Exploring autophagy-related prognostic genes of Alzheimer's disease based on pathway crosstalk analysis

Authors

  • Fang Qian College of Information Engineering, Shanghai Maritime University, Shanghai, China https://orcid.org/0000-0002-3272-3890
  • Wei Kong College of Information Engineering, Shanghai Maritime University, Shanghai, China
  • Shuaiqun Wang College of Information Engineering, Shanghai Maritime University, Shanghai, China https://orcid.org/0000-0001-6274-4097

DOI:

https://doi.org/10.17305/bjbms.2021.7019

Keywords:

Alzheimer's disease (AD), Autophagy, Prognostic signature, Pathway crosstalk, Weighted gene co-expression network analysis (WGCNA), Pathway analysis method based on global influence (PAGI)

Abstract

Recent studies have shown that different signaling pathways are involved in the pathogenesis of Alzheimer's disease (AD), with complex molecular connections existing between these pathways. Autophagy is crucial for the degradation and production of pathogenic proteins in AD, and it shows link with other AD-related pathways. However, current methods for identifying potential therapeutic targets for AD are primarily based on single-gene analysis or a single signal pathway, both of which are somewhat limited. Finding other methods is necessary for providing novel underlying AD therapeutic targets. Therefore, given the central role of autophagy in AD and its interplay with its pathways, we aimed to identify prognostic genes related to autophagy within and between these pathways based on pathway crosstalk analysis. The method of pathway analysis based on global influence (PAGI) was applied to find the feature mRNAs involved in the crosstalk between autophagy and other AD-related pathways. Subsequently, the weighted gene co-expression network analysis (WGCNA) was used to construct a co-expression module of feature mRNAs and differential lncRNAs. Finally, based on 2 autophagy-related crosstalk genes (CD40 and SMAD7), we constructed a prognosis model by multivariate Cox regression, which could predict the overall survival of AD patients with medium-to-high accuracy. In conclusion, we provided an effective method for extracting autophagy-related significant genes based on pathway crosstalk in AD. We found the biomarkers valuable to the AD prognosis, which may also play an essential role in the development and treatment of AD.

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Exploring autophagy related prognostic genes of Alzheimer's disease based on pathway crosstalk analysis

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Published

2022-09-16

How to Cite

1.
Qian F, Kong W, Wang S. Exploring autophagy-related prognostic genes of Alzheimer’s disease based on pathway crosstalk analysis. Bosn J of Basic Med Sci [Internet]. 2022Sep.16 [cited 2022Sep.25];22(5):751-7. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/7019

Issue

Section

Molecular Biology