Dose- and time-dependent effects of clodronate on orthodontic tooth movement

Enita Nakaš; Tomislav Lauc; Alisa Tiro; Vildana Džemidžić; Amila Zukanović; Miljenko Franić; Vladimir Ivković

doi:10.17305/bjbms.2017.1715

Authors

Enita Nakaš Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Tomislav Lauc Department for Dental Medicine, Faculty of Medicine, University of Osijek, Osijek, Croatia; Department for Anthropology, Faculty for Social Studies and Humanities, University of Zagreb, Zagreb, Croatia http://orcid.org/0000-0002-4163-0856
Alisa Tiro Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Vildana Džemidžić Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Amila Zukanović Department of Preventive Dentistry, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Miljenko Franić Department of Orthopedics and Physical Rehabilitation, Faculty of Medicine, University of Osijek, Osijek, Croatia
Vladimir Ivković Laboratory for Neuroimaging and Integrative Physiology, Neural Systems Group, Department of Psychiatry, Harvard Medical School, Massachusetts General Hospital, Boston, USA

DOI:

https://doi.org/10.17305/bjbms.2017.1715

Keywords:

Histology, tooth movement, pharmacology, bone biology, clodronate, bisphosphonate

Abstract

Orthodontic tooth movement is the result of bone remodeling that occurs in periodontal ligament and alveolar bone tissue as a response to mechanical loading of the tooth. The aim of this study is to investigate the time- and dose-response effects of locally administered clodronate on tooth movement. Sixty Wistar rats were randomly assigned to 4 groups of 15 specimens: E1 - application of 10 mMol of clodronate in 3-day intervals; E2 - application of 2.5 mMol of clodronate in 3-day intervals; E3 - application of 10 mMol of clodronate in 7-day intervals; E4 - application of 2.5 mMol of clodronate in 7-day intervals. A 50 μL clodronate solution was injected into a subperiosteal area to the right maxillary incisor. The left maxillary incisor served as a control, with an injection of saline solution. In 3-day interval application regime, there was no effect of clodronate dosing on tooth movement. In 7-day interval application regime, decreased tooth movement was observed with 10 mMol compared with 2.5 mMol clodronate concentration. However, decreased tooth movement was also observed when 2.5 mMol of clodronate was applied in 7-versus 3-day intervals. Conversely, no difference was observed when 10 mMol concentration was applied in 3- versus 7-day intervals. When clodronate is applied subperiosteally in the root area, it decreases the tooth movement. Tooth movement is impeded by the higher clodronate dosing, as well as by shorter application interval even with lower dosing. The purpose of future trials should, therefore, be to determine a safe therapeutic dose/interval application of clodronate in humans and their potential side effects.

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Author Biographies

Enita Nakaš, Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Department of Orthodontics
Tomislav Lauc, Department for Dental Medicine, Faculty of Medicine, University of Osijek, Osijek, Croatia; Department for Anthropology, Faculty for Social Studies and Humanities, University of Zagreb, Zagreb, Croatia

Department for Dental Medicine; Department for Anthropology
Alisa Tiro, Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Department of Orthodontics
Vildana Džemidžić, Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Department of Orthodontics
Amila Zukanović, Department of Preventive Dentistry, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Department of Preventive Dentistry
Miljenko Franić, Department of Orthopedics and Physical Rehabilitation, Faculty of Medicine, University of Osijek, Osijek, Croatia

Department of Orthopedics and Physical Rehabilitation
Vladimir Ivković, Laboratory for Neuroimaging and Integrative Physiology, Neural Systems Group, Department of Psychiatry, Harvard Medical School, Massachusetts General Hospital, Boston, USA

Department of Psychiatry

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