Plasma miR-19b, miR-34a, and miR-146a expression in patients with type 2 diabetes mellitus and cataract: A pilot study

Authors

  • Adina Iuliana Milcu Doctoral School, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Discipline of Ophthalmology, Department of Surgery I, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Department of Ophthalmology, Municipal Emergency Clinical Hospital, Timisoara, Romania
  • Flavia Medana Anghel Doctoral School, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Discipline of Biochemistry, Department of Biochemistry and Pharmacology, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania https://orcid.org/0000-0002-1288-2595
  • Mirabela Romanescu Doctoral School, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Discipline of Biochemistry, Department of Biochemistry and Pharmacology, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Center for Complex Network Science, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania
  • Aimee Rodica Chis Discipline of Biochemistry, Department of Biochemistry and Pharmacology, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Center for Complex Network Science, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania
  • Andrei Anghel Discipline of Biochemistry, Department of Biochemistry and Pharmacology, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Center for Complex Network Science, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania
  • Ovidiu Boruga Discipline of Ophthalmology, Department of Surgery I, “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania; Department of Ophthalmology, Municipal Emergency Clinical Hospital, Timisoara, Romania

DOI:

https://doi.org/10.17305/bb.2023.9933

Keywords:

Cataract, diabetes mellitus (DM), diabetic retinopathy (DR), microRNAs (miRNAs), biomarker, qRT-PCR

Abstract

Cataract is among the most common ocular complications in diabetes mellitus (DM). While microRNA (miRNA) dysregulations in DM have been previously reported, consensus is still lacking concerning miRNA expression in cataract. Furthermore, the miRNA profile in diabetic cataract patients remains largely unexplored, and data on plasma expression levels are limited. Our study aimed to assess the plasma levels of three distinct miRNA species (hsa-miR-19b, hsa-miR-34a, and hsa-miR-146a) implicated in the development of cataract and/or DM.We investigated the circulating miRNA expression in DM patients diagnosed with cataract, compared to a non-DM cataract group. We employed qRT-PCR for relative quantification experiments and subsequently conducted a correlation analysis between miRNA expression levels and clinical characteristics. Our findings reveal that hsa-miR-34a and hsa-miR-146a are differentially expressed in the two cohorts. However, no significant correlation was observed between the clinical variables and miRNA levels. In summary, our results suggest a potential role for hsa-miR-34a and hsa-miR-146a in the biology of diabetic cataract.

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Plasma miR-19b, miR-34a, and miR-146a expression in patients with type 2 diabetes mellitus and cataract: A pilot study

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Published

02-05-2024

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Section

Molecular Biology

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How to Cite

1.
Plasma miR-19b, miR-34a, and miR-146a expression in patients with type 2 diabetes mellitus and cataract: A pilot study. Biomol Biomed [Internet]. 2024 May 2 [cited 2024 May 19];24(3):537–544. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/9933