Role of S180L polymorphism in etiology of malaria caused by Plasmodium falciparum in a small group of Pakistani population

Syed Kashif Nawaz; Bisma Ahmed; Najma Arshad; Asima Rani; Hamadia Rasool; Muhammad Arshad

doi:10.17305/bjbms.2015.413

Authors

Syed Kashif Nawaz Department of Zoology, University of Sargodha, Sargodha- Pakistan
Bisma Ahmed Department of Zoology, University of Sargodha, Sargodha- Pakistan
Najma Arshad Department of Zoology, University of the Punjab, Lahore- Pakistan
Asima Rani Department of Zoology, University of Sargodha, Sargodha- Pakistan
Hamadia Rasool Department of Zoology, University of Sargodha, Sargodha- Pakistan
Muhammad Arshad Department of Zoology, University of Sargodha, Sargodha- Pakistan

DOI:

https://doi.org/10.17305/bjbms.2015.413

Keywords:

S180L, polymorphism, malaria, Plasmodium falciparum, MAL/TIRAP

Abstract

The aim of our study was to investigate the role of S180L polymorphism in modulation of acquisition of malaria caused by Plasmodium falciparum in a small group of Pakistani population. A total of 133 individuals including 60 controls and 73 patients of malaria, caused by Plasmodium falciparum, were genotyped using allele-specific PCR. Ninety-two samples successfully demonstrated the PCR amplification results, while forty-one samples could not be genotyped due to failure in PCR amplification. The allele frequency for S180L polymorphism was deviant from Hardy-Weinberg equilibrium (HWE) of the population under observation. Association was found between the observed polymorphism and the occurrence of malaria caused by Plasmodium falciparum (p = 0.01). Chances of malaria caused by Plasmodium falciparum were low in CC genotype carriers in comparison to other genotypes (Odds ratio: 0.3016; 95% CI: 0.124-0.729). The present findings suggest that S180L polymorphism is important in modulating the probability of acquisition of malaria caused by Plasmodium falciparum in Pakistani population. The CC genotype plays a protective role in local population against this type of malaria.

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Author Biographies

Syed Kashif Nawaz, Department of Zoology, University of Sargodha, Sargodha- Pakistan

Assistant Professor,
Department of Zoology,
University of Sargodha,
Sargodha- Pakistan
Bisma Ahmed, Department of Zoology, University of Sargodha, Sargodha- Pakistan

M.Phil student,Department of Zoology,
University of Sargodha,
Sargodha- Pakistan
Muhammad Arshad, Department of Zoology, University of Sargodha, Sargodha- Pakistan

Professor,
Department of Zoology,
University of Sargodha,
Sargodha- Pakistan

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