Different effects of sonoporation on cell morphology and viability

  • Ji-Zhen Zhang Department of Ultrasound In Medicine, Shanghai Jiao Tong University Affiliated 6th People’s Hospital, Shanghai Institute of Ultrasound in Medicine
  • Jasdeep K. Saggar Department of Medical Biophysics, University of Toronto
  • Zhao-Li Zhou Central Research Institute, Shanghai, Pharmaceuticals Holding Co., Ltd. Division of Antitumor Pharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
  • Bing Hu Department of Ultrasound In Medicine, Shanghai Jiao Tong University Affiliated 6th People’s Hospital, Shanghai Institute of Ultrasound in Medicine
Keywords: sonoporation, molecular delivery, drug delivery, ultrasound, low frequency ultrasound, microbubble contrast agents, cell morphology

Abstract

The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a cell impermeant dye). We investigated changes in cell morphology immediately after sonoporation under scanning electron microscope (SEM) and changes in cell viability immediately and 6 h after sonoporation under fluorescence microscope. It was shown that various levels of intracellular calcein uptake and changes in cell morphology can be caused immediately after sonoporation: smooth cell surface, pores in the membrane and irregular cell surface. Immediately after sonoporation, both groups of cells with high levels of calcein uptake and low levels of calcein uptake were viable; 6 h after sonoporation, group of cells with low levels of calcein uptake still remained viable, while group of cells with high levels of calcein uptake died. Sonoporation induces different effects on cell morphology, intracellular calcein uptake and cell viability.

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Different effects of sonoporation on cell morphology and viability
Published
2012-05-20
How to Cite
1.
Zhang J-Z, K. Saggar J, Zhou Z-L, Hu B. Different effects of sonoporation on cell morphology and viability. Bosn J of Basic Med Sci [Internet]. 2012May20 [cited 2020Sep.30];12(2):64-8. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2497
Section
Molecular Biology