Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour

  • Predrag Jovanović Ophthalmology Clinic, Clinical Center
  • Lepša Žorić Department of Ophthalmology, Faculty of Medicine
  • Ivan Stefanović Institute for Eye Diseases, Clinical Center of Serbia
  • Boban Džunić Ophthalmology Clinic, Clinical Center
  • Jasmina Djordjević-Jocić Ophthalmology Clinic, Clinical Center
  • Marija Radenković Ophthalmology Clinic, Clinical Center
  • Maja Jovanovic Clinic for Infective Diseases
Keywords: glaucoma, oxidative stress, ischaemia, lactate dehydrogenase, lactates


Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma.

Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group).

The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p<0,001).

The increased activity of the examined biochemical parameters in the aqueous humour of the POAG patients points to the fact that other mechanisms, besides IOP, have a role in glaucoma pathogenesis.


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How to Cite
Jovanović P, Žorić L, Stefanović I, Džunić B, Djordjević-Jocić J, Radenković M, Jovanovic M. Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour. Bosn J of Basic Med Sci [Internet]. 2010Feb.20 [cited 2019Jun.19];10(1):83-8. Available from:

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