Effect of Lactate on Insulin Action in Rats

  • Muhidin Hamamdžić Department of Physiology, Faculty of Veterinary Medicine, University of Sarajevo
  • Boris Hrabač Department of Sport Physiology, Faculty of Sports, University of Sarajevo
  • Amer Alić Department of Pathology, Faculty of Veterinary Medicine, University of Sarajevo
  • Eva Pašić-Juhas Department of Physiology, Faculty of Veterinary Medicine, University of Sarajevo
  • Aida Hodžić Department of Physiology, Faculty of Veterinary Medicine, University of Sarajevo
Keywords: lactate, insulin, glucose uptake, rats

Abstract

The aim of the study was to explore the effect of lactate on insulin-stimulated glucose uptake in rats. Thirty Wistar rats, weighing 250 - 300 g. were arbitrarily divided into one of three groups (n =10): insulin (1 IU/kg) treated group, lactate (80 mg/kg), and insulin plus lactate treated groups. Blood glucose levels were measured in venous samples collected from the tail vein over 3 hour period after insulin or/and lactate administration in 30-minute intervals.

To estimate the influence of lactate on insulin blood level, a total of 20 rats were divided into 4 groups (n = 5): saline, insulin, lactate, and insulin plus lactate treated group, respectively.

Sixty minutes after the appropriate application of the same doses of insulin, lactate, and lactate plus insulin, as in the previous part of the experiment, plasma insulin and blood glucose levels were determined in blood samples drawn from the abdominal aorta. Lactate in combination with insulin, in comparison to insulin application alone, caused a dramatic increase in plasma insulin level (p<0,001) and more profound hypoglicaemia (p<0,001). The results of this investigation indicate that lactate application significantly increases the rate of glucose uptake from peripheral blood caused by exogenous insulin action. The possible involvement of lactate in the mechanism of enhanced glucose uptake due to insulin action after physical exercise is discussed.

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Effect of Lactate on Insulin Action in Rats
Published
2008-05-20
How to Cite
1.
Hamamdžić M, Hrabač B, Alić A, Pašić-Juhas E, Hodžić A. Effect of Lactate on Insulin Action in Rats. Bosn J of Basic Med Sci [Internet]. 2008May20 [cited 2020Sep.30];8(2):131-4. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2967
Section
Biochemistry