Knockdown of TPT1-AS1 inhibits cell proliferation, cell cycle G1/S transition, and epithelial–mesenchymal transition in gastric cancer

  • Jun Tang Department of General Surgery, The Center Hospital of Ezhou, Ezhou, China
  • Fei Huang Department of Medical Laboratory, The Center Hospital of Ezhou, Ezhou, China
  • Hui Wang Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
  • Feng Cheng Department of Medical Laboratory, The Center Hospital of Ezhou, Ezhou, China
  • Yaping Pi Department of General Surgery, The Center Hospital of Ezhou, Ezhou, China
  • Juanjuan Zhao Department of Pathology, The Center Hospital of Ezhou, Ezhou, China
  • Zhihong Li Department of General Surgery, The Center Hospital of Ezhou, Ezhou, China https://orcid.org/0000-0002-5846-847X
Keywords: Gastric cancer, TPT1-AS1, prognosis, G1/S transition, epithelial– mesenchymal transition, EMT

Abstract

Long non-coding RNAs are considered to be critical regulators of tumor progression. Tumor protein translationally controlled 1 antisense RNA 1 (TPT1-AS1) was shown to have an oncogenic role in cervical and ovarian cancer. The clinical significance and biological function of TPT1-AS1 in gastric cancer (GC) are not clear. In this study, we analyzed the expression of TPT1-AS1 in GC tissues and cell lines and performed functional and mechanistic analysis of TPT1-AS1 effects on GC cell proliferation, migration, and invasion. TPT1-AS1 expression was determined in 76 pairs of GC tissues vs. matched adjacent normal tissues and in four GC cell lines (SGC-7901, AGS, BGC-823, and MGC-803) vs. GES-1 cell line by quantitative reverse transcription PCR. SGC-7901 and MGC-803 cells were transfected with small interfering RNA or scrambled negative control, and cell proliferation, colony formation, migration, invasion and cell cycle assays were performed. The expression of proteins involved in cell cycle progression and epithelial–mesenchymal transition was analyzed by Western blot. TPT1-AS1 expression was significantly higher in GC tissues and cell lines compared to controls. The overexpression of TPT1-AS1 was significantly correlated with TNM stage and lymph node metastasis, and it was associated with worse prognosis of GC patients according to the Kaplan–Meier survival analysis and Cox proportional hazard regression analysis. The knockdown of TPT1-AS1 significantly inhibited proliferation, cell cycle G1/S transition, migration, and invasion of SGC-7901 and MGC-803 cells. Moreover, TPT1-AS1 knockdown downregulated the expression of CDK4, cyclin D1, and vimentin and upregulated the expression of p21 and E-cadherin. Our findings suggest that TPT1-AS1 may be a promising therapeutic target in GC.

Downloads

Download data is not yet available.
Knockdown of TPT1-AS1 inhibits cell proliferation, cell cycle G1/S transition, and epithelial–mesenchymal transition in gastric cancer
Published
2020-02-28
How to Cite
1.
Tang J, Huang F, Wang H, Cheng F, Pi Y, Zhao J, Li Z. Knockdown of TPT1-AS1 inhibits cell proliferation, cell cycle G1/S transition, and epithelial–mesenchymal transition in gastric cancer. Bosn J of Basic Med Sci [Internet]. 2020Feb.28 [cited 2020Apr.1];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/4470
Section
Molecular Biology