ABCB1 3435C>T and 2677G>T/A polymorphisms in Polish and Bosnian patients with Crohn’s disease – A preliminary report

Authors

  • Krzysztof Borecki Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland
  • Iwona Zawada Department of Gastroenterology, Pomeranian Medical University, Szczecin, Poland
  • Anna Pawinska-Matecka Central Laboratory, Regional Hospital, Szczecin, Poland
  • Nermin Nusret Salkic Department of Gastroenterology and Hepatology, University Clinical Center Tuzla, Tuzla, Bosnia and Herzegovina
  • Beata Karakiewicz Public Health Department, Pomeranian Medical University, Szczecin, Poland
  • Grażyna Adler Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland

DOI:

https://doi.org/10.17305/bjbms.2017.2172

Keywords:

ABCB1, Crohn’s disease, allele frequency, Poland, Bosnia and Herzegovina, 3435C>T, 2677G>T/A, single nucleotide polymorphism, SNP

Abstract

The role of ABCB1 single nucleotide polymorphisms (SNPs) in the development of Crohn’s disease (CD) remains unclear. Due to inconsistent results of several European population-based studies and limited information on populations from Poland and Bosnia and Herzegovina (BH), we conducted a preliminary association study of two main ABCB1 SNPs and CD. ABCB1 3435C>T and 2677G>T/A SNPs were analyzed in Polish and Bosnian patients with CD (n = 85 and n = 30, respectively) and controls (n = 82 and n = 30, respectively) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 3435C>T and allele-specific PCR for 2677G>A/T SNP. A deviation from Hardy-Weinberg equilibrium was found for both SNPs in Polish patients with CD, and for 2677G>A/T in Polish control group. The allele and genotype frequencies of the two ABCB1 SNPs were not significantly different between the CD patients and controls in both populations (p > 0.05). Similarly, the genotype distribution of 3435C>T and 2677G>T/A SNPs was not significantly different between Polish and Bosnian patients with CD (p > 0.05). At least one mutated ABCB1 allele was carried by 97.7% of Polish and 90.0% of Bosnian patients with CD. No association was found between the ABCB1 SNPs and CD in the two populations. In conclusion, the two ABCB1 SNPs may not contribute to CD susceptibility in the populations of Poland and BH. Further studies with larger samples in both populations are warranted.

Citations

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Author Biographies

  • Krzysztof Borecki, Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland

    Department of Gerontobiology

  • Iwona Zawada, Department of Gastroenterology, Pomeranian Medical University, Szczecin, Poland
    Department of Gastroenterology
  • Nermin Nusret Salkic, Department of Gastroenterology and Hepatology, University Clinical Center Tuzla, Tuzla, Bosnia and Herzegovina
    Department of Gastroenterology and Hepatology
  • Beata Karakiewicz, Public Health Department, Pomeranian Medical University, Szczecin, Poland
    Public Health Department
  • Grażyna Adler, Department of Gerontobiology, Pomeranian Medical University, Szczecin, Poland
    Department of Gerontobiology

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ABCB1 3435C>T and 2677G>T/A polymorphisms in Polish and Bosnian patients with Crohn’s disease – A preliminary report

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Published

20-11-2017

How to Cite

1.
ABCB1 3435C>T and 2677G>T/A polymorphisms in Polish and Bosnian patients with Crohn’s disease – A preliminary report. Biomol Biomed [Internet]. 2017 Nov. 20 [cited 2024 Nov. 5];17(4):323-7. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2172