Cytoplasmic amino acid profiles of clinical and ATCC 29213 strains of Staphylococcus aureus harvested at different growth phases

Authors

  • Mousa Alreshidi Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia
  • Hugh Dunstan InnovAAte Pty Ltd, Newcastle, NSW, Australia https://orcid.org/0000-0002-1058-8739
  • Tim Roberts Metabolic Research Group, Faculty of Science, School of Environmental and Life Sciences, University Drive, Callaghan, NSW, Australia image/svg+xml
  • Fayez Alreshidi Department of Family and Community Medicine, College of Medicine, University of Ha’il, Ha’il, Saudi Arabia image/svg+xml https://orcid.org/0000-0002-2391-9090
  • Ashfaque Hossain Department of Medical Microbiology and Immunology, RAK Medical and Health Sciences University, Ras Al Khaimah, United Arab Emirates
  • Fevzi Bardakci Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia image/svg+xml https://orcid.org/0000-0003-3377-2763
  • Mejdi Snoussi Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia; Laboratory of Genetics, Biodiversity and Valorization of Bio-Resources (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir, Tunisia https://orcid.org/0000-0002-2309-2601
  • Riadh Badraoui Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia image/svg+xml https://orcid.org/0000-0001-9054-7744
  • Mohd Adnan Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia image/svg+xml https://orcid.org/0000-0002-7080-6822
  • Sultan Alouffi Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail, Saudi Arabia
  • Mohd Saeed Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia image/svg+xml

DOI:

https://doi.org/10.17305/bb.2023.9246

Keywords:

Metabolic homeostasis, growth phases, bacterial adaptation, bacterial strains

Abstract

The objective of the present investigation was to compare potential differences in cytoplasmic amino acid levels between clinical and ATCC 29213 strains of Staphylococcus aureus (S. aureus). The two strains were grown under ideal conditions to mid-exponential and stationary growth phases, after which they were harvested to analyze their amino acid profiles. Initially, the amino acid patterns of both strains were compared at the mid-exponential phase when grown in controlled conditions. At the mid-exponential phase, both strains shared common features in cytoplasmic amino acid levels, with glutamic acid, aspartic acid, proline and alanine identified as key amino acids. However, the concentration profiles of seven amino acids exhibited major variances between the strains, even though the total cytoplasmic levels of amino acids did not alter significantly. At the stationary phase, the magnitudes of the amino acids abundant in the mid-exponential phase were altered. Aspartic acid became the most abundant amino acid in both strains accounting for 44% and 59% of the total amino acids in the clinical and ATCC 29213 strains, respectively. Lysine was the second most abundant amino acid in both strains, accounting for 16% of the total cytoplasmic amino acids, followed by glutamic acid, the concentration of which was significantly higher in the clinical strain than in the ATCC 29213 strain. Interestingly, histidine was clearly present in the clinical strain but was virtually lacking in the ATCC 29213 strain. This study reveals the dynamic diversity of amino acid levels among strains, which is an essential step toward illustrating the variability in S. aureus cytoplasmic amino acid profiles and could be significant in explaining variances among strains of S. aureus.

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Author Biography

Hugh Dunstan, InnovAAte Pty Ltd, Newcastle, NSW, Australia

Experienced Professor with a demonstrated history of working in the higher education industry. Skilled in Research Design, Biotechnology, Life Sciences, Research Management, and Scientific Writing. Strong education professional with a D.Phil focused in Biochemistry and metabolsim from University of Oxford.

Cytoplasmic amino acid profiles of clinical and ATCC 29213 strains of Staphylococcus aureus harvested at different growth phases

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Published

2023-06-04

How to Cite

1.
Alreshidi M, Dunstan H, Roberts T, Alreshidi F, Hossain A, Bardakci F, Snoussi M, Badraoui R, Adnan M, Alouffi S, Saeed M. Cytoplasmic amino acid profiles of clinical and ATCC 29213 strains of Staphylococcus aureus harvested at different growth phases. Biomol Biomed [Internet]. 2023Jun.4 [cited 2023Sep.27];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/9246

Issue

Section

Microbiology