Proximal convoluted tubules of the rats kidney--a stereological analysis

Selma Aličelebić

doi:10.17305/bjbms.2003.3568

Authors

Selma Aličelebić Institute of Histology and Embryology, University of Sarajevo, School of Medicine

DOI:

https://doi.org/10.17305/bjbms.2003.3568

Keywords:

kidney, proximal convoluted tubule, stereology, rat

Abstract

Background and Purpose: The aim of this work was to order quantitative parameters of the proximal convoluted tubules (PCT) in the normal rat kidney cortex. Volume density (V_V), both surface and specific surface density toward interstitium (S_Vi and S_Vi/V_V) and toward lumen (S_Vl and S_Vl/V_V) and thickness (T) of tubules epithelium have been stereologically ordered.

Material and Methods: Stereologically were analysed 170 test fields by lattice L36 on the paraffin sections of the three adult male Wistar rats kidney dyeing by PAS-method.

Results: The mean values of the variables analysed were V_v=76.4% ±0.012; S_vi=0.056pm-1 ±0.004; S_vl=0.028pm-1 ±0.003; S_vi/V_v=0.073pm-1 ±0.003; S_vl/V_v=0.037pm-1 ±0.005; T=18,26pm ±0.897.

Conclusions: Stereological methods are making a very valuable contribution to science over recent years. We have used unbiased stereological counting methods to obtain objective quantitative parameters of the PCT epithelium in the normal rats’ kidney cortex.