Enhancement of bone consolidation using high-frequency pulsed electromagnetic fields (HF-PEMFs): An experimental study on rats

Authors

  • Daniel Oltean-Dan “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Gabriela Bombonica Dogaru “Iuliu Hatieganu” University of Medicine and Pharmacy; Clinical Rehabilitation Hospital of Cluj-Napoca, Cluj-Napoca, Romania
  • Dragos Apostu “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Alexandru Mester “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Horea Rares Ciprian Benea “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Mihai Gheorghe Paiusan “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Catalin Ovidiu Popa Technical University, Cluj-Napoca, Romania
  • Elena Mihaela Jianu “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Gyorgy Istvan Bodizs Clinical Rehabilitation Hospital of Cluj-Napoca, Cluj-Napoca, Romania
  • Cristian Berce “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Alina Mihaela Toader “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Gheorghe Tomoaia “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca; Academy of Romanian Scientists, Bucharest, Romania

DOI:

https://doi.org/10.17305/bjbms.2019.3854

Keywords:

fracture healing, electromagnetic fields, titanium 6-aluminum-4-vanadium, osteogenic markers, bone remodelling, bone consolidation, in vivo rat model, high-frequency pulsed electromagnetic fields, PEMFs

Abstract

In vitro studies showed that high-frequency pulsed electromagnetic fields (HF-PEMFs) increase the activity/expression of early and late osteogenic markers and enhance bone mineralization. The main aim of this study was to investigate the in vivo effects of HF-PEMFs on fracture healing using a rat model. A femur fracture was established by surgery in 20 male Wistar rats. Titanium nails were implanted to reduce and stabilize the fracture. After surgery, 20 rats were equally divided into untreated control and treated group (from the first postoperative day HF-PEMFs at 400 pulses/sec [pps] were applied for 10 minutes/day, for two weeks). Quantitative and qualitative assessment of bone formation was made at two and eight weeks following surgery and included morphological and histological analysis, serological analysis by ELISA, micro-computed tomography (micro-CT), and three-point bending test. At two weeks in HF-PEMF group, soft callus was at a more advanced fibrocartilaginous stage and the bone volume/total tissue volume (BV/TV) ratio in the callus area was significantly higher compared to control group (p = 0.047). Serum concentration of alkaline phosphatase (ALP) and osteocalcin (OC) was significantly higher in HF-PEMF group (ALP p = 0.026, OC p = 0.006) as well as the mechanical strength of femurs (p = 0.03). At eight weeks, femurs from HF-PEMF group had a completely formed woven bone with dense trabeculae, active bone marrow, and had a significantly higher BV/TV ratio compared to control (p = 0.01). HF-PEMFs applied from the first postoperative day, 10 minutes/day for two weeks, enhance bone consolidation in rats, especially in the early phase of fracture healing.

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Author Biographies

  • Daniel Oltean-Dan, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Orthopedics and Traumatology

  • Gabriela Bombonica Dogaru, “Iuliu Hatieganu” University of Medicine and Pharmacy; Clinical Rehabilitation Hospital of Cluj-Napoca, Cluj-Napoca, Romania

    Department of Medical Rehabilitation

  • Dragos Apostu, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Orthopedics and Traumatology

  • Alexandru Mester, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Oral Rehabilitation

  • Horea Rares Ciprian Benea, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Orthopedics and Traumatology

  • Mihai Gheorghe Paiusan, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Orthopedics and Traumatology

  • Catalin Ovidiu Popa, Technical University, Cluj-Napoca, Romania

    Department of Materials Science and Engineering

  • Elena Mihaela Jianu, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Histology

  • Alina Mihaela Toader, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Department of Physiology

  • Gheorghe Tomoaia, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca; Academy of Romanian Scientists, Bucharest, Romania

    Department of Orthopedics and Traumatology

Enhancement of bone consolidation using high-frequency pulsed electromagnetic fields (HF-PEMFs): An experimental study on rats

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Published

20-05-2019

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New and Emerging Methods

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How to Cite

1.
Enhancement of bone consolidation using high-frequency pulsed electromagnetic fields (HF-PEMFs): An experimental study on rats. Biomol Biomed [Internet]. 2019 May 20 [cited 2024 Jul. 19];19(2):201-9. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/3854