Prevalence of F5 1691G>A, F2 20210G>A, and MTHFR 677C>T polymorphisms in Bosnian women with pregnancy loss

Emir Mahmutbegović; Damir Marjanović; Edin Medjedović; Nevena Mahmutbegović; Serkan Dogan; Amina Valjevac; Ewa Czerska; Anna Pawińska-Matecka; Agnieszka Madlani; Grażyna Adler

doi:10.17305/bjbms.2017.1954

Authors

Emir Mahmutbegović Institution of Health Protection of Women and Motherhood Canton Sarajevo, Sarajevo, Bosnia and Herzegovina
Damir Marjanović International Burch University, Sarajevo, Bosnia and Herzegovina; Institute for Anthropological Research, Zagreb, Croatia
Edin Medjedović Gynecology Clinic, Clinical Center of University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Nevena Mahmutbegović Neurology Clinic, Clinical Center of University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Serkan Dogan International Burch University, Sarajevo, Bosnia and Herzegovina
Amina Valjevac Laboratory for Molecular Medicine, Center for Genetics, Medical Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Ewa Czerska Central Laboratory, Regional Hospital, Szczecin, Poland
Anna Pawińska-Matecka Central Laboratory, Regional Hospital, Szczecin, Poland
Agnieszka Madlani Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland
Grażyna Adler Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland http://orcid.org/0000-0001-8792-553X

DOI:

https://doi.org/10.17305/bjbms.2017.1954

Keywords:

Pregnancy loss, risk factors, polymorphisms, thrombophilia, F5, F2, MTHFR

Abstract

The relationship between genetic risk factors of thrombophilia and pregnancy loss (PL) is being discussed. The focus has been on F5 1691G>A, F2 20210G>A, and MTHFR 677C>T polymorphisms that may predispose women to microthrombosis during the stages of embryo implantation and placentation. Although, the frequencies of these polymorphisms were reported in different populations, such studies have not yet been performed in Bosnian population. In this study, we determined the prevalence of F5 G>A (rs6025), F2 G>A (rs1799963) and MTHFR C>T (rs1801133) polymorphisms in Bosnian women. A total of 154 women with PL, mean age 33 (±5.4) years, were enrolled in the study. As a control group, 154 mothers [mean age 31.4 (±6.7) years] with at least one live-born child were included. We used real-time polymerase chain reaction (PCR) to determine the frequencies of F5 G>A and F2 G>A genotypes, and PCR-restriction fragment length polymorphism (RFLP) for analyzing MTHFR C>T genotypes. The frequency of heterozygotes for F5 and F2 was significantly higher in women with venous thrombosis (VT) compared to women without VT (p = 0.047 and p = 0.001, respectively). There was no significant difference in the distribution of MTHFR genotypes and alleles between these two groups. In addition, we observed no significant differences in the genotype and allele frequencies between the group with PL and control group, for all investigated polymorphisms. The allele frequencies for 1691A (F5), 20210A (F2), and 677T (MTHFR) reported in this study are consistent with the data obtained for other European countries, however, we were not able to confirm the association between the three polymorphisms and PL in Bosnian women.

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Author Biographies

Agnieszka Madlani, Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland

Department of Gerontobiology
Grażyna Adler, Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland

Department of Gerontobiology

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