DOI: http://dx.doi.org/10.17305/bjbms.2018.3399 | Full Text: [Provisional PDF]

Overexpression of miR-361-5p in triple-negative breast cancer (TNBC) inhibits migration and invasion by targeting RQCD1 and inhibiting the EGFR/ PI3K/Akt pathway

Jianjun Han, Jingjing Yu, Yu'na Dai, Jumei Li, Meiyan Guo, Jingzhen Song, Xuefeng Zhou

Abstract


Triple-negative breast cancer (TNBC) is the leading cause of cancer-related death in women. Previous studies indicated that miR-361-5p was downregulated in breast cancer, however, the exact effect of miR-361-5p on TNBC requires further investigation. In the present study, we investigated whether miR-361-5p can act as a tumor suppressor by targeting required for cell differentiation 1 homolog (RQCD1) and inhibiting epidermal growth factor receptor (EGFR)/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway in TNBC. The expression of miR-361-5p and RQCD1 was determined by quantitative reverse transcription PCR (qRT-PCR) and/or western blot in TNBC and the adjacent tissues. miR-361-5p mimics were constructed and transfected to TNBC cell line MDA-MB-231. Expression of miR-361-5p and mRNA and protein expression of RQCD1, PI3K, Akt, EGFR and matrix metallopeptidase 9 (MMP-9) in MDA-MB-231 were measured by qRT-PCR and/or western blot after transfection. Cell viability was determined by CCK-8 assay. Cell migration and invasion ability were evaluated by scratch and transwell assay respectively. Confirmation of miR-361-5p target was determined by bioinformatics analysis and luciferase reporter assay. Downregulated miR-361-5p and upregulated RQCD1 were observed in tumor tissues. Expression of EGFR, PI3K, Akt and MMP-9 was inhibited in cells treated with miR-361-5p mimics. Transfection of miR-361-5p mimics also inhibited the phosphorylation of EGFR, PI3K, and Akt. Suppressed cell viability, migration, and invasion was found in miR-361-5p mimics groups. Luciferase reporter assay showed that RQCD1 was the target of miR-361-5p. These results indicated that overexpression of miR-361-5p might act as a suppressor in TNBC by targeting RQCD1 to inhibit the EGFR/PI3K/Akt signaling pathway.


Keywords


miR-361-5p; RQCD1; EGFR/PI3K/Akt signaling pathway; cell migration and invasion; triple-negative breast cancer; TNBC

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