The effect of 131I-induced hypothyroidism on the levels of nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), total nitric oxide synthase (NOS) activity, and expression of NOS isoforms in rats

Jing Zhou; Gang Cheng; Hua Pang; Qian Liu; Ying Liu

doi:10.17305/bjbms.2018.2350

Authors

Jing Zhou Department of Nuclear Medicine, The Fuling Central Hospital of Chongqing, Chongqing, China
Gang Cheng Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
Hua Pang Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
Qian Liu Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
Ying Liu Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

DOI:

https://doi.org/10.17305/bjbms.2018.2350

Keywords:

Hypothyroidism, I-131, radioactive iodine, aorta, nitric oxide, NO, nitric oxide synthase, NOS, eNOS, nNOS, iNOS, inﬂammatory mediators, interleukin, IL-6, TNF-α

Abstract

Accumulating evidence has shown that hypothyroidism affects the cardiovascular system, significantly increasing the incidence of cardiovascular diseases. In the present study we investigated the effect of radioactive iodine (I-131)-induced hypothyroidism on several parameters of vascular function, such as nitric oxide (NO), total nitric oxide synthase (NOS) activity and expression of NOS isoforms, as well as on interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) as indicators of inflammation, in rats. A dose of 150 µCi of 131-I was determined as optimal for establishing the model of hypothyroidism in rats. After administration of 131-I, at the end of month 1, 2, and 4 (n = 3 for each time point), NO, IL-6, and TNF-α in the serum and total NOS activity in the aorta were determined in 150 µCi group, compared to controls. The mRNA and protein expression of endothelial, neuronal, and inducible NOS (eNOS, nNOS, and iNOS) in the rat aorta was also estimated, using quantitative reverse transcription polymerase chain reaction and Western blot, respectively. The levels of IL-6 and TNF-α increased in 150 µCi group; the results were significant at the end of month 2 and 4 for IL-6, and at all time points for TNF-α. The levels of NO decreased significantly at the end of month 2 and 4 in 150 µCi group. The total NOS activity increased significantly in 150 µCi group, at all three time points. Significant changes in the mRNA and protein expression of all three NOS isoforms were observed in 150 µCi group compared to controls. NO, IL-6, TNF-α levels and NOS activity and expression are altered in hypothyroid state, and the underlying mechanism should be further investigated.

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Author Biographies

Jing Zhou, Department of Nuclear Medicine, The Fuling Central Hospital of Chongqing, Chongqing, China

Department of Nuclear Medicine
Gang Cheng, Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

Department of Nuclear Medicine
Hua Pang, Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

Department of Nuclear Medicine
Qian Liu, Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

Department of Nuclear Medicine
Ying Liu, Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

Department of Nuclear Medicine

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