Meticillin-resistant Staphylococcus aureus isolated from Iranian hospitals: virulence factors and antibiotic resistance properties

  • Hassan Momtaz Department of Microbiology, Shahrekord Branch, Islamic Azad University
  • Laleh Hafezi Post graduated of Master of Science of Microbiology, Shahrekord Branch, Islamic Azad University
Keywords: Staphylococcus aureus, SCCmec types, Panton-Valentine Leukocidin, antibiotic resistance properties, Iranian hospitals

Abstract

Staphylococcus aureus is an important opportunistic pathogen responsible for a variety of diseases. Indiscriminate prescription of antibiotics caused severe antibiotic resistance especially against commonly used drugs. The present investigation was carried out to study the distribution of Panton-Valentine Leukocidin gene, SCCmec types and antibiotic resistance properties of meticillin-resistant Staphylococcus aureus isolated from Iranian hospitals. A total of 132 clinical specimens were collected from two major Iranian hospitals. Samples were cultured and their positive results were subjected to several PCR methods. The patterns of antibiotic resistance were studied using the disk diffusion method. We found that 66 out of 132 samples (50%) were positive for Staphylococcus aureus. The most commonly infected samples were superficial and surgical wounds (66.12%). The incidence of mecA, tetK, ermA, ermC, tetM, aacA-D, linA, msrA, vatA, vatC and vatB antibiotic resistance genes were 80.30%, 34.84%, 30.30%, 25.75%, 24.24%, 19.69%, 7.57%, 7.57%, 6.06%, 3.03% and 1.51%, respectively. Totally, 40.90% of isolates harbored the Panton-Valentine Leukocidin gene. Of 53 mec positive strains, the distribution of SCCmec V, SCCmec III, SCCmec IVa, SCCmec IVc and SCCmec IVb were 28 (52.83%), 13 (24.52%), 6 (11.32%), 4 (7.54%) and 2 (3.77%), respectively. All isolates were resistant to penicillin, cephalothin, cefazoline and ceftriaxone. The high levels of Staphylococcus aureus resistance against commonly used antibiotics as well as high presence of SCCmec types of meticillin-resistant virulent strains of Staphylococcus aureus suggest that infections with these strains require more advanced hospital care with emerging demand for novel antibiotics.

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Published
2014-10-05
How to Cite
1.
Momtaz H, Hafezi L. Meticillin-resistant Staphylococcus aureus isolated from Iranian hospitals: virulence factors and antibiotic resistance properties. Bosn J of Basic Med Sci [Internet]. 2014Oct.5 [cited 2019Jul.17];14(4):219-26. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/219
Section
Microbiology