Development and validation of a ferroptosis-related lncRNAs prognosis signature in colon cancer

  • Hua-jun Cai Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Zhi-cheng Zhuang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China https://orcid.org/0000-0003-4376-5049
  • Yong Wu Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Yi-yi Zhang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Xing Liu Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Jin-fu Zhuang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Yuan-feng Yang Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Yuan Gao Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Bin Chen Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • Guo-xian Guan Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
Keywords: TCGA, colon adenocarcinoma, ferroptosis, long non-coding RNA, prognosis signature

Abstract

Ferroptosis is a form of iron-dependent programmed cell death. Regulation of ferroptosis in tumor cells is a novel treatment modality. The present study aimed to investigate ferroptosis-related long non-coding RNAs (lncRNAs) and construct a prognostic model for colon adenocarcinoma (COAD). RNA- sequencing data and ferroptosis-related genes were obtained from The Cancer Genome Atlas database and FerrDb database. COAD patients were randomly assigned to training- and validation groups. The Least Absolute Shrinkage and Selection Operator regression and Cox regression model were used to determine and develop a predictive model. The model was corroborated using the validation group and the entire group. In total, 259 ferroptosis-related genes and 905 ferroptosis-related LncRNAs were obtained. Cox model revealed and constructed seven ferroptosis-related LncRNAs signature (LINC01503, AC004687.1, AC010973.2, AP001189.3, ARRDC1-AS1, OIP5-AS1, and NCK1-DT). Patients were assigned into two groups according to the median risk score. Kaplan–Meier survival curves showed that overall survival between high- and low-risk groups was statistically significant (P<0.01). Cox multivariate analysis seven ferroptosis-related LncRNAs signature was an independent risk factor for COAD outcomes (P<0.05). The relationship between seven ferroptosis-related LncRNAs and clinicopathological features was also examined. The principal component analysis showed a difference between high- and low-risk groups intuitively. With the aid of gene set enrichment analysis, the underlying mechanisms of seven ferroptosis-related LncRNAs were uncovered, including the MAPK signaling pathway, mTOR signaling pathway, and glutathione metabolism pathway. Finally, we established and validated seven ferroptosis-related lncRNAs signature for COAD patients to predict survival. These results may provide meaningful targets for future study.

Downloads

Download data is not yet available.

Author Biographies

Hua-jun Cai, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Zhi-cheng Zhuang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Yong Wu, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Yi-yi Zhang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Xing Liu, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Jin-fu Zhuang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Yuan-feng Yang, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Yuan Gao, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Bin Chen, Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

Department of Colorectal Surgery

Development and validation of a ferroptosis-related lncRNAs prognosis signature in colon cancer
Published
2021-03-09
How to Cite
1.
Cai H- jun, Zhuang Z- cheng, Wu Y, Zhang Y- yi, Liu X, Zhuang J- fu, Yang Y- feng, Gao Y, Chen B, Guan G- xian. Development and validation of a ferroptosis-related lncRNAs prognosis signature in colon cancer. Bosn J of Basic Med Sci [Internet]. 2021Mar.9 [cited 2021Apr.19];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/5617
Section
Molecular Biology

Funding data