Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity

  • Qingli Wang Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China
  • Haiquan Ai Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China
  • Xia Li Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China
  • Haiqing Tian Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China
  • Bingxue Ning Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China
  • Meng Zhang Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China
  • Xiaolin La Department of Reproductive Medicine Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, XinJiang, China https://orcid.org/0000-0001-9089-229X
Keywords: miRNA-145, hydrosalpinx, infertility, inflammation, endometrial cells

Abstract

MiR-145 is reported to facilitate inflammation and is also associated with unsuccessful embryonic implantation. Whether miR-145 mediates inflammatory response underlying hydrosalpinx-induced defective endometrial receptivity (ER) remains unclear, and this study attempted to clarify this point. Endometrium samples were collected from hydrosalpinx patients (case, n = 243) and patients with tubal patency/obstruction (control, n = 187). The peripheral blood samples of cases and controls were collected to determine the genotypes of miR-145 SNPs. The value of miR-145 expression in the diagnosis and prognostic estimation of hydrosalpinx was assessed using ROC curve and regression analysis, respectively. Lipopolysaccharide (LPS) cell model was established with endometrial cells, and cells were transfected with miR-145 mimic, inhibitor, or negative control. MiR-145 and cytokine levels were quantified by quantitative reverse transcription PCR or western blot. MiR-145 expression was significantly higher in hydrosalpinx compared to control group, and high miR-145 expression was significantly associated with moderate/severe tube lesion, high pulsatility index (>1.06), and high resistance index (>0.61) in hydrosalpinx patients. ROC curve analysis indicated that monitoring miR-145 expression may be useful for the diagnosis of hydrosalpinx (AUC = 0.704). A alleles of rs41291957 (G>A) and rs353292 (G>A) were significantly associated with an increased risk of hydrosalpinx compared to G allele (p < 0.05), yet the mutant allele of rs353291 (A>G) and rs4705343 (T>C) significantly reduced susceptibility to hydrosalpinx compared to the wild type allele. Treatments with miR-145 mimic and LPS in endometrial cells significantly increased the levels of transforming growth factor-β1, tumor necrosis factor -α, interleukin (IL)-6, and IL-8 compared to negative control, while treatment with miR-145 inhibitor decreased the cytokine levels. In conclusion, abnormally expressed miR-145 may be involved in hydrosalpinx-induced ER defects by regulating the inflammatory response.

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Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity
Published
2020-04-01
How to Cite
1.
Wang Q, Ai H, Li X, Tian H, Ning B, Zhang M, La X. Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity. Bosn J of Basic Med Sci [Internet]. 2020Apr.1 [cited 2020Aug.7];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/4538
Section
Translational and Clinical Research

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