Effect of topically applied sildenafil citrate on wound healing: experimental study

Koray Gürsoy; Melike Oruç; Yüksel Kankaya; Mustafa Gürhan Ulusoy; Uğur Koçer; Duygu Kankaya; Reyhan Neslihan Gürsoy; Özge Çevik; Elmas Öğüş; Vildan Fidanci

doi:10.17305/bjbms.2014.3.48

Authors

Koray Gürsoy ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC ANKARA
Melike Oruç ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
Yüksel Kankaya ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
Mustafa Gürhan Ulusoy ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
Uğur Koçer ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
Duygu Kankaya Ankara University, Pathology Department, ANKARA
Reyhan Neslihan Gürsoy Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, ANKARA
Özge Çevik Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, ANKARA, TURKEY
Elmas Öğüş Ankara Research and Training Hospital, Biochemistry Clinic, ANKARA, TURKEY
Vildan Fidanci Ankara Research and Training Hospital, Biochemistry Clinic, ANKARA, TURKEY

DOI:

https://doi.org/10.17305/bjbms.2014.3.48

Keywords:

Wound healing, Sildenafil Citrate, cGMP

Abstract

Wound healing is a complex process that necessitates organization of different cell types and several signalling molecules. The aim of this study is to evaluate the effect of different concentrations of sildenafil citrate, which decreases cGMP degradation, on wound healing by secondary intention.

This study was performed using 25 Sprague Dawley rats weighing 200-250 grams. 4 dorsal defects were created. Four different treatment modalities which were 1% and 5% sildenafil citrate gel prepared with carbopol, pure carbopol gel without any drug in it and 0,9% NaCl solution; were applied to each lesion of the same rat. Randomly selected five rats (25 rats in total) were sacrificed on 3^rd, 5^th, 7^th, 10^th, and 14^th days; and the effect of each modality was evaluated by means of defect area measurement, histopathological examination and measurement of tissue hydroxyproline levels.

Sildenafil citrate gel application decreased the defect areas in a dose independent manner starting from 3^rd day and dose dependent manner after 7^th day. By means of vascularization, sildenafil citrate increased vascularity starting from 3^rd day. The strength of acute inflammation was superior in sildenafil groups starting from 5^th day; and the amount and maturation of granulation in the wound bed, as well as the strength of chronic inflammation were superior in defects treated with sildenafil citrate as early as 7^th day.

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