Expression of the anti-Mullerian hormone, kisspeptin 1, and kisspeptin 1 receptor in polycystic ovary syndrome and controlled ovarian stimulation rat models

Ali  Risvanli; Halis  Ocal; Necati Timurkaan; Pinar Ipek; Ibrahim Seker; Burak  Karabulut

doi:10.17305/bjbms.2019.4281

Authors

Ali Risvanli Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey https://orcid.org/0000-0001-5653-0025
Halis Ocal Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey
Necati Timurkaan Department of Pathology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey https://orcid.org/0000-0001-5567-991X
Pinar Ipek Yesilhisar Health Vocational School, Univerity of Erciyes, Kayseri, Turkey
Ibrahim Seker Department of Zootechny, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey
Burak Karabulut Department of Pathology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey

DOI:

https://doi.org/10.17305/bjbms.2019.4281

Keywords:

Anti-Mullerian hormone, kisspeptin, ovarian stimulation, polycystic ovary syndrome, COS, PCOS, KISS-1, KISS1r, GPR54 receptor

Abstract

Polycystic ovary syndrome represents a significant cause of female infertility. The aim of this study was to investigate the expression of anti-Mullerian hormone (AMH), kisspeptin 1 (KISS-1), and kisspeptin 1 receptor (KISS1r) in rat models of polycystic ovary syndrome (PCOS) and controlled ovarian stimulation (COS). For this purpose, 28 rats were assigned into four groups. Estrus and Diestrus groups consisted of rats in estrus and diestrus phases, respectively, while COS and PCOS groups consisted of rats with induced COS and PCOS, respectively. The serum AMH, KISS-1, and estradiol levels, and ovarian KISS1r levels were analyzed by enzyme-linked immunosorbent assay. Furthermore, histopathological analysis of the ovary tissue was done and ovarian KISS-1 expression was determined by immunohistochemical assay. The results revealed that ovarian KISS1r levels were higher in the Estrus (1271.43±51.98 pg/mL) and COS (1191.43±85.67 pg/mL) groups, compared to Diestrus and PCOS groups. The highest level of AMH was found in the Estrus group (16.91±2.12 ng/mL). The results indicate that AMH had no effect on the development of COS and PCOS, while KISS-1 was found to affect the development of COS in rats.