Circular RNA circ_0020014 contributes to osteoarthritis progression via miR-613/ADAMTS5 axis

Authors

  • Zirui Yu Department of Orthopaedics, Xi’an Hong-Hui Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi, China https://orcid.org/0000-0002-5084-9956
  • Fei Cong Department of Orthopaedics, Xi’an Hong-Hui Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi, China
  • Wentao Zhang Department of Orthopaedics, Xi’an Hong-Hui Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi, China
  • Tao Song Department of Orthopaedics, Xi’an Hong-Hui Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi, China
  • Shihui Zhang Department of Orthopaedics, Xi’an Hong-Hui Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi, China
  • Renqi Jiang Department of Orthopaedics, Xi’an Hong-Hui Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi, China

DOI:

https://doi.org/10.17305/bjbms.2021.6668

Keywords:

ADAMTS5, miR-613, circ_0020014, osteoarthritis

Abstract

Circular RNAs (circRNAs) have been shown to be significant regulators in osteoarthritis (OA), whereas the functional effect of circ_0020014 in OA remains unclear. Our goal was to try and understand the underlying regulatory mechanism of circ_0020014 in OA. The cartilage tissue was obtained from OA patients and trauma patients. Interleukin-1β (IL-1β)-treated chondrocytes (CHON-001) were used as the in vitro cellular model for OA. The expression levels of circ_0020014, microRNA-613 (miR-613), and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) were examined by real-time quantitative polymerase chain reaction (RT-qPCR). The protein level was detected using the western blot assay. Cell viability and apoptosis were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazol-3-ium bromide (MTT) and flow cytometry assays, respectively. The secretion of inflammatory cytokine was determined by enzyme-linked immunosorbent assay (ELISA). Circ_0020014 was upregulated in OA cartilage tissues and IL-1β-treated CHON-001 cells, compared with that in healthy cartilage tissues and untreated cells. IL-1β treatment induced cell injury by promoting inflammation and apoptosis, and inhibiting cell viability and extracellular matrix (ECM) accumulation in chondrocytes. Circ_0020014 knockdown significantly protected CHON-001 cells from IL-1β-induced cell dysfunction. MiR-613 was targeted by circ_0020014 and negatively regulated ADAMTS5 expression. In addition, miR-613 downregulation or ADAMTS5 overexpression partly lessened the protective effect of circ_0020014 knockdown on IL-1β-treated CHON-001 cells. Collectively, circ_0020014 acted as a miR-613 sponge to regulate ADAMTS5 expression, thereby protecting chondrocytes from IL-1β-induced inflammatory damage, which might be a novel diagnostic marker for OA.

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Circular RNA circ_0020014 contributes to osteoarthritis progression via miR-613/ADAMTS5 axis

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Published

2022-09-16

How to Cite

1.
Yu Z, Cong F, Zhang W, Song T, Zhang S, Jiang R. Circular RNA circ_0020014 contributes to osteoarthritis progression via miR-613/ADAMTS5 axis. Bosn J of Basic Med Sci [Internet]. 2022Sep.16 [cited 2022Sep.25];22(5):716-27. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/6668

Issue

Section

Molecular Biology