CD44 silencing decreases the expression of stem cell-related factors induced by transforming growth factor β1 and tumor necrosis factor α in lung cancer: Preliminary findings

Authors

  • Fariz Nurwidya Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan; Department of Pulmonology and Respiratory Medicine, Universitas Indonesia Faculty of Medicine, Jakarta, Indonesia http://orcid.org/0000-0002-8379-9510
  • Fumiyuki Takahashi Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
  • Motoyasu Kato Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
  • Hario Baskoro Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
  • Moulid Hidayat Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
  • Aditya Wirawan Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
  • Kazuhisa Takahashi Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan

DOI:

https://doi.org/10.17305/bjbms.2017.1966

Keywords:

CD44, CXCR4 receptor, Oct4, POU5F1, non-small cell lung cancer cells, NSCLC, C-X-C chemokine receptor type 4, octamer-binding transcription factor 4

Abstract

The mechanism underlying increased concentrations of cancer stem cell (CSC)-associated factors in non-small cell lung cancer (NSCLC) cells treated with transforming growth factor β1 (TGFβ1) and tumor necrosis factor α (TNFα), is still not clear. The purpose of this study was to investigate the possible role of CD44 in the regulation of CSC-associated genes, by analyzing the effect of CD44 knockdown on their expression. A549, a NSCLC cell line that expresses CD44 antigen, was treated with TGFβ1 and TNFα. Small-interfering ribonucleic acid (siRNA) that specifically targets the CD44 gene was used to knockdown the expression of CD44 in A549. The gene expressions of CD44, CXCR4, POU5F1 (octamer-binding transcription factor 4 [Oct4]), PROM1, NANOG, c-Myc, KLF4, and SOX2, as well as of CDH1 (E-cadherin), CDH2 (N-cadherin), VIM (vimentin), and FN1 (fibronectin) were analyzed in A549 cells by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Cell morphology was observed using light microscopy. After TGFβ1/TNFα treatment, increased expressions of CXCR4 and POU5F1 were detected. Silencing of CD44 gene expression was confirmed by RT-qPCR. The knockdown of CD44 decreased the CXCR4 and POU5F1 gene expressions in TGFβ1/TNFα-treated A549 cells. However, the silencing of CD44 did not affect the morphology of TGFβ1/TNFα-treated A549 cells nor it reversed epithelial-mesenchymal transition (EMT) gene signature induced by TGFβ1/TNFα in A549 cells. Our preliminary findings suggest that the CD44 gene may have a role in regulating CXCR4 and POU5F1 gene expressions, independently of the EMT signaling pathway.

Author Biographies

  • Fariz Nurwidya, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan; Department of Pulmonology and Respiratory Medicine, Universitas Indonesia Faculty of Medicine, Jakarta, Indonesia

    Department of Respiratory Medicine

    Department of Pulmonology and Respiratory Medicine

  • Fumiyuki Takahashi, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Department of Respiratory Medicine

  • Motoyasu Kato, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
    Department of Respiratory Medicine
  • Hario Baskoro, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
    Department of Respiratory Medicine
  • Moulid Hidayat, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
    Department of Respiratory Medicine
  • Aditya Wirawan, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
    Department of Respiratory Medicine
  • Kazuhisa Takahashi, Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan
    Department of Respiratory Medicine

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CD44 silencing decreases the expression of stem cell-related factors induced by transforming growth factor β1 and tumor necrosis factor α in lung cancer: Preliminary findings

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20-08-2017

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CD44 silencing decreases the expression of stem cell-related factors induced by transforming growth factor β1 and tumor necrosis factor α in lung cancer: Preliminary findings. Biomol Biomed [Internet]. 2017 Aug. 20 [cited 2024 Mar. 29];17(3):228-34. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/1966