Development and validation of a ferroptosis-related lncRNAs prognosis signature in colon cancer

Authors

  • Hua-jun Cai Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Zhi-cheng Zhuang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China https://orcid.org/0000-0003-4376-5049
  • Yong Wu Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Yi-yi Zhang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Xing Liu Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Jin-fu Zhuang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Yuan-feng Yang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Yuan Gao Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Bin Chen Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Guo-xian Guan Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

DOI:

https://doi.org/10.17305/bjbms.2020.5617

Keywords:

TCGA, colon adenocarcinoma, ferroptosis, long non-coding RNA, prognosis signature

Abstract

Ferroptosis is a form of iron-dependent programmed cell death. Regulation of ferroptosis in tumor cells is a novel treatment modality. The present study aimed to investigate ferroptosis-related long non-coding RNAs (lncRNAs) and construct a prognostic model for colon adenocarcinoma (COAD). RNA- sequencing data and ferroptosis-related genes were obtained from The Cancer Genome Atlas database and FerrDb database. COAD patients were randomly assigned to training- and validation groups. The Least Absolute Shrinkage and Selection Operator regression and Cox regression model were used to determine and develop a predictive model. The model was corroborated using the validation group and the entire group. In total, 259 ferroptosis-related genes and 905 ferroptosis-related LncRNAs were obtained. Cox model revealed and constructed seven ferroptosis-related LncRNAs signature (LINC01503, AC004687.1, AC010973.2, AP001189.3, ARRDC1-AS1, OIP5-AS1, and NCK1-DT). Patients were assigned into two groups according to the median risk score. Kaplan–Meier survival curves showed that overall survival between high- and low-risk groups was statistically significant (P<0.01). Cox multivariate analysis seven ferroptosis-related LncRNAs signature was an independent risk factor for COAD outcomes (P<0.05). The relationship between seven ferroptosis-related LncRNAs and clinicopathological features was also examined. The principal component analysis showed a difference between high- and low-risk groups intuitively. With the aid of gene set enrichment analysis, the underlying mechanisms of seven ferroptosis-related LncRNAs were uncovered, including the MAPK signaling pathway, mTOR signaling pathway, and glutathione metabolism pathway. Finally, we established and validated seven ferroptosis-related lncRNAs signature for COAD patients to predict survival. These results may provide meaningful targets for future study.

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Author Biographies

  • Hua-jun Cai, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Zhi-cheng Zhuang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Yong Wu, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Yi-yi Zhang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Xing Liu, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Jin-fu Zhuang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Yuan-feng Yang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Yuan Gao, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

  • Bin Chen, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

    Department of Colorectal Surgery

Development and validation of a ferroptosis-related lncRNAs prognosis signature in colon cancer

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Published

01-10-2021

Issue

Section

Molecular Biology

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How to Cite

1.
Development and validation of a ferroptosis-related lncRNAs prognosis signature in colon cancer. Biomol Biomed [Internet]. 2021 Oct. 1 [cited 2024 May 19];21(5):569-76. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/5617

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