Nitric oxide as a potential biomarker in inflammatory bowel disease

  • Nesina Avdagić Department of Physiology, Faculty of Medicine, University of Sarajevo
  • Asija Zaćiragić Department of Physiology, Faculty of Medicine, University of Sarajevo
  • Nermina Babić Department of Physiology, Faculty of Medicine, University of Sarajevo
  • Mirsada Hukić Department of Physiology, Faculty of Medicine, University of Sarajevo
  • Mensura Šeremet Department of Physiology, Faculty of Medicine, University of Sarajevo
  • Orhan Lepara Department of Physiology, Faculty of Medicine, University of Sarajevo
  • Emina Nakaš-Ićindić Department of Physiology, Faculty of Medicine, University of Sarajevo
Keywords: nitric oxide, inflammatory bowel disease, ulcerative colitis, Crohn’s disease

Abstract

The aim of this study was to investigate changes in serum nitric oxide (NO) concentration in inflammatory bowel diseases (IBD) patients and its use as potential biomarker in differential diagnosis of ulcerative colitis (UC) and Crohn's disease (CD) and in disease activity assessment. In 60 patients of both genders - 30 with ulcerative colitis and 30 with Crohn's disease - and 30 controls serum nitric oxide concentration was determined by measuring nitrite concentration, a stable metabolic product of NO with oxygen. Conversion of nitrates (NO3-) to nitrites (NO2-) was done with elementary zinc. The nitrite concentration was determined by classic colorimetrical Griess reaction. Median serum NO concentration was statistically different (p=0,0005) between UC patients (15.25 µmol/L; 13.47 - 19.88 µmol/L), CD patients (14.54 µmol/L; 13.03 -16.32 µmol/L) and healthy controls (13.29 µmol/L; 12.40 - 13.92 µmol/L). When active UC and CD patients were compared with inactive UC and CD patients respectively a significant difference in serum NO level was found (p=0.0005). With a cut-off level of 17.39 µmol/L NO had a sensitivity of 100% and a specificity of 100% in discriminating between active and inactive UC patients. With cut-off value of 14.01 µmol/L serum NO level had a sensitivity of 88% and a specificity of 69% in distinguishing between patients with active CD and inactive CD. Serum NO concentration is a minimally invasive and rapid tool for discriminating between active and inactive IBD patients and could be used as useful biomarker in monitoring of disease activity in IBD patients.

Downloads

Download data is not yet available.
Published
2013-02-20
How to Cite
1.
Avdagić N, Zaćiragić A, Babić N, Hukić M, Šeremet M, Lepara O, Nakaš-Ićindić E. Nitric oxide as a potential biomarker in inflammatory bowel disease. Bosn J of Basic Med Sci [Internet]. 2013Feb.20 [cited 2019Dec.9];13(1):5-. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/2402
Section
Biochemistry

Most read articles by the same author(s)