Methylation pattern of Caveolin-1 in prostate cancer as potential cfDNA biomarker

Authors

  • Lucija Škara Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Group for Research on Epigenetic Biomarkers, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Centre of Excellence for Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, Zagreb, Croatia https://orcid.org/0000-0002-1270-2355
  • Tonći Vodopić Ljudevit Jurak Clinical Department of Pathology and Cytology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia https://orcid.org/0000-0001-8865-9130
  • Ivan Pezelj Department of Urology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
  • Irena Abramovic Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Group for Research on Epigenetic Biomarkers, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Centre of Excellence for Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, Zagreb, Croatia https://orcid.org/0000-0002-0339-5007
  • Borna Vrhovec Department of Urology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia https://orcid.org/0000-0003-4126-8807
  • Alen Vrtarić Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
  • Nino Sincic Department of Biology, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Group for Research on Epigenetic Biomarkers, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Centre of Excellence for Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, Zagreb, Croatia https://orcid.org/0000-0003-2584-6654
  • Davor Tomas Ljudevit Jurak Clinical Department of Pathology and Cytology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia; Department of Pathology, University of Zagreb School of Medicine, Zagreb, Croatia
  • Stela Bulimbašić Department of Pathology, University of Zagreb School of Medicine, Zagreb, Croatia; Pathology and Cytology Department, University Hospital Center Zagreb, Zagreb, Croatia
  • Tomislav Kuliš Scientific Group for Research on Epigenetic Biomarkers, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Centre of Excellence for Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, Zagreb, Croatia; Department of Urology, University Hospital Center Zagreb, Zagreb, Croatia https://orcid.org/0000-0002-0895-5691
  • Monika Ulamec Scientific Group for Research on Epigenetic Biomarkers, University of Zagreb School of Medicine, Zagreb, Croatia; Scientific Centre of Excellence for Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, Zagreb, Croatia; Ljudevit Jurak Clinical Department of Pathology and Cytology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia; Department of Pathology, University of Zagreb School of Medicine, Zagreb, Croatia https://orcid.org/0000-0002-4843-8154

DOI:

https://doi.org/10.17305/bjbms.2022.7497

Keywords:

prostate cancer, biomarker, cfDNA methylation, CAV1

Abstract

High prevalence and mortality of prostate cancer (PCa) are well known global health issues. Novel biomarkers for better identifying patients with PCa are the subject of extensive research. Prostate specific antigen (PSA) shows low specificity in screening and diagnostics, leading to unnecessary biopsies and health costs. Eighty patients with PCa and benign prostate hyperplasia (BPH) were included in the study. We analyzed CAV1 gene expression and methylation in tissue. CAV1 cfDNA methylation from blood and seminal plasma was accessed as a potential PCa biomarker. Although methylation in blood plasma did not differ between PCa and BPH patients, methylation in seminal plasma showed better PCa biomarker performances than tPSA (AUC 0.63 vs. AUC 0.52). Discrimination of BPH and Gleason grade group 1 PCa patients from patients with higher Gleason grade groups revealed very good performance as well (AUC 0.72). CAV1 methylation is useful biomarker with potential for further seminal plasma cfDNA research, but its diagnostic accuracy should be improved, as well as general knowledge about cfDNA in seminal plasma.

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Methylation pattern of Caveolin-1 in prostate cancer as potential cfDNA biomarker

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Published

2022-08-26

How to Cite

1.
Škara L, Vodopić T, Pezelj I, Abramovic I, Vrhovec B, Vrtarić A, Sincic N, Tomas D, Bulimbašić S, Kuliš T, Ulamec M. Methylation pattern of Caveolin-1 in prostate cancer as potential cfDNA biomarker. Bosn J of Basic Med Sci [Internet]. 2022Aug.26 [cited 2022Sep.29];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/7497

Issue

Section

Translational and Clinical Research