An assessment of correlation between serum asymmetric dimethylarginine and glycated haemoglobin in patients with type 2 diabetes mellitus

Asija Začiragić; Jasminko Huskić; Nedžad Mulabegović; Nesina Avdagić; Amina Valjevac; Sabaheta Hasić; Radivoj Jadrić

doi:10.17305/bjbms.2014.2291

Authors

Asija Začiragić Department of Physiology, Faculty of Medicine, University of Sarajevo
Jasminko Huskić Department of Physiology, Faculty of Medicine, University of Sarajevo
Nedžad Mulabegović Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Sarajevo
Nesina Avdagić Department of Physiology, Faculty of Medicine, University of Sarajevo
Amina Valjevac Department of Physiology, Faculty of Medicine, University of Sarajevo
Sabaheta Hasić Department of Biochemistry, Faculty of Medicine, University of Sarajevo
Radivoj Jadrić Department of Biochemistry, Faculty of Medicine, University of Sarajevo

DOI:

https://doi.org/10.17305/bjbms.2014.2291

Keywords:

asymmetric dimethylarginine, endothelial dysfunction, glycated haemoglobin, type 2 diabetes mellitus

Abstract

Studies that investigated an association between asymmetric dimethylarginine (ADMA) and glycated haemoglobin (HbA1c) in type 2 diabetes mellitus (T2DM) have given discordant results. The aim of this study was to determine and compare serum ADMA concentration in patients with T2DM and healthy controls, and to assess correlation between ADMA and HbA1c in patients with T2DM. Serum ADMA concentration was determined by ELISA method with the use of ADMA ® - ELISA kit (DLD Diagnostics, Hamburg, Germany) and HbA1c levels were determined by an immunoturbidimetric method in 60 patients with T2DM and 60 healthy individuals matched for age and sex. Results have shown that mean serum ADMA concentration was significantly higher in T2DM patients (1.54±0.06 μmol/L) compared to mean serum ADMA concentration (0.62±0.02 μmol/L; p<0.0001) in healthy subjects. A significant, positive, correlation between serum ADMA concentration and HbA1c levels was observed (r=0.494; p<0.01) in T2DM patients. Our results suggest that there is an association between endothelial dysfunction and glycaemic control in type 2 diabetes mellitus. Possible explanation for obtained results may be oxidative stress that is increased in conditions of hyperglycaemia and it also promotes endothelial dysfunction. Larger, longitudinal studies are required that will evaluate relation between metabolic abnormalities and increased ADMA levels in patients with type 2 diabetes mellitus.