Daily Fluctuation of Cortisol in the Saliva and Serum of Healthy Persons

  • Nevzeta Ljubijankić Faculty of Science, University of Sarajevo
  • Ranka Popović-Javorić Faculty of Science, University of Sarajevo
  • Sabiha Šćeta Institute of Clinics Biochemistry, University of Sarajevo Clinical Centre
  • Aida Šapčanin Institute of Clinics Biochemistry, University of Sarajevo Clinical Centre
  • Ismet Tahirović Faculty of Science, University of Sarajevo
  • Emin Sofić Faculty of Science, University of Sarajevo
Keywords: cortisol, serum, saliva

Abstract

The objective of this study was to evaluate cortisol in the saliva and serum of healthy persons and its daily fluctuations by using immunochemical method on a autoanalyzer. Biological samples: Serum from 14 healthy persons and saliva from 18 healthy persons were taken two times at 8 a.m. and at 4 p.m. Immunochemical assay: The principle of this method is the competitive binding of cortisol present in the analyzed sample and cortisol marked with peroxides on binding parts with specific antibodies. Statistical analysis: Student t-test. Cortisol in saliva in the morning: 21,2 ± 16,2 nmol/l and in the afternoon 12,7 ± 8,1 nmol/l. Cortisol in serum in the morning: 459, 6 ± 235,2 nmol/l, and in the afternoon 340,5 ± 207,5 nmol/l. The concentrations of cortisol in saliva are lower than in serum. Cortisol in the serum in the morning is about twenty times higher than cortisol in the saliva at the same time. Cortisol in the serum at afternoon is about twenty-seven times higher than cortisol in the saliva. Individual variabilities of cortisol in the saliva and serum were found during the day.

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Daily Fluctuation of Cortisol in the Saliva and Serum of Healthy Persons
Published
2008-05-20
How to Cite
1.
Ljubijankić N, Popović-Javorić R, Šćeta S, Šapčanin A, Tahirović I, Sofić E. Daily Fluctuation of Cortisol in the Saliva and Serum of Healthy Persons. Bosn J of Basic Med Sci [Internet]. 2008May20 [cited 2020Oct.25];8(2):110-5. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2962
Section
Biochemistry