Effects of epinephrine on heart rate variability and cytokines in a rat sepsis model

Authors

  • Yun-Te Chang Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Kaohsiung City; Department of Nursing, Yuh-Ing Junior College of Health Care and Management, Kaohsiung City; Department of Physical Therapy, Shu-Zen Junior College of Medicine and Management, Kaohsiung City; School of Medicine, National Yang-Ming University, Taipei City, Taiwan, Republic of China
  • Wei-Chun Huang Cardiovascular Medical Center, Kaohsiung Veterans General Hospital, Kaohsiung City, Taiwan, Republic of China
  • Chin-Chang Cheng Cardiovascular Medical Center, Kaohsiung Veterans General Hospital, Kaohsiung City, Taiwan, Republic of China
  • Meng-Wei Ke Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung City, Taiwan, Republic of China
  • Jung-Shun Tsai Department of Nursing, Yuh-Ing Junior College of Health Care and Management, Kaohsiung City, Taiwan, Republic of China
  • Yao-Min Hung Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Kaohsiung City, Taiwan, Republic of China
  • Neng-Chyan Huang Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Kaohsiung City, Taiwan, Republic of China
  • Mu-Shun Huang School of Medicine, National Yang-Ming University, Taipei City, Taiwan, Republic of China
  • Shue-Ren Wann Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Kaohsiung City, Taiwan, Republic of China

DOI:

https://doi.org/10.17305/bjbms.2018.3565

Keywords:

Sepsis, heart rate variability, HRV, epinephrine, EPI, inflammation, β-blocker, rats

Abstract

Catecholamines have both anti-inflammatory and vasoactive properties. A decreased cardiac response to catecholamines has been associated with a high risk of death in sepsis and septic shock. The aim of this study was to investigate the effects of epinephrine (EPI) on heart rate variability (HRV) and autonomic balance, as well as cytokine levels, in a rat sepsis model. Thirty-six male Sprague-Dawley rats were assigned to 4 experimental groups and 2 control groups of 6 rats each. The rats in the experimental groups were inoculated with a lipopolysaccharide (LPS, endotoxin) to establish a sepsis model. Group A received only LPS; group B received LPS, antecedent EPI and the nonselective β-blocker propranolol; group C received LPS and antecedent EPI; and group D received LPS, antecedent EPI and the selective β1-blocker esmolol. One control group received EPI and the other received saline placebo. Heart rate variability (HRV) was analyzed and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) levels were measured. Measurements were carried out at baseline, at 0 hour after EPI infusion, and at 0.5, 2, and 4 hours after LPS inoculation. There were significant differences in HRV and cytokine levels between the groups, indicating that LPS infusion caused autonomic imbalance. Antecedent EPI significantly decreased the level of TNF-α in group C compared with group A in which TNF-α level peaked at 2 hours and then declined. Propranolol (group B) but not esmolol (group D) administration resulted in elevated TNF-α levels, comparable to those observed in group A. In conclusion, antecedent administration of EPI in a rat sepsis model inhibits the production of TNF-α possibly via the β2-adrenoceptor.

Author Biography

  • Yun-Te Chang, Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Kaohsiung City; Department of Nursing, Yuh-Ing Junior College of Health Care and Management, Kaohsiung City; Department of Physical Therapy, Shu-Zen Junior College of Medicine and Management, Kaohsiung City; School of Medicine, National Yang-Ming University, Taipei City, Taiwan, Republic of China

     

     

Effects of epinephrine on heart rate variability and cytokines in a rat sepsis model

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Published

05-02-2020

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Translational and Clinical Research

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How to Cite

1.
Effects of epinephrine on heart rate variability and cytokines in a rat sepsis model. Biomol Biomed [Internet]. 2020 Feb. 5 [cited 2024 Mar. 3];20(1):88-9. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/3565