Eff ects of single-dose neuropeptide Y on levels of hippocampal BDNF , MDA , GSH , and NO in a rat model of pentylenetetrazole-induced epileptic seizure

Epilepsy is one of the most common neurological disorders, characterized by recurrent seizures, which may increase the content of reactive oxygen and nitrogen species. Th e objective of this study was to investigate the eff ects of Neuropeptide Y on oxidative and nitrosative balance and brain-derived neurotrophic factor levels induced by pentylenetetrazole (a standard convulsant drug) in the hippocampus of Wistar rats. Th ree groups of seven rats were treated intraperitoneally as follows: group  (saline + saline)  ml saline, group  (salin + Pentylenetetrazole)  ml saline  min before Pentylenetetrazole; and group  (Neuropeptide Y + Pentylenetetrazole)  μg/kg Neuropeptide Y  min before  mg/kg Pentylenetetrazole. After  h, the animals were euthanized by decapitation. Hippocampus were isolated to evaluate the malondialdehyde, glutathione, nitric oxide, and brain-derived neurotrophic factor levels in three rat groups. Th e results of this study demonstrated that while intraperitoneally administered neuropeptide Y did not result in a statistically signifi cant diff erence in BDNF levels, its administration caused a statistically signifi cant decrease in malondialdehyde and nitric oxide levels and an increase in glutathione levels in rats with pentylenetetrazole-induced epileptic seizure. Neuropeptide Y were able to reduce nitroxidative damage induced by pentylenetetrazole in the hippocampus of Wistar rats. ©  Association of Basic Medical Sciences of FB&H. All rights reserved


INTRODUCTION
Generalized epilepsy is a chronic disorder characterized by recurrent seizures, which has been shown to cause an increase in reactive oxygen species (ROS) in the brain and thus increased oxidative stress [].Oxidative stress and mitochondrial dysfunction can occur as a consequence of prolonged epileptic seizure and may contribute to seizureinduced brain damage [].Nitric oxide (NO) free radical is a molecular messenger involved in numerous physiological processes.However, several studies on the complex role of NO in the nervous system have demonstrated that NO plays a role in the pathophysiology of brain damage in brain ischemia, trauma, and chronic epilepsy and contributes to neurotoxicity and neural damage associated with several neurodegenerative diseases [, ].Brain-derived neurotrophic factor (BDNF) is a small dimeric protein that belongs to the neurotrophin family and was originally identifi ed as a crucial neuronal survival factor []. BDNF is abundant in the brain.In addition to its ability to promote neuronal proliferation and diff erentiation, BDNF infl uences the shape and number of dendritic spines and thus aff ects the morphological development of neurons [-].Neuropeptide Y (NPY) is widely expressed in the central nervous system where it is involved in various functions including the regulation of circadian rhythms, blood pressure, feeding behaviour, anxiety, memory processing, and cognition [].In the hippocampus, NPY is specifi cally expressed in a subset of GABAergic neurons in the pyramidal cell layer and in the dentate hilus where it is probably released by the activation of intrinsic and projection pathways [].Th ere is also increasing evidence that the peptide exerts powerful anticonvulsant activities and a related neuroprotective eff ect on cornu ammonis (CA) pyramidal neurons [, , ].For these reasons, we studied the acute eff ects of exogenous intraperitoneally (ip) administered NPY in adult rats following pentylenetetrazole (PTZ) intoxication, particularly during the early phases that are reasonably crucial in the establishment of brain injury.Changes in the  [, ].A convulsive dose of  mg/kg PTZ was used for injection, and the rats were housed in Plexiglas cages ( cm ×  cm ×  cm).Following injections, rats were monitored for seizure activity for  min.Animals were sacrifi ced  h after inducing seizures or administering saline.Brains were immediately removed and the hippocampus was dissected.To prepare a  (w/v) homogenate, the hippocampus was weighed and homogenized in cold Tris-HCl buff er (pH .).Next, BDNF, MDA, NO, and GSH levels were measured.Lipid peroxide levels, expressed in terms of MDA, were determined according to the method of Buege and Aust [].GSH was measured according to the method of Ellman [].Nitrite and nitrate were estimated as an index of NO production [].Th e method for determining nitrite and nitrate levels was based on the Griess reagent, which consists of sulfanilamide and N-(naphthyl) ethylenediamine.Protein concentrations of hippocampal homogenates were determined by the method of Lowry et al. [].Th e hippocampal BDNF levels were measured using an ELISA kit (BOSTER ELISA; Boster Bıologıcal Technology., Ltd., CA, USA) according to the manufacturer's instructions.All measurements were performed in duplicate.

Statistical analysis
Statistical analysis was performed using the SPSS sta-tistical package (SPSS Inc., Chicago, IL, USA) for Windows (version .).The values were expressed as the mean ± standard deviation (SD).The significance of differences among the three groups was assessed using oneway analysis of variance (ANOVA) with the post-hoc Tukey test.p < . was considered statistically signifi cant.

RESULTS
Th e mean MDA levels in the hippocampus were signifi cantly higher in the

DISCUSSION
The PTZ-induced clonic seizure paradigm represents an animal model of myoclonic seizures and is very sensitive to changes in seizure susceptibility [].Disinhibition of the inhibitory neurotransmitter GABA through specific interactions with the GABA-gated chloride ionophores and/ or activation of N-methyl-D-aspartate (NMDA) receptors appears to be among the factors involved in the initiation and generalization of PTZ-induced seizures [, ].ROS/reactive nitrogen species (RNS) have been implicated in the pathogenesis of various neurological disorders including epilepsy [].Excitotoxicity and disrupted energy metabolism act in a synergistic manner, leading to nerve cell death in neurodegenerative disorders [].Th ese coop-erative pathways trigger oxidative stress by free radical formation [], and ROS/RNS cause lipid peroxidation with high levels of MDA, resulting in damage to biological membranes [].Epileptic activity causes excessive production of ROS/RNS, a factor believed to be involved in the mechanisms leading to neurodegeneration and cell death [, ].
In the present study, we found that the mean hippocampal MDA levels in rats treated with PTZ to induce epileptic seizures were significantly higher than the control group (SF + SF) and the NPY + PTZ groups.In contrast, statistical analysis showed that PTZ-treated rats had significantly lower GSH levels than saline-and NPY-treated rats.The increased levels of MDA and reduced concentrations of GSH observed in the SF + PTZ group indicated the presence of oxidative stress in epileptic seizure.In the group treated with NPY, there was a statistically significant decrease or increase in MDA or GSH levels, respectively.These findings demonstrate that in addition to its anticonvulsive effects on pre-synaptic Y receptors, NPY contributes to the oxidant-antioxidant balance [, , ].
In the present study, statistically significant levels of NO were detected in the SF + PTZ group, whereas NO levels decreased signifi cantly in the NPY-treated group.NO produced from increased action of the excitatory neurotransmitter glutamate is postulated to be involved in pathophysiology of many models of epilepsy [, , ].In neurons, NO synthesis is stimulated by Ca + infl ux, which is induced by activation of glutamate receptors, preferentially by the NMDA receptors [, ].Bal-Price et al. [] stated that neurons in culture were sensitive to cell death induced by NO, NO donors, or NO-producing cells.A major cause of this sensitivity is inhibition of mitochondrial function by NO, glutamate release, and subsequent excitotoxic death of neurons [, ].High levels of NO lead, in general, to a mixture of nitrosative, nitrative, and oxidative stress, which is referred to as nitroxidative stress [].Th e actions of NO are generally dependent on the following factors: (a) the concentration of NO, (b) the time course of exposure to NO, (c) the presence or absence of a particular ROS at eff ective levels, and (d) the presence or absence of particular pathways in particular cells.Th is indicates that NO is cytoprotective at low levels and toxic at high levels [, , ].NO can also be harmful under oxidative stress and conditions caused by the oxidation and nitrotyrosination of functional proteins [].In our present study, in addition to an increase observed in NO levels, we detected an increase in oxidation levels (high MDA level and low GSH level) in the group with PTZ-induced epilepsy.Th e ability to control ROS is thus critical in neurodegenerative diseases because neuronal damage occurs when the "oxidant-antioxidant" balance is disturbed in favour of excess oxidative stress [, ].In our present study, the bal-  ance observed between oxidative and nitrosative metabolism in the NPY-treated group indicated that exogenous administration of NPY may exert neuroprotective eff ects in epilepsy.
The discovery that limbic seizures increase the levels of mRNAs encoding nerve growth factor [] led to the idea that seizure-induced expression of neurotrophic factors may contribute to the maintenance of structural and functional changes underlying epileptogenesis [-].Recent in vitro and in vivo fi ndings place BDNF in the cascade of electrophysiologic and behavioral changes that underlie the epileptic state [].Transgenic mice overexpressing BDNF exhibit hyperexcitability in the hippocampal CA area and entorhinal cortex and show increased status epilepticus severity in response to systemic injection of kainic acid [].
Accordingly, chronic infusion of BDNF in rat hippocampus eventually results in motor seizures and the infused animals show increased sensitivity to induction of seizures by pilocarpine [].Th e long-term eff ects of BDNF on epileptogenesis were merely studied in a kindling model.Amygdala kindling was impaired in BDNF knockout mice [, ] or by intraventricular infusion of "TrkB receptor bodies" [], and was abolished in conditional TrkB knockouts [].Furthermore, mice exhibiting decreased BDNF signaling developed fewer recurrent seizures after an initial kainic acid-induced status epilepticus [].Together, these data lead to the hypothesis of a positive feedback loop, in which seizures increase BDNF expression and in turn aggravate the severity of seizures [].
For devising antiepileptic therapies, Binder et al. [] emphasized the importance of understanding the association between BDNF and hyperexcitability in animal models of epilepsy.In this study, hippocampal BDNF was present in eff ective levels in epileptic rats treated with PTZ to induce seizures.In NPY-treated animals, the decrease was not statistically signifi cant, although there was an apparent decrease in BDNF levels compared with those in the group with PTZinduced seizures.Corvino et al. [] investigated the correlation between exogenously administered NPY and BDNF and found that NPY caused an increase in BDNF mRNA levels.However, BDNF levels were evaluated only after - days to show the eff ects of NPY in trimethyl-induced hippocampal neurodegeneration and temporal lobe epilepsy.In our present study, to determine BDNF levels, we collected tissues  h after administering NPY.Gelfo et al. [] found that NPY administered ip causes a decrease in BDNF levels in rat hypothalamus but not hippocampus.BDNF production will in turn induce NPY production, considering the current view that BDNF activates intracellular processes leading to transcriptional activation of NPY [, -].Th ose results suggest that Y receptors play an important role in the cross-talk between BDNF and NPY and are responsible, at least in part, for the control of BDNF production [].In the present study, administration of NPY may have caused an apparent decrease in endogenous BDNF levels through feedback inhibition.Because epilepsy is a chronic disease leading to neurodegeneration, chronic administration of NPY is necessary to clearly demonstrate the eff ects of NPY on BDNF levels, and such experiments will be more informative.Further investigations are required to elucidate the link between BDNF and NPY in epileptic brains.

CONCLUSION
In conclusion, we demonstrated that single dose treatment with NPY decreased the MDA and NO levels and increased GSH levels in rats with PTZ-induced epileptic seizures.Consideration of the balance between oxidative and nitrosative metabolism strengthens the possibility that NPY exerts neuroprotective effects.

FIGURE 3 .
FIGURE 3. Eff ect of Neuropeptide Y on GSH levels in the hippocampus of Wistar rats.GSH levels were signifi cantly lower in the SF + PTZ group than in the SF + SF and the NPY + PTZ groups (respective values: **p < 0.001, **p < 0.001).

FIGURE 4 .
FIGURE 4.Eff ect of Neuropeptide Y on BDNF levels in the hippocampus of Wistar rats.BDNF levels were signifi cantly higher in the SF + PTZ group than in the SF + SF group (**p < 0.001).
HALE MARAL KIR ET AL.: EFFECTS OF SINGLEDOSE NEUROPEPTIDE Y ON LEVELS OF HIPPOCAMPAL BDNF, MDA, GSH, AND NO IN A RAT MODEL OF PENTYLENETETRAZOLEINDUCED EPILEPTIC SEIZURE Bosn J Basic Med Sci 2013; 13 (4): 243-247 SF + PTZ group than in the SF + SF and SF + NPY groups (. ± ., . ± ., . ± . nmol/ mg protein, p < . and p < ., respectively) (Figure).Hippocampal NO levels were signifi cantly higher in the SF + PTZ group than in the SF + SF and SF + NPY groups (. ± ., . ± ., . ±  μmol/ mg protein, p < ., respectively) (Figure).In contrast the mean GSH levels were significantly lower in the SF + PTZ group than in the SF + SF, and SF + NPY FIGURE 1. Eff ect of Neuropeptide Y on MDA levels in the hippocampus of Wistar rats.MDA levels were signifi cantly higher in the SF + PTZ group than in the SF + SF and the NPY + PTZ groups (respective values: *p < 0.05, **p < 0.001).FIGURE 2.Eff ect of Neuropeptide Y on NO levels in the hippocampus of Wistar rats.NO levels were signifi cantly higher in the SF + PTZ group than in the SF + SF and the NPY + PTZ groups (respective values: **p < 0.001, **p < 0.001).groups.(.±.,.± ., . ± . nmol/ mg protein, p < . and p < ., respectively) (Figure).The mean BDNF levels were significantly higher in the SF + PTZ group than in the SF + SF group (.±.,. ± . ng/g protein, p < ., respectively)(Figure ).The intra-and inter-assay coefficients of variation for BDNF were .  and . , respectively.