adalimumab (5 mg/kg/day) treated group (LDAT), and high-dose adalimumab (50 mg/kg/day) treated group (HDAT). On the fourth day of the experiment, all rats except for the control group were exposed to H/R followed by euthanasia. Malondialdehyde (MDA), myeloperoxidase (MPO), TNF-α, total antioxidant capacity (TAC), and total oxidant capacity (TOC) were measured in intestinal tissue. TAC and TOC values were used to calculate the oxidative stress index (OSI). Histopathological injury scores (HIS) were also evaluated in the tissue samples. MDA levels were significantly lower in the LDAT and HDAT groups (p < 0.001). TNF-α levels were significantly lower in the LDAT group (p < 0.001). OSI was significantly higher in the H/R group than in the control and LDAT groups (p < 0.001). Mean HIS values in the LDAT group were significantly lower than those in the H/R and HDAT groups (p < 0.001). This experimental study showed that low-dose adalimumab appears to have a beneficial effect on intestinal injury induced with H/R in neonatal rats.
Association of Basic Medical Sciences of FBIH
2021-02-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4823
10.17305/bjbms.2020.4823
Biomolecules and Biomedicine; Vol. 21 No. 1 (2021); 33-38
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4823/1353
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4823/1896
Copyright (c) 2020 Halil Kocamaz, Özmert MA Özdemir, Nilay Şen Türk, Yaşar Enli, Barbaros Şahin, Hacer Ergin
oai:ojs2.www.bjbms.org:article/4978
2021-11-19T18:25:23Z
bjbms:PAT
driver
Stomach-specific c-Myc overexpression drives gastric adenoma in mice through AKT/mammalian target of rapamycin signaling
Liu, Jing
Feng, Wenxin
Liu, Min
Rao, Hanyu
Li, Xiaoxue
Teng, Yan
Yang, Xiao
Xu, Jin
Gao, Weiqiang
Li, Li
c-Myc
gastric adenoma
transgenic
AKT/mammalian target of rapamycin
Gastric cancer (GC) is one of the most common malignant cancers in the world. c-Myc, a well-known oncogene, is commonly amplified in many cancers, including gastric cancer. However, it is still not completely understood how c-Myc functions in GC. Here, we generated a stomach-specific c-Myc transgenic mouse model to investigate its role in GC. We found that overexpression of c-Myc in Atp4b+ gastric parietal cells could induce gastric adenoma in mice. Mechanistically, c-Myc promoted tumorigenesis via the AKT/mTOR pathway. Furthermore, AKT inhibitor (MK-2206) or mTOR inhibitor (Rapamycin) inhibited the proliferation of c-Myc overexpressing gastric cancer cell lines. Thus, our findings highlight that gastric tumorigenesis can be induced by c-Myc overexpression through activation of the AKT/mTOR pathway.
Association of Basic Medical Sciences of FBIH
2021-08-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4978
10.17305/bjbms.2020.4978
Biomolecules and Biomedicine; Vol. 21 No. 4 (2021); 434-446
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4978/1854
https://www.bjbms.org/ojs/index.php/bjbms/article/view/4978/2338
Copyright (c) 2020 Jing Liu, Wenxin Feng, Min Liu, Hanyu Rao, Xiaoxue Li, Yan Teng, Xiao Yang, Jin Xu, Weiqiang Gao, Li Li
oai:ojs2.www.bjbms.org:article/5088
2021-11-23T09:22:13Z
bjbms:PAT
driver
The Ki-67 proliferation index predicts recurrence-free survival in patients with dermatofibrosarcoma protuberans
Adem, Deligonul
Yazici, Serkan
Ozsen, Mine
Cetintas, Sibel Kahraman
Yalcinkaya, Ulviye
Şahin, Ahmet Bilgehan
Tanrıverdi, Ozgur
Orhan, Sibel Oyucu
Ocak, Birol
Cubukcu, Erdem
Kahveci, Ramazan
Evrensel, Turkkan
Dermatofibrosarcoma protuberans
Ki-67 proliferation index
disease recurrence
prognosis
Dermatofibrosarcoma protuberans (DFSP) is an uncommon soft tissue sarcoma that originates from the dermis or subcutaneous tissue in the skin. While its prognosis is generally favorable, disease recurrence is relatively frequent. Because morbidity after repeated surgery may be significant, an optimized prediction of recurrence-free survival (RFS) has the potential to improve current management strategies. The purpose of this study was to investigate the prognostic value of the Ki-67 proliferation index with respect to RFS in patients with DFSP. We retrospectively analyzed data from 45 patients with DFSP. We calculated the Ki-67 proliferation index as the percentage of immunostained nuclei among the total number of tumor cell nuclei regardless of the intensity of immunostaining. We constructed univariate and multivariate Cox proportional hazards regression models to identify predictors of RFS. Among the 45 patients included in the study, 8 developed local recurrences and 2 had lung metastases (median follow-up: 95.0 months; range: 5.2−412.4 months). The RFS rates at 60, 120, and 240 months of follow-up were 83.8%, 76.2%, and 65.3%, respectively. The median Ki-67 proliferation index was 14%. Notably, we identified the Ki-67 proliferation index as the only independent predictor for RFS in multivariate Cox proportional hazards regression analysis (hazard ratio = 1.106, 95% confidence interval = 1.019−1.200, p = 0.016). In summary, our results highlight the potential usefulness of the Ki-67 proliferation index for facilitating the identification of patients with DFSP at higher risk of developing disease recurrences.
Association of Basic Medical Sciences of FBIH
2021-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5088
10.17305/bjbms.2020.5088
Biomolecules and Biomedicine; Vol. 21 No. 2 (2021); 174-178
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5088/1621
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5088/2086
Copyright (c) 2020 Deligonul Adem, Serkan Yazici, Mine Ozsen, Sibel Kahraman Cetintas, Ulviye Yalcinkaya, Ahmet Bilgehan Şahin, Ozgur Tanrıverdi, Sibel Oyucu Orhan, Birol Ocak, Erdem Cubukcu, Ramazan Kahveci, Turkkan Evrensel
oai:ojs2.www.bjbms.org:article/5142
2021-06-04T16:05:18Z
bjbms:PAT
driver
Raloxifene inhibits the overexpression of TGF-β1 in cartilage and regulates the metabolism of subchondral bone in rats with osteoporotic osteoarthritis
Ping, Shao-Hua
Tian, Fa-Ming
Liu , Hao
Sun, Qi
Shao, Li-Tao
Lian, Qiang-Qiang
Zhang, Liu
Osteoporosis
osteoarthritis
raloxifene
TGF-β1
cartilage
subchondral bone
Overexpression of transforming growth factor-beta 1 (TGF-β1) and subchondral bone remodelling play key roles in osteoarthritis (OA). Raloxifene (RAL) reduces the serum level of TGF-β1 in postmenopausal women. However, the effect of RAL on TGF-β1 expression in articular cartilage is still unclear. Therefore, we aimed to investigate the protective effect of RAL on osteoporotic osteoarthritis via affecting TGF-β1 expression in cartilage and the metabolism of subchondral bone. Osteoporotic osteoarthritis was induced by a combination of anterior cruciate transection (ACLT) and ovariectomy (OVX). Rats were divided into five groups (n = 12): The sham group, the ACLT group, the OVX group, the ACLT + OVX group, and the RAL group (ACLT + OVX + RAL, 6.25 mg/kg/day for 12 weeks). Assessment was performed by histomorphology, microcomputed tomography (micro-CT) scan, immunohistochemistry, and tartrate-resistant acid phosphatase (TRAP) staining. We found that severe cartilage degeneration was shown in the ACLT + OVX group. The histomorphological scores, the levels of TGF-β1, and its related catabolic enzymes and osteoclasts numbers in the ACLT + OVX group were higher than those in other groups (p < 0.05). Furthermore, structure model index (SMI) and trabecular spacing (Tb.Sp) were decreased (p < 0.05), while bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular number (Tb.N) were increased by RAL compared with the ACLT + OVX group (p < 0.05). Our findings demonstrated that RAL in clinical doses retards the development of osteoporotic osteoarthritis by inhibiting the overexpression of TGF-β1 in cartilage and regulating the metabolism of subchondral bone. These results provide support for RAL in the expansion of clinical indication for prevention and treatment in postmenopausal osteoarthritis.
Association of Basic Medical Sciences of FBIH
2021-06-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5142
10.17305/bjbms.2020.5142
Biomolecules and Biomedicine; Vol. 21 No. 3 (2021); 284-293
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5142/1926
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5142/2175
Copyright (c) 2020 Shao-Hua Ping, Fa-Ming Tian, Hao Liu , Qi Sun, Li-Tao Shao, Qiang-Qiang Lian, Liu Zhang
oai:ojs2.www.bjbms.org:article/5379
2021-11-21T09:23:22Z
bjbms:PAT
driver
Expression of NEDD9 and connexin-43 in neoplastic and stromal cells of gastric adenocarcinoma
Lerotić, Ivan
Vuković, Petra
Hrabar, Davor
Misir, Zvonimir
Kruljac, Ivan
Pavić, Tajana
Forgač, Jelena
Ćaćić, Petra
Ulamec, Monika
Gastric cancer
NEDD9
conexin-43
microenvironment
metastases
Gastric cancer is related to high mortality rates and advanced disease stage at the time of diagnosis. Its carcinogenesis is extensively studied and is associated with genetic and epigenetic changes, changed the interaction between tumor and adjacent stromal cells, and changes in the microenvironment molecule status. Neural precursor cell-expressed developmentally down-regulated 9 (NEDD9) affects different signaling proteins and pathways, apoptosis, adhesion, cell migration, and invasiveness. Connexin-43 (Cx43) also assists in intercellular communications and has several channel-independent functions. Aberrant expression of those two gap junction proteins plays an essential role in metastatic processes. Our scope was to detect the expression of Cx43 and NEDD9 in epithelial and stromal gastric cancer compartments and its relation to tumor progression and lymph node metastases. Cancer tissue from 53 cases of node-negative and 55 cases of node-positive primary gastric carcinoma patients was analyzed for Cx43 and NEDD9 expression by immunohistochemical assay, and the results were correlated with the remaining clinical and pathological findings and survival. In our cohort of patients with lymph node metastases, we detected higher expression of epithelial Cx43 in the primary tumor and stromal Cx43 expression correlated with both epithelial NEDD9 (rho = 0.453) and stromal NEDD9 (rho = 0.484). Higher epithelial Cx43 and NEDD9 expression were associated with higher mortality (HR 1.54, 95% CI 1.01-2.37, p = 0.048). Epithelial Cx43 expression, both epithelial and stromal NEDD9 expression, T and N status were all independently associated with shorter survival. In summary, our findings suggest that increased expression of both epithelial and stromal NEDD9 and epithelial Cx43 could potentially be used as prognostic gastric cancer biomarkers.
Association of Basic Medical Sciences of FBIH
2021-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5379
10.17305/bjbms.2020.5379
Biomolecules and Biomedicine; Vol. 21 No. 5 (2021); 542-548
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5379/1924
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5379/2361
Copyright (c) 2021 Ivan Lerotić, Petra Vuković, Davor Hrabar, Zvonimir Misir, Ivan Kruljac, Tajana Pavić, Jelena Forgač, Petra Čačić, Monika Ulamec
oai:ojs2.www.bjbms.org:article/5407
2022-06-20T18:47:04Z
bjbms:PAT
driver
Significance of chromogranin A and synaptophysin in medullary thyroid carcinoma
Tomita, Tatsuo
Calcitonin
chromogranin A
immunohistochemistry
medullary thyroid carcinoma
multiple endocrine neoplasm
neuroendocrine tumorsd
synaptophysin
thyroid
Medullary thyroid carcinoma (MTC) is a relatively rare thyroid carcinoma of C-cell deviation and produces and secrete calcitonin (CT) and chromogranin A (CgA) into the blood. Thus, both CT and CgA are immunohistochemical and serum markers for MTCs. MTC occurs in both sporadic and inheritable cases and the hallmark of inheritable cases in multiple endocrine neoplasm 2 (NEN2) is MTC. MEN2 cases represent 30% of MTCs through germline RET protooncogene mutation and occur in younger ages involving bilateral thyroid lobes. Sporadic cases are 70% of cases of solitary tumor and occur in older ages. CgA and synaptophysin (SPY) are the two, most widely used and reliable immunohistochemical markers for neuroendocrine tumors including MTCs. This study aimed to detect different immunohistochemical staining patterns for CgA and SPY between non-symptomatic small, microscopic lesions and invading larger aggressive tumors in both MEA2 cases and sporadic cases. There was different CgA and SPY immunostaining in MEA2 cases where small tumors (≤ 0.3 cm) were lesser immunostained for CgA and SPY, despite strong staining for CT, compared to the larger (≥ 0.5cm) tumors, stronger immunostained for CgA. There was also different CgA and SPY immunohistochemical staining in sporadic cases between small lesion (≤ 0.5 cm) and larger tumors (≥ 1.0cm). One small sporadic tumor (0.5 x 0.3 cm) was strongly and weakly, patchy (about 10% of tumor tissue) stained for CgA and SPY, respectively, while larger sporadic tumors were diffusely, stronger stained for CgA and SPY. Therefore, stronger CgA and SPY immunostaining for larger tumors in both MEA2 and sporadic cases may be used as independent aggressive immunohistochemical markers for MTCs.
Association of Basic Medical Sciences of FBIH
2021-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5407
10.17305/bjbms.2020.5407
Biomolecules and Biomedicine; Vol. 21 No. 5 (2021); 535-541
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5407/2113
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5407/2360
Copyright (c) 2020 Tatsuo Tomita
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/5422
2021-07-19T20:25:20Z
bjbms:PAT
driver
Dishevelled family proteins (DVL1-3) expression in intrauterine growth restriction (IUGR) placentas
Sola, Ida Marija
Serman, Alan
Karin-Kujundzic, Valentina
Paic, Frane
Skrtic, Anita
Slatina, Paula
Kakarigi, Luka
Vranic, Semir
Serman, Ljiljana
intrauterine growth restriction
placenta
Dishevelled family proteins
DVL1
DVL2
DVL3
Dishevelled family proteins (DVL1, DVL2, and DVL3) are cytoplasmic proteins that are involved in canonical and non-canonical Wnt signaling pathway during embryonic development. The role of DVL proteins in the placental tissue remains mostly unknown. In the current study, we explored the role of Dishevelled proteins in naturally invasive tissue, trophoblast. Formalin-fixed paraffin-embedded samples of 15 term placentas from physiologic term pregnancies and 15 term placentas from pregnancies complicated with intrauterine growth restrictions (IUGR) were used for the study. Expression levels of mRNA for DVL1, DVL2, and DVL3 in placentas were analyzed by quantitative real-time PCR (qRTPCR). DVL1, DVL2, and DVL3 protein expression were semi-quantitatively analyzed using immunohistochemistry. The expression of DVL2 and DVL3 proteins was significantly higher in trophoblasts in placental villi from IUGR pregnancies compared with the control group of term placentas. In contrast, DVL3 protein expression was significantly higher in endothelial cells in placental villi from IUGR pregnancies compared with normal term placentas. The observed differences at protein levels between normal and IUGR placentas were not confirmed at the mRNA levels of DVL genes. Our data indicate the active involvement of DVL proteins in IUGR-related placentas. No significant changes were observed in DVL mRNA levels between the two groups of placentas. Further studies are required to explore the clinical relevance of these observations.
Association of Basic Medical Sciences of FBIH
2021-08-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5422
10.17305/bjbms.2020.5422
Biomolecules and Biomedicine; Vol. 21 No. 4 (2021); 447-453
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5422/1976
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5422/2339
Copyright (c) 2020 Ida Marija Sola, Alan Serman, Valentina Karin-Kujundzic, Frane Paic, Anita Skrtic, Paula Slatina, Luka Kakarigi, Semir Vranic, Ljiljana Serman
oai:ojs2.www.bjbms.org:article/5437
2021-11-21T09:24:03Z
bjbms:PAT
driver
The impact of Ki-67 index, squamous differentiation, and several clinicopathologic parameters on the recurrence of low and intermediate-risk endometrial cancer
Ocak, Birol
Atalay, Fatma Öz
Sahin, Ahmet Bilgehan
Ozsen, Mine
Dakiki, Bahar
Türe, Seray
Mesohorli, Merve
Odman , Hikmet Utku
Tanrıverdi, Özgür
Ocakoğlu, Gökhan
Bayrak, Mehmet
Ozan, Hakan
Demiröz, Candan
Sali, Seda
Orhan , Sibel Oyucu
Deligönül, Adem
Cubukcu, Erdem
Evrensel , Türkkan
Low-intermediate risk
endometrioid endometrial carcinoma
recurrence
squamous differentiation
Ki-67
Endometrial endometrioid carcinoma (EEC) represents approximately 75-80% of endometrial carcinoma cases. Three hundred and thirty-six patients with EEC followed-up in the authors’ medical center between 2010 and 2018 were included in our study. Two hundred and seventy-two low and intermediate EEC patients were identified using the European Society for Medical Oncology criteria and confirmed by histopathological examination. Recurrence was reported in 17 of these patients. The study group consisted of patients with relapse. A control group of 51 patients was formed at a ratio of 3:1 according to age, stage, and grade, similar to that in the study group. Of the 17 patients with recurrent disease, 13 patients (76.5%) were Stage 1A, and 4 patients (23.5%) were Stage 1B. No significant difference was found in age, stage, and grade between the case and control groups (p > 0.05). Body mass index, parity, tumor size, lower uterine segment involvement, SqD, and Ki-67 index with p<0.25 in the univariate logistic regression analysis were included in the multivariate analysis. Ki-67 was statistically significant in multivariate analysis (p = 0.018); however, there was no statistical significance in SqD and other parameters. Our data suggest that the Ki-67 index rather than SqD needs to be assessed for recurrence in patients with low- and intermediate-risk EEC.
Association of Basic Medical Sciences of FBIH
2021-02-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5437
10.17305/bjbms.2020.5437
Biomolecules and Biomedicine; Vol. 21 No. 5 (2021); 549-554
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5437/2115
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5437/2362
Copyright (c) 2021 Birol Ocak, Fatma Öz Atalay, Ahmet Bilgehan Sahin, Mine Ozsen, Bahar Dakiki, Seray Türe, Merve Mesohorli, Hikmet Utku Odman , Özgür Tanrıverdi, Gökhan Ocakoğlu, Mehmet Bayrak, Mehmet Bayrak, Hakan Ozan, Candan Demiröz, Seda Sali, Sibel Oyucu Orhan , Adem Deligönül, Erdem Cubukcu, Türkkan Evrensel
oai:ojs2.www.bjbms.org:article/5491
2021-11-19T18:16:04Z
bjbms:PAT
driver
Effects of L-dopa on expression of prolactin and synaptotagmin IV in 17-beta-estradiol-induced prolactinomas of ovariectomized hemiparkinsonian rats
Zorovic, Maja
Kolmančič, Kaja
Živin, Marko
Parkinson's disease
synaptotagmin IV
L-dopa
prolactin
prolactinoma
17-beta-estradiol
Parkinson’s disease (PD) is a long-term degenerative disorder of the central nervous system that mainly affects the motor system. Dopamine precursor levodopa (L-dopa) is used as the first-line treatment for PD. Evidence suggests neuroprotective effects of estrogens in PD. Since both 17b-estradiol (E2) and L-dopa act as regulators of prolactin (PRL) secretion from the pituitary gland, we investigated their effect on the expression of PRL in prolactinomas that developed in ovariectomized hemiparkinsonian rats treated with E2. We also investigated the effect of E2 and L-dopa on the expression of synaptotagmin IV (Syt IV), an immediate early gene whose product is abundant in the pituitary gland and was found to be highly co-expressed with PRL in lactotrophs (>90%). The hemiparkinsonian rat model was obtained by unilateral lesioning of dopaminergic nigrostriatal neurons. Rats received silastic tubing implants with E2 and were treated with L-dopa. Enzyme-linked immunosorbent assay and immunohistochemistry were used to assess the serum concentrations of PRL and E2 and expression of PRL and Syt IV in the tissue of adenohypophysis, respectively. We found that high levels of serum E2 were associated with the upregulation of Syt IV and PRL in PRL-ir cells, while treatment with L-dopa decreased the size of prolactinomas and downregulated Syt IV but had no effect on PRL expression or serum concentrations.
Association of Basic Medical Sciences of FBIH
2021-12-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5491
10.17305/bjbms.2021.5491
Biomolecules and Biomedicine; Vol. 21 No. 6 (2021); 702-711
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5491/2127
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5491/2397
Copyright (c) 2021 Maja Zorovic, Kaja Kolmančič, Marko Živin
oai:ojs2.www.bjbms.org:article/5891
2023-03-20T19:24:35Z
bjbms:PAT
driver
Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
Wang, Zishan
Li, Chenwei
Li, Xinjian
Shi, Jianguang
Wu, Weijie
Genetic polymorphism;
esophageal cancer
VEGF
angiogenesis
rs35569394
chemotherapy
The objective of this study was to investigate the possible association between the single nucleotide polymorphism (SNP), rs35569394, of the vascular endothelial growth factor gene (VEGF) and the risk of esophageal cancer (EC) in the Han Chinese population. A total of 290 EC subjects and 322 ethnically matched unrelated healthy controls free from the esophageal disease were studied. Genomic DNA was isolated from peripheral blood by salting out. Genotyping of VEGF rs35569394 polymorphism was carried out via polymerase chain reaction followed by agarose gel electrophoresis. The results showed that the distribution of genotypes was significantly different across the gender groups (p=0.032) and clinical stages (p=0.034). VEGF rs35569394 was associated with EC risk (p= 0.012, OR=1.34). A gender analysis break-down showed that rs35569394-D allele frequency was significantly higher in females than in the controls (p=0.0004, OR=1.81). Moreover, significant associations were also found in females under the dominant model (II versus ID+DD: χ2=8.18, p=0.003, OR=2.12) and the recessive model (II+ID versus DD: χ2=8.25, p=0.004, OR=2.39). Additionally, we found that the genotype, rs35569394-DD, was associated with a complete response + partial response to chemotherapy when compared with rs35569394-II (χ2=4.67, p=0.030, OR=0.47). In conclusion, our case-control study showed that the VEGF rs35569394 was significantly associated with the clinical stages and the increased risk of EC in Han Chinese females. In addition, the genotype rs35569394-DD showed a better response to chemotherapy.
Association of Basic Medical Sciences of FBIH
2023-03-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5891
10.17305/bjbms.2021.5891
Biomolecules and Biomedicine; Vol. 23 No. 2 (2023); 271–276
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5891/2204
https://www.bjbms.org/ojs/index.php/bjbms/article/view/5891/2732
Copyright (c) 2021 Zishan Wang, Chenwei Li, Xinjian Li, Jianguang Shi, Weijie Wu
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/6110
2022-11-21T10:24:35Z
bjbms:PAT
driver
Poorly differentiated clusters and tumor budding are important prognostic factors in colorectal carcinomas
Jurescu, Aura
Dema, Alis
Văduva, Adrian
Gheju, Adelina
Vița, Octavia
Barna, Robert
Lăzureanu, Codruța
Cornianu, Marioara
Tăban, Sorina
Duță, Ciprian
Pantea, Stelian
Colorectal carcinomas
tumor budding
poorly differentiated clusters
grading system
prognostic factors
The aim of our study was to assess the prognostic value of the two new grading systems based on the quantification of tumor budding - TB (GBd) and poorly differentiated clusters - PDCs (PDCs-G) in colorectal carcinomas (CRC). We performed a retrospective study on 71 CRC patients who underwent surgery at the Emergency County Hospital, Timișoara. CRC cases were classified based on haematoxylin-eosin slides, using the conventional grading system, GBd and PDCs-G, respectively. We used two-tier and three-tier grading schemes for each system. Subsequently, we evaluated associations with other prognostic factors in CRC. Based on the three-tier GBd (GBd-3t) most cases (34/69, 49.27%) were classified as G3Bd-3t, while based on the conventional grading system, the majority of the cases (55/69, 79.71%) were considered G2. On the other hand, based on the three-tier PDCs-G system (PDCs-G-3t), most cases (31/69, 44.93%) were PDCs-G2-3t. We also noted a more significant association of GBd-3t with other prognostic parameters analyzed, as compared to the conventional grading system. Nodal status, tumor stage, and lymphovascular invasion were strongly correlated with GBd-3t (p=0.0001). Furthermore, we noted that PDCs-G-3t correlated more significantly than the conventional grading system with nodal status (p<0.0001), tumor stage (p=0.0003), lymphovascular invasion (p<0.0001), perineural invasion (p=0.005) and the tumor border configuration (p<0.0001). High GBd and PDCs-G grades correlate directly with other negative prognostic factors in CRC.Thus, these new parameters/classification methods could be used as additional tools for risk stratification in patients with CRC.
Association of Basic Medical Sciences of FBIH
2022-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6110
10.17305/bjbms.2021.6110
Biomolecules and Biomedicine; Vol. 22 No. 2 (2022); 164-177
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6110/2374
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6110/2472
Copyright (c) 2021 Aura Jurescu, Adrian Văduva, Adelina Gheju
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/6181
2022-02-19T18:10:39Z
bjbms:PAT
driver
EWSR1 rearrangement in papillary thyroid microcarcinoma is related to classic morphology and the presence of small-cell phenotype
Kovacevic, Bozidar
Caramelo, Ana
Skuletic, Vesna
Cerovic, Snezana
Eloy, Catarina
Papillary thyroid carcinoma
small cells
EWSR1
EWSR-FLI1
carcinoma of the thyroid
Ewing family tumor elements
The EWSR1 rearrangements with unknown genes were detected in a high percentage of classic variants of papillary thyroid carcinoma. The small-cell carcinoma of the thyroid with Ewing family tumor elements (CEFTE) typically presents with EWSR1-FLI1 rearrangement suggesting the possible role of EWSR-FLI1 translocation in the loss of thyroid differentiation and acquisition of a small-cell phenotype. In order to determine the frequency and association of EWSR1 rearrangements, particularly the EWSR1-FLI1 fusion with clinicopathological features of papillary thyroid microcarcinoma (m-PTC) and the presence of small cells, we analyzed a series of 99 m-PTCs using the fluorescence in situ hybridization method. Ninety cases (90.9%) of m-PTC were positive for small cells. This group of m-PTC has shown more often invasive growth, lymphatics invasion, and moderate/extended intratumoral fibrosis. Three cases out of 99 were inconclusive for EWSR1 rearrangement. Eighty-nine (92.7%) and twenty-seven (28.1%) out of 96 m-PTC cases were positive for EWSR1 rearrangement and EWSR1-FLI1 fusion, respectively. m-PTC with classical architectural pattern presented more frequently with EWSR1 rearrangement relative to m-PTC with other patterns (p = 0.005). Other clinicopathological features were not related to the presence of EWSR1 rearrangement or EWSR1-FLI1 fusion. The percentage of small cells present significantly correlated with the percentage of cells positive for EWSR1-FLI1 fusion (p = 0.05) and EWSR1 rearrangement (p <0.001). EWSR1-FLI1 fusion is not rare in m-PTC and it is associated with the acquisition of small-cell phenotype. The EWSR1 gene rearrangement is a frequent event in m-PTC and is related to the classical pattern of m-PTC.
Association of Basic Medical Sciences of FBIH
2022-02-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6181
10.17305/bjbms.2021.6181
Biomolecules and Biomedicine; Vol. 22 No. 1 (2022); 54-63
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6181/2351
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6181/2447
Copyright (c) 2021 Bozidar Kovacevic, Ana Caramelo, Vesna Skuletic, Catarina Eloy, Snezana Cerovic
oai:ojs2.www.bjbms.org:article/6488
2022-04-01T22:52:50Z
bjbms:PAT
driver
Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension
Milutinović, Aleksandra
Zorc-Pleskovič, Ruda
Type 2 diabetes (DM2)
arterial hypertension
ascending aortic aneurysm
inflammatory cells
pro-inflammatory macrophages
anti-inflammatory macrophages
Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes. Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, N=6) versus hypertensive non-diabetic patients (AH group, N=34). For histologic analysis, the sections were stained with hematoxylin-eosin and Movat pentachrome. The immunohistochemical staining was used to analyze the infiltration of pro-inflammatory (CD68) and anti-inflammatory macrophages (CD163), T helper (CD4) and T killer cells (CD8), and B (CD79a) and plasma cells (CD138) in all three layers of aneurysms of both groups. The statistical significance of the differences between groups was evaluated by ANOVA and the Welch test.
In comparison to the AH group, the DM2 group developed less severe infiltration of pro-inflammatory macrophages (P=0.004) and B cells (P=0.025) in the tunica intima, and tunica media (P=0.049, P=0.007, respectively), and fewer plasma cells in the tunica media (P=0.024) and tunica adventitia (P=0.017). We found no significant differences in the number of T helper, T killer cells, and anti-inflammatory macrophages and in the amount of collagen and elastic fibers, ground substance, and smooth muscle cells in all three layers of the vessel wall. Except in tunica adventitia of DM2 group, there were more collagen fibers overall (P=0.025).
Thus, we conclude that the histological structure of the aneurysm in diabetics without hypertension is almost the same as in hypertensive patients without diabetes. Diabetics had significantly less inflammatory infiltration in all three layers of the vessel wall, and more collagen fibers in tunica adventitia.
Association of Basic Medical Sciences of FBIH
2022-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6488
10.17305/bjbms.2021.6488
Biomolecules and Biomedicine; Vol. 22 No. 2 (2022); 178-184
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6488/2380
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6488/2473
Copyright (c) 2021 Aleksandra Milutinović, Ruda Zorc-Pleskovič
oai:ojs2.www.bjbms.org:article/6767
2022-11-21T10:12:34Z
bjbms:PAT
driver
The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients
Ristic Petrovic, Ana
Stokanović, Dragana
Stojnev, Slavica
Potić Floranović, Milena
Krstić, Miljan
Djordjević, Ivana
Skakić, Aleksandar
Janković Veličković, Ljubinka
bladder cancer
clinical outcome
NOTCH pathway
NOTCH3
immunohistochemistry
Disrupted NOTCH activity is a driving event in urothelial bladder cancer (UBC). After activation by hypoxia, the NOTCH3 receptor participates in tumor cell proliferation, acquisition of the epithelial-mesenchymal transition phenotype, and angiogenesis. The aim was to analyze the association of NOTCH3 expression with histopathological and clinical parameters, and to determine its predictive impact on the clinical outcome in UBC patients. The present research included 614 UBC samples incorporated in paraffin tissue microarrays, evaluated by immunohistochemistry for NOTCH3 expression. The accrual period was four years, while the follow-up period was two years. The membranous expression was semi-quantified (0-3), and the mean degree was 1.81±0.94. Criteria for semi-quantification the NOTCH3 expression were the intensity of the staining and the percentage of positive cells. The samples with negative (0) and weak (1) NOTCH3 immunohistochemical (IHC) score were considered negative, while the samples that showed moderate (2) and strong (3) expression were considered positive. Higher degree of positivity was associated with higher risk of cancer-specific mortality (p<0.001). Independent predictors for cancer-specific mortality were NOTCH3 expression and high stage (p<0.001). NOTCH3 expression was not a statistically significant predictor of recurrence-free survival (p=0.816). This study indicated that NOTCH3 is a predictor of poor outcome, suggesting that the NOTCH3 could be potentially reliable IHC marker for selecting the UBC patients that would require more intensive follow-up, especially if they diagnosed in higher stage, with divergent differentiation in pathological report, and without recurrences which would lead them to more frequent medical assessments.
Association of Basic Medical Sciences of FBIH
2022-07-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
text/xml
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6767
10.17305/bjbms.2021.6767
Biomolecules and Biomedicine; Vol. 22 No. 4 (2022); 523-530
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6767/2568
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6767/2429
Copyright (c) 2022 Ana Ristic Petrovic, Dragana Stokanović, Slavica Stojnev, Milena Potić Floranović, Miljan Krstić, Ivana Djordjević, Aleksandar Skakić, Ljubinka Janković Veličković
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/6841
2022-06-01T08:05:54Z
bjbms:PAT
driver
Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp
Tenyi, Ana
Nemeth, Lidija
Golež, Aljaž
Cankar, Ksenija
Milutinović, Aleksandra
Pulse oximetry
electrical sensibility test
histology
volume density of blood vessels and myelinated nerve fibers
In dentistry, indirect diagnostic methods such as electrical sensibility testing and pulse oximetry are used to assess the status of the pulp. Our study aimed to determine the correlation between hemoglobin oxygen saturation and vascular volume density (Vvasc). We also wanted to examine an electrical sensibility test and the volume density of myelinated nerve fibers (Vnerv). Twenty-six intact permanent premolars were included in the study. For histological analysis, the pulp tissue was stained with hematoxylin-eosin and immunohistochemically for von Willebrand factor and S100 to detect blood vessels and myelinated nerve fibers, respectively. The stereological analysis was used to determine the Vvasc and Vnerv. Statistical analysis was done using the Pearson correlation test and Welch’s ANOVA test. Histological analysis showed that the pulp tissue was strongly vascularized and innervated. A significant positive correlation was found between Vvasc and hemoglobin oxygen saturation levels (p=0.030). A significant negative correlation was found between Vnerv and the lowest electrical voltage that patient felt (p=0.033). According to the maturity of the dental apex, teeth were divided into a group with open (N=6, OA group) and closed apex (N=20, CA group). We found that pulps in the CA group had higher Vnerv than the OA group (p=0.037). In contrast, there were no significant differences in Vvasc of the pulp tissue (p=0.059), oxygen saturation (p=0.907), or electrical voltage (p=0.113) between both groups. We can conclude that the measurement of pulse oximetry and electrical sensibility test reflect the morphology of healthy pulp tissue independently of the maturity of the dental apex.
Association of Basic Medical Sciences of FBIH
2022-06-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6841
10.17305/bjbms.2021.6841
Biomolecules and Biomedicine; Vol. 22 No. 3 (2022); 374-381
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6841/2435
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6841/2517
Copyright (c) 2022 Ana Tenyi, Lidija Nemeth, Aljaž Golež, Ksenija Cankar, Aleksandra Milutinović
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/6935
2022-08-21T17:56:07Z
bjbms:PAT
driver
Small cell variant of chromophobe renal cell carcinoma: Clinicopathologic and molecular-genetic analysis of 10 cases.
Rogala, Joanna
Kojima, Fumiyoshi
Alaghehbandan, Reza
Ptakova, Nikola
Bravc, Ana
Bulimbasic, Stela
Perez Montiel, Delia
Slisarenko, Maryna
Ali, Leila
Kuthi, Levente
Pivovarcikova, Kristyna
Michalova, Kvetoslava
Bartovic, Boris
Bartos Vesela, Adriena
Dolejsova, Olga
Michal, Michal
Hes, Ondrej
kidney
chromophobe renal cell carcinoma
small cell variant
The morphologic diversity of chromophobe renal cell carcinoma (ChRCC) is well-known. Aside from typical morphology, pigmented adenomatoid, multicystic and papillary patterns have been described. Ten cases of CHRCC composed of small cell population in various percentages were analysed, using morphologic parameters, immunohistochemistry and next-generation sequencing (NGS) testing. Patients were five males and five females, with age ranging from 40 to 78years. The size of tumors ranged from 2.2 cm to 11 cm (mean 5.17 cm). Small cell component comprised 10 to 80% of the tumor volume, while the remaining was formed by cells with classic ChRCC morphology. The immunohistochemical profile of the small cell component was consistent with typical ChRCC immunophenotype, with CD117 and CK7 positivity. Neuroendocrine markers were negative. Mutations of 13 genes were found: DCIER1, FGFR3, JAK3, SUFO, FAM46C, FANCG, MET, PLCG2, APC, POLE, EPICAM, MUTYH and AR. However, only the PLCG2 mutation is considered pathogenic.The small cell variant of ChRCC further highlights and expand upon existing morphologic heterogeneity spectrum. Recognition of small cell variant of CHRCC is not problematic in tumors, where the “classic” CHRCC component is present. However, in limited material (i.e., core biopsy), this may present a diagnostic challenge. Based on the limited follow-up data available, it appears that the small cell tumor component had no impact on prognosis, since there was no aggressive behavior documented. Awareness of this unusual pattern and applying additional sections to find classic morphology of ChRCC, as well as excluding neuroendocrine nature by immunohistochemistry, may help resolve difficult cases.
Association of Basic Medical Sciences of FBIH
2022-07-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
text/xml
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6935
10.17305/bjbms.2021.6935
Biomolecules and Biomedicine; Vol. 22 No. 4 (2022); 531-539
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6935/2569
https://www.bjbms.org/ojs/index.php/bjbms/article/view/6935/2465
Copyright (c) 2022 Joanna Rogala, Fumiyoshi Kojima, Reza Alaghehbandan, Nikola Ptakova, Ana Bravc, Stela Bulimbasic, Delia Perez Montiel, Maryna Slisarenko, Leila Ali, Levente Kuthi, Kristyna Pivovarcikova, Kvetoslava Michalova, Boris Bartovic, Adriena Bartos Vesela, Olga Dolejsova, Michal Michal, Ondrej Hes
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/7567
2023-02-03T14:39:36Z
bjbms:PAT
driver
Clinical and histopathological characteristics of COL4A3 c.2881+1G>A variant causing Alport spectrum disorders in Croatian population
Horaček, Matija
Nikuševa Martić, Tamara
Šenjug, Petar
Šenjug Perica, Marija
Oroz, Maja
Kuzmac, Sania
Klarić, Dragan
Glavina Durdov, Merica
Saraga, Marijan
Milošević, Danko
Batinić, Danica
Ćorić, Marijana
Paić, Frane
Galešić Ljubanović, Danica
Alport syndrome
thin basement membrane nephropathy
proteinuria
collagen type IV
α3 chain of collagen IV
COL4A3 c.2881 1G>A variant
targeted next-generation sequencing
Alport syndrome (AS) and thin basement membrane nephropathy (TBMN) are part of the spectrum of kidney disorders caused by pathogenic variants in α3, α4, or α5 chains of the collagen type IV, the major structural component of the glomerular basement membrane (GBM). Using targeted next-generation sequencing (NGS), 34 AS/TBMN patients (58.8% male) from 12 unrelated families were found positive for heterozygous c.2881+1G>A variant of the COL4A3gene, that is considered disease-causing. All patients were from the continental or island part of Croatia. Clinical, laboratory, and histopathological data collected from the medical records were analyzed and compared to understand the clinical course and prognosis of the affected patients. At the time of biopsy or first clinical evaluation, the mean age was 31 years (median: 35 years; range: 1 – 72 years). Hematuria was present in 33 patients (97.1%) and 19 (55.9%) patients had proteinuria. There were 6 (17.6%) patients with hearing loss, 4 (11.8%) with ocular lesions, and 11 (32.4%) with hypertension. Twenty-three (67.6%) patients had proteinuria at follow-up, and 5 (14.7%) patients with the median age of 48 years (range: 27-55) progressed to kidney failure, started dialysis, or underwent kidney transplantation. Of the 13 patients who underwent kidney biopsy, 4 (30.8%) developed focal segmental glomerulosclerosis (FSGS), and 8 (66.7%) showed lamellation of the GBM, including all patients with FSGS. It is essential to conduct a detailed analysis of each collagen type IV genetic variant to optimize the prognosis and therapeutic approach for affected patients.
Association of Basic Medical Sciences of FBIH
2023-01-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
text/xml
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7567
10.17305/bjbms.2022.7567
Biomolecules and Biomedicine; Vol. 23 No. 1 (2023); 89-100
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7567/2693
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7567/2557
Copyright (c) 2022 Matija Horaček, Tamara Nikuševa Martić, Petar Šenjug, Marija Šenjug Perica, Maja Oroz, Sania Kuzmac, Dragan Klarić, Merica Glavina Durdov, Marijan Saraga, Danko Milošević, Danica Batinić, Marijana Ćorić, Frane Paić, Danica Galešić Ljubanović
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/7574
2022-11-21T09:57:18Z
bjbms:PAT
driver
Relationship between PD-L1 expression and prognostic factors in high-risk cutaneous squamous and basal cell carcinoma
Yülek, Özden
Batur, Şebnem
Özcan, Kerem
Yol, Cansu
Aydın Ülgen, Övgü
Cutaneous squamous cell carcinoma
basal cell carcinoma
PD-L1
This study aimed to investigate the programmed cell death-ligand 1 (PD-L1) expression in cutaneous squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC) and its relationship with prognostic factors in tumors that are not in the head and neck region and are therefore relatively less exposed to the sun. This retrospective cross-sectional study included 25 invasive cSCC and 42 BCC cases with a diameter ≥ 2 cm located outside the head and neck region from 2010 to 2018. The biopsy samples were examined based on the membranous PD-L1 (22C3 clone) staining. Staining results were scored as follows: 0, no staining (negative); 1, < 10% PD-L1 positivity of tumor cells; and 2, ≥ 10% PD-L1 positivity of tumor cells. PD-L1 positivity was not seen in any BCC cases, whereas 11 (44%) of cSCC cases were PD-L1 positive. No significant relationship was observed between PD-L1 expression and prognostic parameters, including tumor diameter, tumor depth, and lymphovascular or perineural invasion in the cSCC group. PD-L1 expression was not associated with prognostic factors in the early stages of BCC and SCC located outside the head and neck region. Therefore, investigating the PD-L1 expression seems to be more relevant in patients with advanced-stage disease.
Association of Basic Medical Sciences of FBIH
2022-10-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
text/xml
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7574
10.17305/bjbms.2022.7574
Biomolecules and Biomedicine; Vol. 22 No. 6 (2022); 894-900
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7574/2632
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7574/2552
Copyright (c) 2022 Özden Yülek, Şebnem Batur, Kerem Özcan, Cansu Yol, Övgü Aydın Ülgen
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/7676
2023-02-03T14:35:11Z
bjbms:PAT
driver
Acquired atresia of the external auditory canal and canaloplasty with Thiersch graft reconstruction: Outcomes and complications
Pace, Annalisa
Rossetti, Valeria
Iannella, Giannicola
Visconti, Irene Claudia
Milani, Alessandro
Polimeni, Roberta
Maniaci, Antonino
Cocuzza, Salvatore
Re, Massimo
Magliulo, Giuseppe
acquired atresia
external auditory canal
endoaural surgery
canaloplasty
skin graft
Acquired atresia of the external auditory canal (EAC) is a rare disease characterized by otorrhea and progressive hearing loss. Clinically, it is differentiated into two stages: the wet stage and the dry stage. The dry stage does not respond to pharmacological treatment and has to be treated surgically. One surgical option is canaloplasty of the EAC with Thiersch graft reconstruction. This study aimed to report the follow-up outcomes (otomicroscopic signs and pure tone audiometry [PTA]) in patients with acquired atresia treated with this technique. Eighteen adult patients surgically treated for acquired atresia of the EAC between 2010 and 2020 were enrolled. All underwent canaloplasty with Thiersch graft reconstruction by one senior surgeon. Otomicroscopy and PTA results were evaluated before and after surgery. Postsurgical follow-up was performed at 1-3-6-12 months and then annually. Presurgical otomicroscopic examination revealed stenosis that occluded more than 75% of the EAC in all patients, and preoperative PTA showed conductive hearing loss in 89% of patients. However, postsurgical otomicroscopic examination showed that 94% of patients had a normal EAC diameter after one year, and only one patient had anterior blunting and recurrent atresia. In addition, postsurgical PTA evidenced a normal range in 89% of patients after one year. In conclusion, acquired atresia of the EAC is a troublesome disease usually associated with hearing loss. Therefore, treatment is chosen to resolve its symptoms. The results demonstrate evidence that canaloplasty with Thirsch graft may be a suitable surgical method considering the lower incidence of recurrence and the excellent hearing outcomes.
Association of Basic Medical Sciences of FBIH
2023-01-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7676
10.17305/bjbms.2022.7676
Biomolecules and Biomedicine; Vol. 23 No. 1 (2023); 73-76
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7676/2625
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7676/2691
Copyright (c) 2022 Annalisa Pace, Valeria Rossetti, Giannicola Iannella, Irene Claudia Visconti, Alessandro Milani, Roberta Polimeni, Antonino Maniaci, Salvatore Cocuzza, Massimo Re, Giuseppe Magliulo
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/7677
2023-02-03T14:37:04Z
bjbms:PAT
driver
Autophagy attenuates osteoarthritis in mice by inhibiting chondrocyte pyroptosis and improving subchondral bone remodeling
Yan, Jiangbo
Feng, Gangning
Yang, Yong
Ding, Dong
Ma, Long
Zhao, Xin
Chen, Xiaolei
Wang, Hui
Chen, Zhirong
Jin, Qunhua
Osteoarthritis
chondrocyte
autophagy
pyroptosis
subchondral bone
Osteoarthritis (OA) is an age-related degenerative disease characterized by cartilage degeneration and abnormal bone remodeling in the subchondral bone. Autophagy maintains cellular homeostasis by self-phagocytosis. However, the underlying mechanisms of autophagy on the pathological progression of OA are still unknown. This study assessed the effects of autophagy on cartilage and subchondral bone in a mouse OA model. A mouse OA model was induced using destabilization of the medial meniscus (DMM) surgery. Assessment was performed by histomorphology, microcomputed tomography (micro-CT), immunohistochemical, immunofluorescent, and tartrate-resistant acid phosphatase (TRAP) staining. Our data revealed that autophagy can significantly delay the pathological progression of OA by increasing the thickness of hyaline cartilage and decreasing the thickness of calcified cartilage, increasing the subchondral bone volume fraction and bone mineralization density, and decreasing trabecular separation in the early stages of OA (2 weeks), whereas the opposite is true in the late stages of OA (8 weeks). Mechanistically, activation of autophagy in cartilage increased the expression of type II collagen (Col II), decreased the expression of matrix metalloproteinase 13 (MMP 13) and decreased the pyroptosis mediated by NOD-like receptor protein 3 (NLRP3) inflammasome by decreasing the expression of NLRP3, caspase-1, gasdermin D (GSDMD), and IL-1β. In the subchondral bone, activation of autophagy decreased the generation of mature osteoclasts at the early stages of OA (2 weeks) mainly by reducing the receptor activator for nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) ratio, while it decreased osteoblastogenesis by reducing Runt-related transcription factor 2 (Runx2) expression significantly in the late stages of OA (8 weeks). In conclusion, autophagy may delay the pathological progression of OA in mice by inhibiting chondrocyte pyroptosis and improving subchondral bone remodeling.
Association of Basic Medical Sciences of FBIH
2023-01-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
text/xml
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7677
10.17305/bjbms.2022.7677
Biomolecules and Biomedicine; Vol. 23 No. 1 (2023); 77-88
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7677/2692
https://www.bjbms.org/ojs/index.php/bjbms/article/view/7677/2559
Copyright (c) 2022 Jiangbo Yan, Gangning Feng, Yong Yang, Dong Ding, Long Ma, Xin Zhao, Xiaolei Chen, Hui Wang, Zhirong Chen, Qunhua Jin
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/8034
2023-03-20T19:23:47Z
bjbms:PAT
driver
Deletion of sphingosine kinase 2 attenuates cigarette smoke-mediated chronic obstructive pulmonary disease-like symptoms by reducing lung inflammation
Chen, Yanhui
Zhang, Yongrong
Rao, Cheng
Huang, Jieyun
Qing, Qiong
SphK2
COPD
CFTR
S1P
pulmonary inlammation
Cigarette smoke (CS) is the leading cause of chronic obstructive pulmonary disease (COPD), which is characterized by chronic bronchial inflammation and emphysema. Growing evidence supports the hypothesis that dysfunctional cystic fibrosis transmembrane conductance regulator (CFTR) is critically involved in the pathogenesis of CS-mediated COPD. However, the underlying mechanism remains unclear. Here, we report that supressed CFTR expression is strongly associated with abnormal phospholipid metabolism and increased pulmonary inflammation. In a CS-exposed mouse model with COPD-like symptoms, we found that pulmonary expression of sphingosine kinase 2 (SphK2) and sphingosine-1-phosphate (S1P) secretion were significantly upregulated. Therefore, we constructed a SphK2 gene knockout (SphK2-/-) mouse. After CS exposure for six months, histological lung section staining showed disorganized alveolar structure, increased pulmonary fibrosis, and emphysema-like symptoms in wild-type (WT) mice, which were less pronounced in SphK2-/- mice. Further, SphK2 deficiency also decreased CS-induced pulmonary inflammation, which was reflected by a remarkable reduction in pulmonary infiltration of CD45+CD11b+ neutrophils subpopulation and low levels of IL-6 and IL-33 in bronchial alveolar lavage fluid. However, treatment with S1P receptor agonist suppressed CFTR expression and increased Nf-κB-p65 expression and its nuclear translocation in CS-exposed SphK2-/-mice, which also aggravated small airways fibrosis and pulmonary inflammation. In contrast, inhibition of S1P signaling with the S1P receptor analogue FTY720 rescued CFTR expression, suppressed Nf-κB-p65 expression and nuclear translocation, and alleviated pulmonary fibrosis and inflammation after CS exposure. Our results demonstrate that SphK2-mediated S1P production plays a crucial role in the pathogenesis of CS-induced COPD-like disease by impairing CFTR activity and promoting pulmonary inflammation and fibrosis.
Association of Basic Medical Sciences of FBIH
2023-03-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/8034
10.17305/bjbms.2022.8034
Biomolecules and Biomedicine; Vol. 23 No. 2 (2023); 259–270
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/8034/2624
https://www.bjbms.org/ojs/index.php/bjbms/article/view/8034/2731
Copyright (c) 2022 Yanhui Chen, Yongrong Zhang, Cheng Rao, Jieyun Huang, Qiong Qing
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/8565
2023-07-03T13:53:51Z
bjbms:PAT
driver
Differences between inflammatory cells infiltrated into tunica intima, media, and adventitia of ascending aortic aneurysms within diabetic and hypertensive patients
Milutinović, Aleksandra
Zivin, Marko
Zorc-Pleskovič, Ruda
type 2 diabetes (DM)
arterial hypertension (AH)
ascending aortic aneurysm
intima
media
adventitia
inflammation
The risk factors that are the most significant for the development of most cardiovascular diseases are arterial hypertension (AH), type 2 diabetes (DM), and inflammation. However, for the development of aortic aneurysms, DM is not one of them. Our study aimed to evaluate the difference between inflammatory infiltration in three individual layers of the ascending aortic aneurysm within diabetic and hypertensive patients. Forty-five patients aged 36 to 80 were divided into a group with diabetic patients without AH (group DM, N=8) and hypertensive patients without DM (group AH, N=37). For the histological analysis, aortic aneurysms were stained with hematoxylin eosin and Movat. We used immunochemical methods to detect pro- (M1), anti-inflammatory (M2) macrophages, T-helper, T-killer cells, B cells, and plasma cells. Statistical analysis was done by independent-samples Kruskal-Wallis test adjusted by Bonferroni correction for multiple tests (P<0.05). We found no difference in the volume density of collagen, elastin, vascular smooth muscle cells (VSMC), and ground substance between groups. In the DM group, there were significantly fewer M2, T-helpers, and T-killers in the media than in the intima and the adventitia (P<0.05). There were no significant differences in the number of M1, B, and plasma cells between all three vascular layers (P<0.05). In the AH group, there were significantly fewer B and plasma cells, T-helper, T-killer cells, M1, and M2 in the media than in the intima and adventitia (P<0.05). Our results conclude that the tunica media in the aneurismal wall of the AH group retained immune privilege. In contrast, in the DM group, all three layers were immune-privileged.
Association of Basic Medical Sciences of FBIH
2023-07-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
text/xml
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/8565
10.17305/bb.2022.8565
Biomolecules and Biomedicine; Vol. 23 No. 4 (2023); 596–604
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/8565/2830
https://www.bjbms.org/ojs/index.php/bjbms/article/view/8565/2678
Copyright (c) 2023 Aleksandra Milutinović, Ruda Zorc-Pleskovič
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/9242
2023-11-03T11:20:02Z
bjbms:PAT
driver
Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6 and p16
Terlević, Robert
Ulamec, Monika
Štimac, Goran
Murgić, Jure
Krušlin, Božo
Urothelial carcinoma
bladder cancer
immunohistochemistry (IHC)
molecular subtype
survival analysis
The choice of therapy for muscle-invasive bladder cancer (MIBC) could be influenced by the tumor’s molecular subtype. Currently, well-defined consensus subtypes are based on tumor microarray mRNA data. Clearly defined and easy-to-use surrogate molecular subtypes, based on immunohistochemistry (IHC) performed on whole slides, are needed to make subtyping cost-effective and useful in routine work and future research. To aid in the development of a simple immunohistochemical classifier, a retrospective single-center series of 92 cases of localized bladder cancer was identified. Routine IHC for GATA3, cytokeratins 5 and 6 (CK5/6), and p16 was performed on whole tissue blocks containing muscle-invasive disease. Electronic medical records were retrieved and searched for clinical variables, treatment, and survival data. The mean age was 69.6 years, and 73% were males. Conservative treatment was used in 55% of cases, while cystectomy with chemotherapy was used in 45%. GATA3 and CK5/6 expression divided cases into broad luminal and basal subtypes, respectively, while p16 expression was used to subclassify luminal cases into luminal papillary and luminal unstable types according to the consensus molecular classification. When subtyped in this way, GATA3 and CK5/6 negative cases showed worse overall survival. Molecular subtyping of MIBC on whole slides containing muscle-invasive tumor using only three commonly used, consensus-based antibodies, is a feasible and cost-effective method for detecting subtypes of invasive bladder cancer. Future work combining morphological analysis and IHC is needed to fully translate the consensus molecular classification into a comprehensive, cost-effective subtyping strategy.
Association of Basic Medical Sciences of FBIH
2023-11-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
text/xml
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9242
10.17305/bb.2023.9242
Biomolecules and Biomedicine; Vol. 23 No. 6 (2023); 968–975
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9242/2817
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9242/2971
Copyright (c) 2023 Robert Terlević, Monika Ulamec, Goran Štimac, Jure Murgić, Božo Krušlin
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/9868
2024-03-11T13:32:22Z
bjbms:PAT
driver
The assessment of tumor-infiltrating lymphocytes in invasive apocrine carcinoma of the breast in relation to the HER2 status
Gatalica, Zoran
Kuzmova, Nataliya
Rose, Inga
Ulamec, Monika
Peric-Balja, Melita
Skenderi, Faruk
Vranic, Semir
Breast cancer
special types
apocrine carcinoma
tumor-infiltrating lymphocytes (TILs)
HER2-low
In the current study, we assessed the prevalence and molecular features of HER2-low phenotype in the apocrine carcinomas of the breast (ApoCa) and its relationship with tumor-infiltrating lymphocytes (TILs). A cohort of 64 well-characterized therapy-naïve ApoCa was used. The TIL distribution was assessed using the hematoxylin and eosin whole slide/scanned images following the international TILs working group recommendations. Next-generation sequencing (NGS) was performed in a subset of HER2-low ApoCa. All patients were women, with a mean age of 62 years. Forty-three carcinomas were pure apocrine carcinoma (PAC; ER−/AR+), and the remaining 21 were classified as apocrine-like carcinomas (ALCs; ER+/−, AR+/−). HER2/neu was positive (score 3+ by IHC and/or amplified by FISH) in 20/43 (47%) PAC and 4/21 (19%) ALC. The prevalence of HER2-low expression (scores 1+ or 2+ without HER2 amplification) in ApoCa was 39% without significant differences between PAC and ALC (P = 0.14); however, the HER2-low phenotype was more prevalent in triple-negative PAC than in ALC (P < 0.001). Levels of TILs were low (≤10%) in 74% of ApoCa (median 5%, range 0%–50%). TIL levels were significantly higher in ALC than in PAC (P = 0.02). HER2 status had no impact on TIL distribution (P = 0.45). The genomic profile of HER2-low ApoCa was similar to other subtypes of ApoCa. ApoCa has predominantly low TIL, particularly PAC. The prevalence of the HER2-low phenotype in ApoCa is high, which should have therapeutic and clinical implications given the recently approved therapies with antibody–drug conjugates (ADCs) for HER2-low breast cancers.
Association of Basic Medical Sciences of FBIH
2024-03-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9868
10.17305/bb.2023.9868
Biomolecules and Biomedicine; Vol. 24 No. 2 (2024); 256–261
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9868/2957
Copyright (c) 2023 Zoran Gatalica, Nataliya Kuzumova, Inga Rose, Monika Ulamec, Melita Peric Balja, Faruk Skenderi, Semir Vranić
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/9937
2024-03-11T10:10:55Z
bjbms:PAT
driver
The role of the NY-ESO-1 in the prognosis of gastric cancer
Misir, Zvonimir
Glavčić, Goran
Janković, Suzana
Kruljac, Ivan
Čugura Filipović, Jakša
Čimić, Kristina
Ulamec, Monika
Gastric cancer (GC)
survival
New York esophageal squamous cell carcinoma-1 (NY-ESO-1)
metastases
Gastric cancer (GC) is one of the most common malignancies worldwide and the fourth leading cause of cancer-related deaths. GC is a multifactorial disease influenced by both environmental and genetic factors. Its most critical features include invasiveness and high metastatic potential.Metastasis is a complex process, and our understanding of themechanisms involved remains incomplete. Growing evidence suggests that cancer-testis antigens (CTAs) play a crucial role in the metastatic potential of various tumors. Several studies have linked CTA expression with lower tumor differentiation, higher metastatic potential, and poor chemotherapy response. New York esophageal squamous cell carcinoma-1 (NY-ESO-1) antigen, part of the CTA group, is expressed in tumor tissues, while its expression in normal tissues is restricted to spermatogonia. This study aimed to determine the expression of NY-ESO-1 in primary adenocarcinoma of the stomach, both with and without metastasis in regional lymph nodes, and to compare it with TNM stage, age, gender, and survival. We analyzed GC tissue from 53 node-negative and 55 node-positive primary gastric carcinoma patients for NY-ESO-1 expression using immunohistochemical assay. The results were correlated with clinicopathological parameters and survival. Patients with positive NY-ESO-1 expression in primary tumors had a median survival of 19.0 months (range 14.1–24.0), in contrast to those with negative expression, who had a median survival of 52.0 months (range 0.0–133.3) (chi-square 7.99, P=0.005). T status, N status, and NY-ESO-1 expression were all independently associated with shorter survival. No significant difference in NY-ESO-1 expression in primary tumors was observed concerning lymph node metastasis status. In summary, our findings suggest that increased expression of NY-ESO-1 could potentially serve as a prognostic biomarker for GC.
Association of Basic Medical Sciences of FBIH
2023-12-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9937
10.17305/bb.2023.9937
Biomolecules and Biomedicine; Advanced online
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9937/3127
Copyright (c) 2023 Zvonimir Misir, Goran Glavčić, Suzana Janković, Ivan Kruljac, Jakša Čugura Filipović, Kristina Čimić, Monika Ulamec
https://creativecommons.org/licenses/by/4.0
oai:ojs2.www.bjbms.org:article/9988
2024-01-23T23:10:19Z
bjbms:PAT
driver
Expression of CD31, CD34, and smooth muscle actin (SMA) in endothelial cells of dental pulp vessels
Tenyi, Ana
Milutinović, Aleksandra
Nemeth, Lidija
Dental pulp
endothelial cells
cluster of differentiation 31 (CD31)
smooth muscle actin (SMA)
cluster of differentiation 34 (CD34)
The dental pulp is a highly vascularized and innervated loose connective tissue surrounded by hard dental tissues – enamel and dentine. With the primary dentin formation and the closure of the root apex, the conditions in the dental pulp change and pulp tissue compliance are reduced. Endothelial cells of pulpal blood vessels are highly differentiated and are capable of adaptation to changes in the environment. We aimed to evaluate the phenotypic plasticity of endothelial cells of pulpal blood vessels in permanent premolars with open (N = 6) or closed root apex (N = 30). The pulp tissue was stained with hematoxylin-eosin (HE) for the histological analysis, and immunohistochemically for a cluster of differentiation 31 (CD31), a cluster of differentiation 34 (CD34), and for smooth muscle actin (SMA) to detect vessels with CD31, CD34, and SMA positive endothelial cells. We used the student’s t-test and Pearson correlation test for the statistical analysis. We found a significantly higher percentage of the vessels with CD31 (P = 0.005) and CD34 (P = 0.000) positive endothelial cells in the group of teeth with closed root apex compared to the group with open apex. A significant positive correlation between SMA positive and CD31 positive vessels (P = 0.003) and between CD31 positive and CD34 positive vessels (P = 0.031) was also found. We conclude that the endothelial cells of dental pulp vessels express a small amount of CD31, but have a pronounced expression of SMA and CD34, which indicates their progenitor potential and contractile ability.
Association of Basic Medical Sciences of FBIH
2023-12-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9988
10.17305/bb.2023.9988
Biomolecules and Biomedicine; Advanced online
2831-090X
2831-0896
eng
https://www.bjbms.org/ojs/index.php/bjbms/article/view/9988/3129
Copyright (c) 2023 Ana Tenyi, Aleksandra Milutinović, Lidija Nemeth
https://creativecommons.org/licenses/by/4.0