Time course of global gene expression alterations in Candida albicans during infection of HeLa cells

Yi-Bing Lan; Yi-Zhou Huang; Fan Qu; Juan-Qing Li; Lin-Juan Ma; Jie Yan; Jian-Hong Zhou

doi:10.17305/bjbms.2017.1667

Authors

Yi-Bing Lan Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yi-Zhou Huang Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China
Fan Qu Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China
Juan-Qing Li Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lin-Juan Ma Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China
Jie Yan Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Jian-Hong Zhou Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

DOI:

https://doi.org/10.17305/bjbms.2017.1667

Keywords:

Candida albicans, infection, differential gene expression, HeLa cells

Abstract

Candida albicans (C. albicans) is an opportunistic fungus that quickly adapts to various microniches. It causes candidiasis, a common fungal infection for which the pathogenic mechanism has not been elucidated yet. To explore the pathogenic mechanism of candidiasis we used several methods, including microscopic observation of morphological changes of HeLa cells and fungus, analysis of differentially expressed genes using gene chips, and a series of biological and bioinformatic analyses to explore genes that are possibly involved in the pathogenesis of C. albicans. During the C. albicans infection, significant morphological changes of the fungus were observed, and the HeLa cells were gradually destroyed. The gene chip experiments showed upregulated expression of 120 genes and downregulated expression of 178 genes. Further analysis showed that some genes may play an important role in the pathogenesis of C. albicans. Overall, morphological variation and adaptive gene expression within a particular microniche may exert important effects during C. albicans infections.

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Author Biographies

Yi-Bing Lan, Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Gynecology
Yi-Zhou Huang, Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Gynecology
Fan Qu, Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Gynecology
Juan-Qing Li, Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Gynecology
Lin-Juan Ma, Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Gynecology
Jie Yan, Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Medical Microbiology and Parasitology
Jian-Hong Zhou, Department of Gynecology, The Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China

Department of Gynecology

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