Cytotoxic T-lymphocyte-associated protein 4 +49A/G polymorphism in Down syndrome children with Hashimoto’s thyroiditis

Authors

  • Muhammad Faizi Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
  • Nur Rochmah Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia https://orcid.org/0000-0002-9626-9615
  • Yuni Hisbiyah Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
  • Rayi Kurnia Perwitasari Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
  • Qurrota Ayuni Novia Putri Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia https://orcid.org/0000-0002-5865-6382
  • Sukmawati Basuki Institute of Tropical Disease, Universitas Airlangga, Surabaya, East Java, Indonesia https://orcid.org/0000-0002-5882-4251
  • Anang Endaryanto Faculty of Medicine, Department of Child Health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
  • Soetjipto Soetjipto Department of Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia https://orcid.org/0000-0003-2203-5565

DOI:

https://doi.org/10.17305/bb.2022.7869

Keywords:

Down syndrome, Hashimoto’s thyroiditis, CTLA-4 49A/G

Abstract

Thyroid dysfunction is the most common endocrine disorder in Down syndrome (DS) children. Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) is one of the immune regulatory genes that correlates with Hashimoto’s thyroiditis (HT). However, studies on CTLA-4 +49A/G in DS children with HT are still limited. We aimed to evaluate CTLA-4 +49A/G gene polymorphism in DS children with HT. This case-control study, conducted from February 2020 to February 2022 at Dr. Soetomo General Hospital, Surabaya, enrolled 40 DS children with HT and 50 healthy children. The DNA sequencing was performed to identify the polymorphism (Sanger sequencing). Thyroid peroxidase antibodies (TPOAb), thyroglobulin antibodies (TgAb), thyroid-stimulating hormone (TSH), and free thyroxine (FT4) levels were analyzed by enzyme-linked immunosorbent assay (ELISA). The mean age of DS children with HT was 1.78 years. Males predominated in the study population. Subjects with GG genotype were diagnosed earliest with hypothyroidism (8 months) compared with other studies. The most common thyroid dysfunction was central hypothyroidism, with TgAb positivity present in all patients. The AA genotype (odds ratio [OR] 0.265, 95% confidence interval [CI] 0.094–0.746; P = 0.012) and A allele (OR 0.472, 95% CI 0.309–0.721; P = 0.0002) were significantly more frequent in the control group. The AG genotype (OR 2.65, 95% CI 0.094–0.746; P = 0.003) and G allele (OR 2.116, 95% CI 1.386–3.23; P = 0.003) were more frequent in the DS with HT group. The age of the subjects in this study was younger than in previous studies. The AG genotype and the G allele were more prevalent in the DS with HT group and may be a risk factor in HT development in DS children. Furthermore, the AA genotype may act as a protective factor against HT in DS children.

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Cytotoxic T-lymphocyte-associated protein 4 +49A/G polymorphism in Down syndrome children with Hashimoto’s thyroiditis

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Published

2023-01-30

How to Cite

1.
Faizi M, Rochmah N, Hisbiyah Y, Perwitasari RK, Putri QAN, Basuki S, Endaryanto A, Soetjipto S. Cytotoxic T-lymphocyte-associated protein 4 +49A/G polymorphism in Down syndrome children with Hashimoto’s thyroiditis. Biomol Biomed [Internet]. 2023Jan.30 [cited 2023Mar.21];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/7869

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Section

Molecular Biology