In Vitro Examination of Ontogenesis of Developing Neuronal Cells in Vagal Nuclei in Medulla Oblongata in Newborns

Hilmi Islami; Ragip Shabani; Sadi Bexheti; Ibrahim Behluli; Aziz Šukalo; Denis Raka; Rozafa Koliqi; Naim Haliti; Hilmi Dauti; Shaip Krasniqi; Mentor Disha

doi:10.17305/bjbms.2008.2904

Authors

Hilmi Islami Department of Pharmacology, Faculty of Medicine, University of Prishtina, Clinical Centre
Ragip Shabani Department of Pathology, Faculty of Medicine, University of Prishtina, Clinical Centre
Sadi Bexheti Department of Anatomy, Faculty of Medicine, University of Prishtina, Clinical Centre
Ibrahim Behluli Department of Anatomy, Faculty of Medicine, University of Prishtina, Clinical Centre
Aziz Šukalo Drugs factory-Bosnalijek -Sarajevo, Bosnalijek dd
Denis Raka Department of Pharmacy, Faculty of Medicine, University of Prishtina, Clinical Centre
Rozafa Koliqi Department of Pharmacy, Faculty of Medicine, University of Prishtina, Clinical Centre
Naim Haliti Department of Forensic Medicine, Faculty of Medicine, University of Prishtina, Clinical Centre
Hilmi Dauti Department of Anatomy, Faculty of Medicine, University of Prishtina, Clinical Centre
Shaip Krasniqi Department of Pharmacology, Faculty of Medicine, University of Prishtina, Clinical Centre
Mentor Disha Department of Pharmacology, Faculty of Medicine, University of Prishtina, Clinical Centre

DOI:

https://doi.org/10.17305/bjbms.2008.2904

Keywords:

medulla oblongata, DMNV, NTS

Abstract

The development of neuron cells in vagal nerve nuclei in medulla oblongata was studied in vitro in live newborns and stillborns from different cases. Morphological changes were studied in respiratory nuclei of dorsal motor centre (DMNV) and nucleus tractus solitarius (NTS) in medulla oblongata. The material from medulla oblongata was fixated in 10μ buffered formalin solution. Fixated material was cut in series of 10μ thickness, with starting point from obex in ± 4 mm thickness. Special histochemical and histoenzymatic methods for central nervous system were used: cresyl echt violet coloring, tolyidin blue, Sevier-Munger modification and Grimelius coloring. In immature newborns (abortions and immature) in dorsal motor nucleus of the vagus (DMNV) population stages S1, S2, S3 are dominant. In neuron population in vagal sensory nuclei (NTS) stages S1, S2 are dominant. In more advanced stages of development of newborns (premature), in DMNV stages S3 and S4 are seen and in NTS stages S2 and S3 are dominant. In mature phase of newborns (maturity) in vagal nucleus DMNV stages S5 and S6 are dominant, while in sensory nucleus NTS stages S4 and S5 are dominant. These data suggest that neuron population in dorsal motor nucleus of the vagus (DMNV) are more advanced in neuronal maturity in comparison with sensory neuron population of vagal sensory nucleus NTS. This occurrence shows that phylogenetic development of motor complex is more advanced than the sensory one, which is expected to take new information’s from the extra uterine life after birth (extra uterine vagal phenotype)