Heart-type fatty acid-binding protein and its relation with morphological changes in rat myocardial damage model induced by isoproterenol

  • Sabaheta Hasić Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
  • Radivoj Jadrić Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
  • Esad Ćosović Institute for Histology and Embryology, Faculty of Medicine, University of Sarajevo
  • Emina Kiseljaković Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
  • Zakira Mornjaković Institute for Histology and Embryology, Faculty of Medicine, University of Sarajevo
  • Mira Winterhalter-Jadrić Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
Keywords: H-FABP, isoproterenol, histology, myocardial lesion score, rat

Abstract

We have investigated heart type fatty acid binding protein (H-FABP) rat serum values at different time point following subcutaneous (s.c) isoproterenol (ISO) administration and their correlation with severity of myocardial lesion. Thirty adult, male, Wistar rats were used for this study. Six rats per group were treated with a single dose of either ISO (ISO groups, dose 100 mg/kg, s.c.) at different time point (30’, 60’, 120’, 240’) or with saline (control group). Serum H-FABP was determined by enzyme-linked immunosorbent assay (ELISA) and histological analysis was performed by hematoxylin-eosin (HE) method of staining. The first serum H-FABP increase was obtained 30’ following ISO administration, but maximal value was reached after 240’. Myocardial histological changes were time-dependent and correlated with serum H-FABP values (p<0.001). The results of the study suggest that H-FABP is sensitive marker for acute rat myocardial injury and its possible inclusion in myocardial injury screening studies in rats.

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Published
2011-11-20
How to Cite
1.
Hasić S, Jadrić R, Ćosović E, Kiseljaković E, Mornjaković Z, Winterhalter-Jadrić M. Heart-type fatty acid-binding protein and its relation with morphological changes in rat myocardial damage model induced by isoproterenol. Bosn J of Basic Med Sci [Internet]. 2011Nov.20 [cited 2019Sep.18];11(4):240-4. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/2557
Section
Biochemistry