Analysis of long non-coding RNA (lncRNA) expression in hepatitis B patients

Sunde Yılmaz Susluer; Cagla Kayabasi; Besra Ozmen Yelken; Aycan Asik; Didem Celik; Tugce Balci Okcanoglu; Suheyla Serin Senger; Cigir Biray Avci; Sukran Kose; Cumhur Gunduz

doi:10.17305/bjbms.2018.2800

Authors

Sunde Yılmaz Susluer Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey
Cagla Kayabasi Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey
Besra Ozmen Yelken Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey
Aycan Asik Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey
Didem Celik Izmir Tepecik Education and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Izmir, Turkey
Tugce Balci Okcanoglu Vocational School of Health Sciences, Near East University, Nicosia, Turkish Republic of Northern Cyprus (TRNC)
Suheyla Serin Senger Izmir Tepecik Education and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Izmir, Turkey
Cigir Biray Avci Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey
Sukran Kose Izmir Tepecik Education and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Izmir, Turkey
Cumhur Gunduz Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

DOI:

https://doi.org/10.17305/bjbms.2018.2800

Keywords:

HBV, lncRNA, chronic hepatitis B, inactive HBsAg carrier, resolved hepatitis B

Abstract

Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes, including epigenetic regulation, cell-cycle control, and transcriptional/translational regulation of gene expression. Differential expression of lncRNAs and disruption of the regulatory processes are recognized as critical steps in cancer development. The role of lncRNAs in hepatitis B virus (HBV) infection is not well understood. Here we analyzed the expression of 135 lncRNAs in plasma samples of 82 HBV patients (classified as chronic patients, inactive carriers, or resolved patients) at diagnosis and at 12 months of treatment in relation to control group (81 healthy volunteers). We also investigated the effect of small interfering RNA (siRNA)-mediated silencing of lincRNA-SFMBT2 on HBV-positive human liver cancer cell line. lncRNA expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Chemically synthesized siRNAs were transfected into the cell lines using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). HBV DNA and HBsAg and HBeAg were detected in transfected cultures by real-time PCR and ELISA, respectively, using commercial kits. We observed changes in lncRNA expression in all three HBV groups, compared to control group. Most notably, the expression of anti-NOS2A, lincRNA-SFMBT2, and Zfhx2as was significantly increased and expression of Y5 lncRNA was decreased in chronic HBV patients. A decreased Y5 expression and increased lincRNA-SFMBT2 expression were observed in inactive HBsAg carriers. The expression of HOTTIP, MEG9, and PCAT-32 was increased in resolved HBV patients, and no significant change in the expression of Y5 was observed, compared to control group. siRNA-mediated inhibition of lincRNA-SFMBT2 decreased the level of HBV DNA in human liver cancer cells. Further research is needed to confirm the prognostic as well as therapeutic role of these lncRNAs in HBV patients.

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Author Biographies

Sunde Yılmaz Susluer, Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

Department of Medical Biology
Cagla Kayabasi, Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

Department of Medical Biology
Besra Ozmen Yelken, Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

Department of Medical Biology
Aycan Asik, Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

Department of Medical Biology
Cigir Biray Avci, Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

Department of Medical Biology
Cumhur Gunduz, Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey

Department of Medical Biology

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