Asymmetry of limbic structure ( hippocampal formation and amygdaloidal complex ) at PTSD

Defining exact position of weak anatomic function which is find in a base of neurological and psychiatric disorder is just became the subject of intensive research interest. For this purposes it is important to implement structural and functional MRI techniques, also for further lightening and seeing subject of this work, more concretely connected to PTSD. Therefore, exactly MRI gives most sensitive volumetric measuring of hippocampal formation and amygdaloidal complex. The goal of this work was to research asymmetry of hippocampal formation and amygdaloidal complex to the PTSD patients. Results showed that at the axial slice length of hippocampal formation on the left and right side of all patients are significantly asymmetric. At the sagittal slice from the left side of hippocampal formation is in many cases longer than right about 50 %. At the coronal slice, there are no significant differences toward patient proportion according to symm./asymm. of the hippocampal formation width at the right and left side. Difference in volume average of hippocampal formation between right and left side for axial and coronal slice is not statistically significant, but it is significant for sagittal slice. In about amygdaloidal complex patients with PTSD toward symm./asymm. Amygdaloidal complex at the right and left side of axial and sagittal slice in all three measurement shows asymmetry, what is especially shown at sagittal slice. Difference in average length of amygdaloidal complex at the right and left side is not statistically significant for no one slice. Therefore, results of a new research that are used MRI, showed smaller hippocampal level at PTSD (researched by Van der Kolka 1996, Pitman 1996, Bremner et al., 1995.). Application of MRI technique in research of asymmetry of hippocampal formation and amygdaloidal complex, which we used in our research, we recommend as a template for future researches in a sense of lightening anatomic function that is a base of neuropsychiatric disorders.


Introduction
Concerning development concept of PTSD since 1980 by American society of psychiatrics, officially is accepted what even laics knows, that extreme stress can lead to continuous psychiatric disorder.World health organization (WHO) PTSD entering in their 10 revision of International illness classification (IIK 10) and related health problems.Last years became clear and in specialist and scientific societies accepted that PTSD is not the only shape of psychopathological disorder that is caused by stress.Today it is find that in around 20 % people who's passing through heavy stresses, heavy psychiatric disorders has been developed, or that in a around 18 % of ambulance psychiatric patients psychiatric disorder for which they are contacting psychiatrics, are results of life trauma.
Hypersensibility, avoiding / emotional weakening, and repeatedly happening situations with hard remembrance of trauma are defined signs of PTSD.Authors presume that many of PTSD symptoms are result of limbic structure hyperactivity, which can through their many projections in to prefrontal cortex contribute to dysfunction of this system, and as results of that expect the loss in performance with frontal functioning.This hyperactivity can be physiological correlate of fear structure knowledge (hypothesis made by Foa, Feske, Murdock, Kozak I McCarthy, 1991.), which are contagiously activated and can be leader of emotional abnormality, as sudden shows of hyper sensibility and sense of horror.Authors conclude that what ever neuropathological mechanism in sense is, no matter is this is prefrontal cortical pathology, lower function of limbic system, or most probably some combination of these two, their follows OR, OI and ONPSU damages in PTSD clearly contain involvement of frontal system.

Materials and Methods
As a material for construction of this study, we used 10 MRI scan patients with PTSP, where we have cognately function damage.Methods of work include measuring the size of hippocampal formations and amygdaloidal complex in all three projections (axial, coronal and sagittal) 10 patients with PTSP, where we have cognately functional damage.

Asymmetry of limbic structure (hippocampal formation and amygdaloidal complex ) at PTSD
Aida Sara~-Had`ihalilovi}, Faruk Dilberovi} Department of Anatomy, Faculty of Medicine, University of Sarajevo, ^ekaluša 90 MRI scans are done on MAGNET IMPACT SIEMENS 1.0 TESLA in T1 relaxation (TR 500 -600 / TE 15 / field of view 180 x 260, the fatness layer SL 5 mm) and T2 relaxation (TR 4000 / TE 90 field of view 188 x 250 for axial and 173 x 230 for coronal, 210 x 240 for sagittal scans in 5 mm layer).Dual sequences are used PD and T2.In PD TR is 4000, and TE 22.We used a headneck spiral, as well as a head spiral.
For the size measurement of amygdaloidal complex and hippocampal formation, and their comparison from right to left, we used a program of evaluation-distance on the MRI from the Institute of Radiology of Clinical Centre in Sarajevo.We tested 10 patients with PTSP, approximate age 49.9 with standard deviation of 4.62 years.
The size of hippocampal formation is measurement in all three projections: horizontal (axial), frontal (coronal) and sagittal from right and left.The size of amygdaloidal complex is measurement in two projections: horizontal (axial) and sagittal.In axial projections, we are measurement anterior -posterior and lateral -medial diameter of amygdaloidal complex.We do not measurement the amygdaloidal complex in coronal projections, because it is not possible his clear diffraction from the other cerebral structures.All values of hippocampal formation and amygdaloidal complex are given in centimetres.
For hippocampal, formation and amygdaloidal complex in all three projections (axial, coronal and sagittal) are met in:  According to basis of analyze of our results linked to size of hippocampal formation in all three projection (axial, coronary and sagittal), in a group of patient with PTSD, we can observe on a following way: 1. On to axial slice length of hippocampal formation on left and right side on all patients are significantly asymmetric.On to sagittal slice from the left side, the hippocampal formation is in many cases longer than right -50 %.On coronary slice, there are no significant differences toward proportion patient according to symm./asymm. of width of hippocampal formation on the left and right side.
2. Difference in average size of hippocampal formation between left and right side for axial and coronary slice is not statistically significant, but it is significant for sagittal slice.
According to analysis of our results connected to size of amygdaloidal complex in two projections (axial: ant.post. in addition, lat.-med.diameter and sagittal), in a group of patient with PTSD, we can conclude that: 1. Patient with PTSD according to symm./ asymm. of amygdaloidal complex on the left and right side of axial and sagittal slice in all 3 measurement shows asymmetry, what is mostly present on sagittal slice.
2. Difference in average length of amygdaloidal complex on the left and right side is not statistically significant for none of the slices.

Conclusions
On basis of our analysis, we can conclude: 1. Every one of analyzed asymmetry shows the same characteristics in the group, in witch we emphasize variations.
2. We also emphasize the importance of presence of conciseness in individual characteristics of every one of the parameters in the shading light on asymmetry of hippocampal formation and amygdaloidal complex.
3. We have to be careful about what projection we are refusing to as being watched hippocampal formation and amygdaloidal complex because the results will depend on that.We can suggest the prospective studies in more projections because of the value of the statistically significant conclusions.
5. Usage of MRI techniques in examining the asymmetry of hippocampal formation and amygdaloidal complex, that we used, we suggest as the studies in the future research in the sense of the shading light on the anatomical functions that are based on neuropsychiatry dysfunctions.

Results MRI analysis in pacients with PTSD Picture
1 Axial MRI scans -the slice on a hippocampal formation level Picture 2 Axial MRI scans -length of hippocampal formation from the right and the left side

Table 1
Shows patients with PTSD towards simmetry/asimmetry hippocampal formation from right and left on the axial, coronal and sagittal slice

Table 2
Approximate size of the hippocampal formation on the right and left side on the axial, coronal and sagittal slice on patient with PTSD Length of hippocampal formation on the left and right side of axial slice are on all patient significantly asymmetric.Difference in average size of hippocampal formation between right and left side is not statistically significant for axial and coronary slice, but it is significant for sagittal slice.
Value of Chi -square test is: ChiSq = 10, level of assurmen.is p < 0.01.Length of hippocampal formation on the left and right side of sagittal slice is not statistically significant on the level of reliability p < 0.05.Value of ChiSq = 3.6.(Sign.for level p < 0.10).At the coronary slice, there are no significant differences in patient proportion toward symm/asymm of width hippocampal formation with right and left side.

Table 3
Shows patients with PTSD towards symmetry /asymmetry amygdaloidal complex from right and left on the axial, coronal and sagittal slice On all three measurements, there are asymmetry in length of right and left side, and what is especially signed on sagittal slice.Value Chi -square test for sagittal slice is ChiSq = 10, level of reliability is p < 0.01.Difference in average length of right and left side is not statistically significant for any case.Values of t -tests are:

Table 4
Approximate length of the amygdaloidal complex on the right and left side on the axial and sagittal slice on patient with PTSD