Heart-type fatty acid-binding protein and its relation with morphological changes in rat myocardial damage model induced by isoproterenol

Sabaheta Hasić; Radivoj Jadrić; Esad Ćosović; Emina Kiseljaković; Zakira Mornjaković; Mira Winterhalter-Jadrić

doi:10.17305/bjbms.2011.2557

Authors

Sabaheta Hasić Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
Radivoj Jadrić Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
Esad Ćosović Institute for Histology and Embryology, Faculty of Medicine, University of Sarajevo
Emina Kiseljaković Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo
Zakira Mornjaković Institute for Histology and Embryology, Faculty of Medicine, University of Sarajevo
Mira Winterhalter-Jadrić Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo

DOI:

https://doi.org/10.17305/bjbms.2011.2557

Keywords:

H-FABP, isoproterenol, histology, myocardial lesion score, rat

Abstract

We have investigated heart type fatty acid binding protein (H-FABP) rat serum values at different time point following subcutaneous (s.c) isoproterenol (ISO) administration and their correlation with severity of myocardial lesion. Thirty adult, male, Wistar rats were used for this study. Six rats per group were treated with a single dose of either ISO (ISO groups, dose 100 mg/kg, s.c.) at different time point (30’, 60’, 120’, 240’) or with saline (control group). Serum H-FABP was determined by enzyme-linked immunosorbent assay (ELISA) and histological analysis was performed by hematoxylin-eosin (HE) method of staining. The first serum H-FABP increase was obtained 30’ following ISO administration, but maximal value was reached after 240’. Myocardial histological changes were time-dependent and correlated with serum H-FABP values (p<0.001). The results of the study suggest that H-FABP is sensitive marker for acute rat myocardial injury and its possible inclusion in myocardial injury screening studies in rats.