Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa

Isar Dejban Golpasha; Seyed Fazlollah Mousavi; Parviz Owlia; Seyed Davar Siadat; Shiva Irani

doi:10.17305/bjbms.2015.292

Authors

Isar Dejban Golpasha Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
Seyed Fazlollah Mousavi Microbiology Research Center and Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Parviz Owlia Molecular Microbiology Research Center, Shahed University, Tehran, Iran
Seyed Davar Siadat Microbiology Research Center and Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Shiva Irani Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran

DOI:

https://doi.org/10.17305/bjbms.2015.292

Keywords:

3-oxo-C12-HSL, Homoserine lactone (HSL), Immunization, PcrV, Pseudomonas aeruginosa, Vaccine

Abstract

Quorum Sensing and type III secretion system play an important role in the virulence of Pseudomonas (P.) aeruginosa in burn wound infections. We aimed to explore the feasibility of using 3-oxo-C₁₂-HSL-r-PcrV conjugate as a candidate vaccine against P. aeruginosa caused infections. 3-oxo-C₁₂-HSL-r-PcrV conjugate was prepared and used for immunization of mice (10 μg, subcutaneous, three times, at 2-week intervals). Mice were divided into five groups: I: PcrV; II: 3-oxo-C₁₂-HSL-r-PcrV (10 μg); III: 3-oxo-C₁₂-HSL-r-PcrV (20 μg); IV: 3-oxo-C₁₂-HSL; and V: PBS receiving groups. After each shot of immunization, total and isotype antibody responses against corresponding antigen were measured to determine the immunization efficacy. One month after the last immunization, all groups were burned and challenged subeschar with P. aeruginosa PAO1. Survival rate and bacterial quantity in the skin and internal organs (liver and spleen) were evaluated 25-hr after burn infection. Immunization with 3-oxo-C₁₂-HSL-r-PcrV significantly increased total IgG and specific subclass antibodies (IgG₁, IgG₂a, IgG₂b, and IgM) in the serum of the groups II and III compared to the control group (p<0.001). While all the control mice (PBS injected group) died within 2 days after bacterial challenge, 64% of the group I, 78% of group II, and 86% of group III, survived within 14 days after challenge. Interestingly, bacterial burden in the liver and spleen of 3-oxo-C₁₂-HSL-r-PcrV injected group (III) was significantly lower than the control group (p<0.001). The present study proposed two-component vaccine to inhibit Pseudomonas infections in burned mouse.

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Author Biography

Isar Dejban Golpasha, Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran

Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran

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