Polymorphisms in DNA repair genes XRCC2 and XRCC3 risk of gastric cancer in Turkey

  • İlhami Gok Department of Bioengineering, Faculty of Engineering & Architecture Kafkas University, 36100 Kars/ Turkey Phone: Fax : +90 474 225 12 82
  • Meryem Baday Departments of Biology, Faculty of Science &Literature, Kafkas University , Kars, Turkey
  • Suleyman Cetinkunar Departments of Surgery , Adana Numune Training and Research Hospital, Adana , Turkey
  • Kemal Kilic Departments of Surgery, Kartal Training and Research Hospital ,İstanbul , Turkey
  • Bulent Caglar Bilgin Department of Surgery , School of Medicine , Kafkas University, Kars , Turkey
Keywords: gastric cancer, XRCC2 and XRCC3 genes, polymorphism, Turkey

Abstract

We studied the prevalence of polymorphisms in genes XRCC2 and XRCC3 in stomach cancer patients who lived in North Eastern Turkey. A total of 61 cancer patients and 78 controls were included in this study. Single nucleotide changes were studied in XRCC2 and XRCC3 genes at locus Arg188His and Thr241Met. Blood samples were taken from the patients and controls, and DNA was isolated. The regions of interest were amplified using a polymerase chain reaction method. After amplification, we used restriction enzymes (HphI and NcoI) to digest the amplified product. Digested product was then run through gel electrophoresis. We identified changes in the nucleotides in these specific regions. It was found that the Arg188His polymorphism of the XRCC2 gene was about 39% (24 out of the 61) among cancer patients. However, only 15% (12 out of 78) of the control group indicated this polymorphism. We also observed that 18 of the 61 cancer patients (29%) carried the Thr241Met polymorphism of the XRCC3 gene whereas 11 of the 78 (14%) individuals in the control group had the polymorphism. Our results showed a significant difference in polymorphism ratios between the cancer patients and health control group for the regions of interest. This result clearly showed that these polymorphisms increase the risk of stomach cancer and might be a strong marker for early diagnosis of gastric cancer.

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Author Biographies

İlhami Gok, Department of Bioengineering, Faculty of Engineering & Architecture Kafkas University, 36100 Kars/ Turkey Phone: Fax : +90 474 225 12 82
Human genetics, Molecular Biology , Bioengineering
Meryem Baday, Departments of Biology, Faculty of Science &Literature, Kafkas University , Kars, Turkey
Molecular Biology, genetics
Suleyman Cetinkunar, Departments of Surgery , Adana Numune Training and Research Hospital, Adana , Turkey
Oncology, Surgery
Kemal Kilic, Departments of Surgery, Kartal Training and Research Hospital ,İstanbul , Turkey
Surgery, oncology
Bulent Caglar Bilgin, Department of Surgery , School of Medicine , Kafkas University, Kars , Turkey
general surgery
Published
2014-09-13
How to Cite
1.
Gok İlhami, Baday M, Cetinkunar S, Kilic K, Bilgin BC. Polymorphisms in DNA repair genes XRCC2 and XRCC3 risk of gastric cancer in Turkey. Bosn J of Basic Med Sci [Internet]. 2014Sep.13 [cited 2019Jul.17];14(4):214-8. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/214
Section
Molecular Biology