Molecular – genetic variance of RH blood group system within human population of Bosnia and Herzegovina

  • Lejla Lasić Institute for Genetic Engineering and Biotechnology Sarajevo
  • Naida Lojo-Kadrić Institute for Genetic Engineering and Biotechnology Sarajevo
  • Elma Silajdžić Institute for Genetic Engineering and Biotechnology Sarajevo
  • Lejla Pojskić Institute for Genetic Engineering and Biotechnology Sarajevo
  • Rifat Hadžiselimović Institute for Genetic Engineering and Biotechnology Sarajevo
  • Naris Pojskić Institute for Genetic Engineering and Biotechnology Sarajevo
Keywords: RHD gene, RHCE gene, frequency, Rh blood group system, allele specific PCR method

Abstract

There are two major theories for inheritance of Rh blood group system: Fisher - Race theory and Wiener theory. Aim of this study was identifying frequency of RHDCE alleles in Bosnian - Herzegovinian population and introduction of this method in screening for Rh phenotype in B&H since this type of analysis was not used for blood typing in B&H before. Rh blood group was typed by Polymerase Chain Reaction, using the protocols and primers previously established by other authors, then carrying out electrophoresis in 2-3% agarose gel. Percentage of Rh positive individuals in our sample is 84.48%, while the percentage of Rh negative individuals is 15.52%. Inter-rater agreement statistic showed perfect agreement (K=1) between the results of Rh blood system detection based on serological and molecular-genetics methods. In conclusion, molecular - genetic methods are suitable for prenatal genotyping and specific cases while standard serological method is suitable for high-throughput of samples.

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Published
2013-02-20
How to Cite
1.
Lasić L, Lojo-Kadrić N, Silajdžić E, Pojskić L, Hadžiselimović R, Pojskić N. Molecular – genetic variance of RH blood group system within human population of Bosnia and Herzegovina. Bosn J of Basic Med Sci [Internet]. 2013Feb.20 [cited 2019Apr.22];13(1):10-3. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/2403
Section
Molecular Biology