Correlation of Autoantibodies Presence Detected By IFA-anti-dsDNA, IFA-AMA and Immunoblotting with Corresponding Data in Clinical Management of Autoimmune Diseases

Đemo Subašić; Jasenko Karamehić; Farid Ljuca; Faris Gavrankapetanović; Marina Delić-Šarac; Izet Eminović; Dragan Kovačević

doi:10.17305/bjbms.2008.3004

Authors

Đemo Subašić Institute of Clinical Immunology - University of Sarajevo Clinics Centre
Jasenko Karamehić Institute of Clinical Immunology - University of Sarajevo Clinics Centre
Farid Ljuca Department of Physiology, Faculty of Medicine, University of Tuzla
Faris Gavrankapetanović Institute of Clinical Immunology - University of Sarajevo Clinics Centre
Marina Delić-Šarac Institute of Clinical Immunology - University of Sarajevo Clinics Centre
Izet Eminović Faculty of Science, University of Tuzla
Dragan Kovačević Institute of Clinical Immunology - University of Sarajevo Clinics Centre

DOI:

https://doi.org/10.17305/bjbms.2008.3004

Keywords:

SLE, AIH, PBC, IFA, AMA, anti-dsDNA, M-2, LKM-1, LC-1, SLA/LP

Abstract

Diagnosis and management of patients with SLE (Systemic Lupus Eritematosus), autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), involves specific diagnostic tests, such as IFA-AMA, IFA anti-dsDNA and immunoblotting for the detection of autoantibodies for specific autoantigens (mitochondria, dsDNA, M2, LKM-1, LC-1, SLA/LP). We established specific correlation between the detected autoantibodies and corresponding clinical findings.

The total of 813 serum specimens were probed with IFA-anti-dsDNA, 98 of which tested positive. We also performed dilution analysis to the end point for all the positive specimens. Numerous specimens were tested by IFA, AMA and immunoblotting.