DL-2-amino-3-phosphonopropionic acid protects primary neurons from oxygen-glucose deprivation induced injury

Di Cui; Jun Xu; Quanyi Xu; Guokun Zuo

doi:10.17305/bjbms.2016.1553

Authors

Di Cui Department of Robot and Neuro-Rehabilitation, Ningbo Institute of Industrial Technology, Chinese Academy of Sciences, Ningbo, China; Department of Computer Science, Ningbo University of Technology, Ningbo, China
Jun Xu Department of Neurology, Yinzhou Second Hospital of Ningbo, Ningbo, China
Quanyi Xu Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, China
Guokun Zuo Department of Robot and Neuro-Rehabilitation, Ningbo Institute of Industrial Technology, Chinese Academy of Sciences, Ningbo, China

DOI:

https://doi.org/10.17305/bjbms.2016.1553

Keywords:

DL-2-amino-3-phosphonopropionic acid, cerebral infarction, oxygen-glucose deprivation, neuron viability, apoptosis

Abstract

Cerebral infarction is a type of ischemic stroke and is one of the main causes of irreversible brain damage. Although multiple neuroprotective agents have been investigated recently, the potential of DL-2-amino-3-phosphonopropionic acid (DL-AP3) in treating oxygen-glucose deprivation (OGD)-induced neuronal injury, has not been clarified yet. This study was aimed to explore the role of DL-AP3 in primary neuronal cell cultures. Primary neurons were divided into four groups: (1) a control group that was not treated; (2) DL-AP3 group treated with 10 μM of DL-AP3; (3) OGD group, in which neurons were cultured under OGD conditions; and (4) OGD + DL-AP3 group, in which OGD model was first established and then the cells were treated with 10 μM of DL-AP3. Neuronal viability and apoptosis were measured using Cell Counting Kit-8 and flow cytometry. Expressions of phospho-Akt1 (p-Akt1) and cytochrome c were detected using Western blot. The results showed that DL-AP3 did not affect neuronal viability and apoptosis in DL-AP3 group, nor it changed p-Akt1 and cytochrome c expression (p > 0.05). In OGD + DL-AP3 group, DL-AP3 significantly attenuated the inhibitory effects of OGD on neuronal viability (p < 0.001), and reduced OGD induced apoptosis (p < 0.01). Additionally, the down-regulation of p-Akt1 and up-regulation of cytochrome c, induced by OGD, were recovered to some extent after DL-AP3 treatment (p < 0.05 or p < 0.001). Overall, DL-AP3 could protect primary neurons from OGD-induced injury by affecting the viability and apoptosis of neurons, and by regulating the expressions of p-Akt1 and cytochrome c.

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Author Biographies

Di Cui, Department of Robot and Neuro-Rehabilitation, Ningbo Institute of Industrial Technology, Chinese Academy of Sciences, Ningbo, China; Department of Computer Science, Ningbo University of Technology, Ningbo, China

Department of Robot and Neuro-Rehabilitation;
Department of Computer Science
Jun Xu, Department of Neurology, Yinzhou Second Hospital of Ningbo, Ningbo, China

Department of Neurology
Quanyi Xu, Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, China

Department of Pharmacy
Guokun Zuo, Department of Robot and Neuro-Rehabilitation, Ningbo Institute of Industrial Technology, Chinese Academy of Sciences, Ningbo, China

Department of Robot and Neuro-Rehabilitation

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