Trefoil factor family peptides TFF1 and TFF3 in the nervous tissues of developing mouse embryo

Tatjana Belovari; Nikola Bijelić; Maja Tolušić Levak; Mirela Baus Lončar

doi:10.17305/bjbms.2015.251

Authors

Tatjana Belovari Department of Histology and Embryology, Faculty of Medicine, University of Osijek, Osijek
Nikola Bijelić Department of Histology and Embryology, Faculty of Medicine, University of Osijek, Osijek http://orcid.org/0000-0003-4136-820X
Maja Tolušić Levak Department of Histology and Embryology, Faculty of Medicine, University of Osijek, Osijek
Mirela Baus Lončar Department of Molecular Medicine, Institute Ruđer Bošković, Zagreb, Croatia

DOI:

https://doi.org/10.17305/bjbms.2015.251

Keywords:

trefoil factor, nervous system, embryonic development, mouse, immunohistochemistry

Abstract

Trefoil factor family peptides (TFF1, TFF2, and TFF3) are predominantly found in mucous epithelia of various organs. However, they have also been reported in the nervous tissue, particularly mouse, rat, porcine, and human brain. The aim of this research was to determine the presence of TFF1 and TFF3 in the nervous system of developing mouse embryo. Mouse embryos, at the stages E15 to E17 were isolated, fixed in 4% paraformaldehyde and embedded in paraffin blocks. Sagittal 6µm sections were made, processed for immunohistochemistry, and incubated with anti-TFF1 or anti-TFF3 primary polyclonal rabbit antibodies. Labeled streptavidin-biotin method was used for TFF detection. TFF1 and 3 were found in the cytoplasm of ganglion cell somata, while TFF3 staining was also visible in the cytoplasm of neurons in different areas and nuclei of brain and medulla oblongata. Neurons in the gray matter of spinal cord were also TFF1 and TFF3 positive, and signal for both peptides was found in the choroid plexus. TFF peptides might be involved in the complex processes of nervous system development and differentiation and brain plasticity.

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